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A conserved sequence motif at the 5' terminus of the Southampton virus genome is characteristic of the Caliciviridae

A conserved sequence motif at the 5' terminus of the Southampton virus genome is characteristic of the Caliciviridae
A conserved sequence motif at the 5' terminus of the Southampton virus genome is characteristic of the Caliciviridae
We have determined the 5'terminal cDNA sequence for the genome of Southampton virus, a recently characterized, human, small round-structured virus (SRSV). Genomic RNA was extracted directly from a stool sample and amplified by RT-PCR by homopolymer tailing of the 3' terminus of the cDNA. The additional sequence increases the overall length of the Southampton virus genome by 12 nucleotides, resulting in a significant change to the genome organization by extending the first large open reading frame (ORF) by 51 amino acids. The 5'terminal bases pGpT and the presence of conserved genome and putative subgenomic RNA terminal motifs are now prominent features shared between the human SRSV Southampton virus and the animal caliciviruses rabbit hemorrhagic disease virus and feline calicivirus.
0920-8569
149-152
Lambden, P.R.
e99ecc21-50d7-4a43-9e79-efba46592c77
Liu, B.L.
1e6ef423-fbfb-4c45-aee6-29b667878629
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Lambden, P.R.
e99ecc21-50d7-4a43-9e79-efba46592c77
Liu, B.L.
1e6ef423-fbfb-4c45-aee6-29b667878629
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b

Lambden, P.R., Liu, B.L. and Clarke, I.N. (1995) A conserved sequence motif at the 5' terminus of the Southampton virus genome is characteristic of the Caliciviridae. Virus Genes, 10 (2), 149-152. (doi:10.1007/BF01702595). (PMID:8560774)

Record type: Article

Abstract

We have determined the 5'terminal cDNA sequence for the genome of Southampton virus, a recently characterized, human, small round-structured virus (SRSV). Genomic RNA was extracted directly from a stool sample and amplified by RT-PCR by homopolymer tailing of the 3' terminus of the cDNA. The additional sequence increases the overall length of the Southampton virus genome by 12 nucleotides, resulting in a significant change to the genome organization by extending the first large open reading frame (ORF) by 51 amino acids. The 5'terminal bases pGpT and the presence of conserved genome and putative subgenomic RNA terminal motifs are now prominent features shared between the human SRSV Southampton virus and the animal caliciviruses rabbit hemorrhagic disease virus and feline calicivirus.

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Published date: 1995
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 352657
URI: http://eprints.soton.ac.uk/id/eprint/352657
ISSN: 0920-8569
PURE UUID: 478789ac-8d30-4d3f-a0cc-4b79f6221413
ORCID for I.N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

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Date deposited: 04 Jun 2013 12:47
Last modified: 06 Jun 2018 13:19

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Contributors

Author: P.R. Lambden
Author: B.L. Liu
Author: I.N. Clarke ORCID iD

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