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Molecular characterization of the 11th RNA segment from human group C rotavirus

Molecular characterization of the 11th RNA segment from human group C rotavirus
Molecular characterization of the 11th RNA segment from human group C rotavirus
The complete nucleotide sequence of genome segment 11 from the noncultivatable, human group C rotavirus (Bristol strain) was determined. Comparison of the nucleotide sequence of the segment termini with the consensus 5' and 3' terminal noncoding sequences of the human group C rotavirus genome revealed characteristic 5' and 3' sequences. Human group C rotavirus genome segment 11 is 613 bp long and encodes a single open reading frame of 450 nucleotides (150 amino acids) starting at nucleotide 39 and terminating at nucleotide 489, leaving a long 3' untranslated region of 124 nucleotides. The predicted translation product has a calculated molecular weight of 17.7 kD and contains four potential N-linked glycosylation sites. No significant homologies to other viral proteins were found in database searches. Hydropathy analysis predicted the human group C rotavirus genome segment 11 translation product has a hydrophilic carboxy terminus (amino acids 54-150) and a hydrophobic amino terminus (amino acids 1-53) that can be further subdivided into three short hydrophobic sequences--H1, H2, and H3. These features are analogous to the integral membrane glycoprotein NSP4 encoded by group A rotavirus gene 10.
rotavirus, dsRNA, NSP4 gene
0920-8569
239-243
Deng, Yu
d0f1af1d-6428-4b11-8fcb-ffef9f8c40f8
Fielding, Patrick A.
384d7c63-eb45-4e3a-b35b-0d5bf616c3a6
Lambden, Paul R.
4fcd536e-2d9a-4366-97c6-386e6b005698
Caul, E. Owen
177391c9-26e2-4c94-a459-d9353c2daa61
Clarke, Ian N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Deng, Yu
d0f1af1d-6428-4b11-8fcb-ffef9f8c40f8
Fielding, Patrick A.
384d7c63-eb45-4e3a-b35b-0d5bf616c3a6
Lambden, Paul R.
4fcd536e-2d9a-4366-97c6-386e6b005698
Caul, E. Owen
177391c9-26e2-4c94-a459-d9353c2daa61
Clarke, Ian N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b

Deng, Yu, Fielding, Patrick A., Lambden, Paul R., Caul, E. Owen and Clarke, Ian N. (1995) Molecular characterization of the 11th RNA segment from human group C rotavirus. Virus Genes, 10 (3), 239-243. (doi:10.1007/BF01701813). (PMID:8560785)

Record type: Article

Abstract

The complete nucleotide sequence of genome segment 11 from the noncultivatable, human group C rotavirus (Bristol strain) was determined. Comparison of the nucleotide sequence of the segment termini with the consensus 5' and 3' terminal noncoding sequences of the human group C rotavirus genome revealed characteristic 5' and 3' sequences. Human group C rotavirus genome segment 11 is 613 bp long and encodes a single open reading frame of 450 nucleotides (150 amino acids) starting at nucleotide 39 and terminating at nucleotide 489, leaving a long 3' untranslated region of 124 nucleotides. The predicted translation product has a calculated molecular weight of 17.7 kD and contains four potential N-linked glycosylation sites. No significant homologies to other viral proteins were found in database searches. Hydropathy analysis predicted the human group C rotavirus genome segment 11 translation product has a hydrophilic carboxy terminus (amino acids 54-150) and a hydrophobic amino terminus (amino acids 1-53) that can be further subdivided into three short hydrophobic sequences--H1, H2, and H3. These features are analogous to the integral membrane glycoprotein NSP4 encoded by group A rotavirus gene 10.

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More information

Published date: 1995
Keywords: rotavirus, dsRNA, NSP4 gene
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 352656
URI: http://eprints.soton.ac.uk/id/eprint/352656
ISSN: 0920-8569
PURE UUID: e56f71f7-008a-4e86-a066-da1ba44d345e
ORCID for Ian N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

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Date deposited: 05 Jun 2013 11:08
Last modified: 07 Oct 2020 02:06

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Contributors

Author: Yu Deng
Author: Patrick A. Fielding
Author: Paul R. Lambden
Author: E. Owen Caul
Author: Ian N. Clarke ORCID iD

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