The University of Southampton
University of Southampton Institutional Repository

The effects of two weeks of recombinant growth hormone administration on the response of IGF-I and N-terminal pro-peptide of collagen type III (P-III-NP) during a single bout of high resistance exercise in resistance trained young men

The effects of two weeks of recombinant growth hormone administration on the response of IGF-I and N-terminal pro-peptide of collagen type III (P-III-NP) during a single bout of high resistance exercise in resistance trained young men
The effects of two weeks of recombinant growth hormone administration on the response of IGF-I and N-terminal pro-peptide of collagen type III (P-III-NP) during a single bout of high resistance exercise in resistance trained young men
OBJECTIVE: Recombinant human growth hormone (rhGH) is used by some athletes and body builders with the aim of enhancing performance, building muscle and improving physique. Detection of the misuse of rhGH has proved difficult for a number of reasons. One of these is the effect of preceding exercise. In this randomised, double blind placebo-controlled study, we determined the effects of rhGH administration in male amateur athletes on two candidate markers of rhGH abuse, IGF-I and N-terminal pro-peptide of collagen type III (P-III-NP), following a bout of weightlifting exercise.

DESIGN: Sixteen men entered a four-week general weight training programme to homogenise their activity profile. They then undertook repeated bouts of standardised leg press weightlifting exercise (AHRET-acute heavy resistance exercise test). Blood samples were taken before and up to one hour after the AHRET. After the first laboratory visit (Test 1), the subjects were randomly assigned to receive daily injections of either rhGH (0.1IUkg(-1)day(-1)) or placebo for two weeks. The AHRET was repeated after the two-week dosing period (Test 2) and a further test was undertaken following a one-week washout (Test 3).

RESULTS: There was no effect of exercise on either IGF-I or P-III-NP in any test. Both markers were markedly elevated at Test 2 (p<0.001), with P-III-NP remaining elevated at Test 3 in the GH administration group (p<0.05). Application of the GH-2000 discriminant function positively identified GH administration in 17 of 40 blood samples taken at Test 2 from the rhGH group and none from the placebo group.

CONCLUSION: The data show that rhGH results in elevated levels of IGF-I and P-III-NP in well-trained individuals and that leg press weightlifting exercise does not affect these markers. The GH-2000 discriminant function identified four of eight subjects taking rhGH with no false positive results.
growth hormone, Igf-1, P-III-NP, resistance exercise, anti-doping
1096-6374
76-80
Velloso, C.P.
40df6567-c248-436b-809d-d969541a47a7
Aperghis, M.
0e8c7ecd-6c39-4893-a824-976ab0658669
Godfrey, R.
8ab258a9-e619-4028-86b5-ed4a10acc5a2
Blazevich, A J.
e390db03-8ba0-4664-9fa1-d183e1500da5
Bartlett, C.
fdad05d5-46a9-44f1-8554-65d78f0c4523
Cowan, D.
bfceb464-1233-4510-b066-c1df947245f2
Holt, R.I.G.
d54202e1-fcf6-4a17-a320-9f32d7024393
Bouloux, P.
c2c7102d-5923-4694-a4bc-81ee27b0a612
Harridgre, S.D.R.
4a6709c8-08e8-41c7-b9e6-044bf7825ec5
Goldspink, G.
402c1108-7556-4ba9-a835-a01d6e0a1609
Velloso, C.P.
40df6567-c248-436b-809d-d969541a47a7
Aperghis, M.
0e8c7ecd-6c39-4893-a824-976ab0658669
Godfrey, R.
8ab258a9-e619-4028-86b5-ed4a10acc5a2
Blazevich, A J.
e390db03-8ba0-4664-9fa1-d183e1500da5
Bartlett, C.
fdad05d5-46a9-44f1-8554-65d78f0c4523
Cowan, D.
bfceb464-1233-4510-b066-c1df947245f2
Holt, R.I.G.
d54202e1-fcf6-4a17-a320-9f32d7024393
Bouloux, P.
c2c7102d-5923-4694-a4bc-81ee27b0a612
Harridgre, S.D.R.
4a6709c8-08e8-41c7-b9e6-044bf7825ec5
Goldspink, G.
402c1108-7556-4ba9-a835-a01d6e0a1609

Velloso, C.P., Aperghis, M., Godfrey, R., Blazevich, A J., Bartlett, C., Cowan, D., Holt, R.I.G., Bouloux, P., Harridgre, S.D.R. and Goldspink, G. (2013) The effects of two weeks of recombinant growth hormone administration on the response of IGF-I and N-terminal pro-peptide of collagen type III (P-III-NP) during a single bout of high resistance exercise in resistance trained young men. Growth Hormone and IGF Research, 23 (3), 76-80. (doi:10.1016/j.ghir.2013.01.002). (PMID:23433656)

Record type: Article

Abstract

OBJECTIVE: Recombinant human growth hormone (rhGH) is used by some athletes and body builders with the aim of enhancing performance, building muscle and improving physique. Detection of the misuse of rhGH has proved difficult for a number of reasons. One of these is the effect of preceding exercise. In this randomised, double blind placebo-controlled study, we determined the effects of rhGH administration in male amateur athletes on two candidate markers of rhGH abuse, IGF-I and N-terminal pro-peptide of collagen type III (P-III-NP), following a bout of weightlifting exercise.

DESIGN: Sixteen men entered a four-week general weight training programme to homogenise their activity profile. They then undertook repeated bouts of standardised leg press weightlifting exercise (AHRET-acute heavy resistance exercise test). Blood samples were taken before and up to one hour after the AHRET. After the first laboratory visit (Test 1), the subjects were randomly assigned to receive daily injections of either rhGH (0.1IUkg(-1)day(-1)) or placebo for two weeks. The AHRET was repeated after the two-week dosing period (Test 2) and a further test was undertaken following a one-week washout (Test 3).

RESULTS: There was no effect of exercise on either IGF-I or P-III-NP in any test. Both markers were markedly elevated at Test 2 (p<0.001), with P-III-NP remaining elevated at Test 3 in the GH administration group (p<0.05). Application of the GH-2000 discriminant function positively identified GH administration in 17 of 40 blood samples taken at Test 2 from the rhGH group and none from the placebo group.

CONCLUSION: The data show that rhGH results in elevated levels of IGF-I and P-III-NP in well-trained individuals and that leg press weightlifting exercise does not affect these markers. The GH-2000 discriminant function identified four of eight subjects taking rhGH with no false positive results.

This record has no associated files available for download.

More information

e-pub ahead of print date: 19 February 2013
Published date: 23 June 2013
Keywords: growth hormone, Igf-1, P-III-NP, resistance exercise, anti-doping
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 353167
URI: http://eprints.soton.ac.uk/id/eprint/353167
ISSN: 1096-6374
PURE UUID: 41539fb9-bed8-434d-9c30-3803c7588d27
ORCID for R.I.G. Holt: ORCID iD orcid.org/0000-0001-8911-6744

Catalogue record

Date deposited: 31 May 2013 13:37
Last modified: 09 Jan 2022 03:06

Export record

Altmetrics

Contributors

Author: C.P. Velloso
Author: M. Aperghis
Author: R. Godfrey
Author: A J. Blazevich
Author: C. Bartlett
Author: D. Cowan
Author: R.I.G. Holt ORCID iD
Author: P. Bouloux
Author: S.D.R. Harridgre
Author: G. Goldspink

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×