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Peptide nucleic acid probes with charged photocleavable mass markers: towards PNA-based MALDI-TOF MS genetic analysis

Peptide nucleic acid probes with charged photocleavable mass markers: towards PNA-based MALDI-TOF MS genetic analysis
Peptide nucleic acid probes with charged photocleavable mass markers: towards PNA-based MALDI-TOF MS genetic analysis
Halogen-labelled peptide organic acid (HPOA) monomers have been synthesised and incorporated into sequence-specific peptide nucleic acid (PNA) probes. Three different types of probe have been prepared; the unmodified PNA probe, the PNA probe with a mass marker, and the PNA probe with photocleavable mass marker. All three types of probe have been used in model studies to develop a mass spectrometry-based hybridisation assay for detection of point mutations in DNA.
1949-095X
27-35
Ball, Rachel J.
5ca0dc05-e0ae-4d08-a947-e84fa7495bd4
Green, Philip S.
17591259-7a2c-4fdd-ba4a-fc952504f846
Gale, Nittaya
eead6253-2431-407b-ab6b-92e35d41c3ef
Langley, G. John
7ac80d61-b91d-4261-ad17-255f94ea21ea
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Ball, Rachel J.
5ca0dc05-e0ae-4d08-a947-e84fa7495bd4
Green, Philip S.
17591259-7a2c-4fdd-ba4a-fc952504f846
Gale, Nittaya
eead6253-2431-407b-ab6b-92e35d41c3ef
Langley, G. John
7ac80d61-b91d-4261-ad17-255f94ea21ea
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89

Ball, Rachel J., Green, Philip S., Gale, Nittaya, Langley, G. John and Brown, Tom (2010) Peptide nucleic acid probes with charged photocleavable mass markers: towards PNA-based MALDI-TOF MS genetic analysis. Artificial DNA: PNA & XNA, 1 (1), 27-35. (doi:10.4161/adna.1.1.12199).

Record type: Article

Abstract

Halogen-labelled peptide organic acid (HPOA) monomers have been synthesised and incorporated into sequence-specific peptide nucleic acid (PNA) probes. Three different types of probe have been prepared; the unmodified PNA probe, the PNA probe with a mass marker, and the PNA probe with photocleavable mass marker. All three types of probe have been used in model studies to develop a mass spectrometry-based hybridisation assay for detection of point mutations in DNA.

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More information

Published date: July 2010
Organisations: Organic Chemistry: Synthesis, Catalysis and Flow, Chemistry, Faculty of Natural and Environmental Sciences

Identifiers

Local EPrints ID: 352897
URI: https://eprints.soton.ac.uk/id/eprint/352897
ISSN: 1949-095X
PURE UUID: bd769d9f-9c5c-4df3-acf9-37a4ca071258
ORCID for G. John Langley: ORCID iD orcid.org/0000-0002-8323-7235

Catalogue record

Date deposited: 22 May 2013 14:05
Last modified: 06 Jun 2018 13:11

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