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Microarrays: truths and consequences

Microarrays: truths and consequences
Microarrays: truths and consequences
For many, analysis of a microarray experiment starts with a spreadsheet of expression levels. While great attention is duly paid to RNA extraction, preparation and hybridization, relatively little care is devoted to extraction of expression levels from the fluorescent image. By delegating this step to a click of the mouse the exact extraction process is masked and researchers may be unwittingly compromising their data. In this review, we describe the most common mistakes committed on the path from the image to the spreadsheet and their impact on data quality. Remedies are further proposed for most of the popular microarray platforms in use today.
1-9
Šášik, R.
aa1812ed-0cfc-4ce0-a993-283ab23f58a9
Woelk, C.H.
4d3af0fd-658f-4626-b3b5-49a6192bcf7d
Corbeil, J.
155026c2-1fba-4224-ac98-5b8d0dd4cf0d
Šášik, R.
aa1812ed-0cfc-4ce0-a993-283ab23f58a9
Woelk, C.H.
4d3af0fd-658f-4626-b3b5-49a6192bcf7d
Corbeil, J.
155026c2-1fba-4224-ac98-5b8d0dd4cf0d

Šášik, R., Woelk, C.H. and Corbeil, J. (2004) Microarrays: truths and consequences. Journal of Molecular Endocrinology, 33 (1), 1-9. (doi:10.1677/jme.0.0330001).

Record type: Article

Abstract

For many, analysis of a microarray experiment starts with a spreadsheet of expression levels. While great attention is duly paid to RNA extraction, preparation and hybridization, relatively little care is devoted to extraction of expression levels from the fluorescent image. By delegating this step to a click of the mouse the exact extraction process is masked and researchers may be unwittingly compromising their data. In this review, we describe the most common mistakes committed on the path from the image to the spreadsheet and their impact on data quality. Remedies are further proposed for most of the popular microarray platforms in use today.

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Published date: 1 August 2004
Organisations: Clinical & Experimental Sciences

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Local EPrints ID: 352758
URI: https://eprints.soton.ac.uk/id/eprint/352758
PURE UUID: aec0efb5-4373-4614-976d-0ce1656b3820

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Date deposited: 04 Jun 2013 10:53
Last modified: 18 Jul 2017 04:12

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Contributors

Author: R. Šášik
Author: C.H. Woelk
Author: J. Corbeil

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