Production of polymeric micelles by microfluidic technology for combined drug delivery: application to osteogenic differentiation of human periodontal ligament mesenchymal stem cells (hPDLSCs)
Production of polymeric micelles by microfluidic technology for combined drug delivery: application to osteogenic differentiation of human periodontal ligament mesenchymal stem cells (hPDLSCs)
The current paper reports the production of polymeric micelles (PMs), based on pluronic block-copolymers, as drug carriers, precisely controlling the cellular delivery of drugs with various physico-chemical characteristics. PMs were produced with a microfluidic platform to exploit further control on the size characteristic of the PMs.
PMs were designed for the co-delivery of dexamethasone (Dex) and ascorbyl-palmitate (AP) to in vitro cultured human periodontal ligament mesenchymal stem cells (hPDLSCs) for the combined induction of osteogenic differentiation.
Mixtures of block-copolymers and drugs in organic, water miscible solvent, were conveniently converted in PMs within microfluidic channel leveraging the fast mixing at the microscale. Our results demonstrated that the drugs can be efficiently co-encapsulated in PMs and that different production parameters can be adjusted in order to modulate the PM characteristics. The comparative analysis of PM produced by microfluidic and conventional procedures confirmed that the use of microfluidics platforms allowed the production of PMs in a robust manner with improved controllability, reproducibility, smaller size and polydispersity.
Finally, the analysis of the effect of PMs, containing Dex and AP, on the osteogenic differentiation of hPDLSCs is reported. The data demonstrated the effectiveness and safety of PM treatment on hPDLSC.
In conclusion, this report indicates that microfluidic approach represents an innovative and useful method for PM controlled preparation, warrant further evaluation as general methodology for the production of colloidal systems for the simultaneous drug delivery.
195-206
Capretto, L.
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Mazzitelli, S.
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Colombo, G.
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Piva, R.
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Penolazzi, L.
b16bb7cb-3277-48dd-851e-7b25661a6202
Vecchiatini, R.
c123f4db-b6c8-49c1-ab87-010f4a659a8d
Zhang, X.
d7cf1181-3276-4da1-9150-e212b333abb1
Nastruzzi, C.
10305e34-5e70-461a-9703-deeab9985684
20 January 2013
Capretto, L.
eef08c1d-b2bf-43f1-a310-39d7405a1f0b
Mazzitelli, S.
7ad66607-35de-4950-a089-ad8c53971a93
Colombo, G.
8f791f8e-bb1c-432d-bf27-e2e1cfcd54b8
Piva, R.
acab6114-2ff1-4489-b2cd-b1f07379878b
Penolazzi, L.
b16bb7cb-3277-48dd-851e-7b25661a6202
Vecchiatini, R.
c123f4db-b6c8-49c1-ab87-010f4a659a8d
Zhang, X.
d7cf1181-3276-4da1-9150-e212b333abb1
Nastruzzi, C.
10305e34-5e70-461a-9703-deeab9985684
Capretto, L., Mazzitelli, S., Colombo, G., Piva, R., Penolazzi, L., Vecchiatini, R., Zhang, X. and Nastruzzi, C.
(2013)
Production of polymeric micelles by microfluidic technology for combined drug delivery: application to osteogenic differentiation of human periodontal ligament mesenchymal stem cells (hPDLSCs).
[in special issue: Innovating Pharmaceutical Technologies]
International Journal of Pharmaceutics, 440 (2), .
(doi:10.1016/j.ijpharm.2012.07.057).
(PMID:22884778)
Abstract
The current paper reports the production of polymeric micelles (PMs), based on pluronic block-copolymers, as drug carriers, precisely controlling the cellular delivery of drugs with various physico-chemical characteristics. PMs were produced with a microfluidic platform to exploit further control on the size characteristic of the PMs.
PMs were designed for the co-delivery of dexamethasone (Dex) and ascorbyl-palmitate (AP) to in vitro cultured human periodontal ligament mesenchymal stem cells (hPDLSCs) for the combined induction of osteogenic differentiation.
Mixtures of block-copolymers and drugs in organic, water miscible solvent, were conveniently converted in PMs within microfluidic channel leveraging the fast mixing at the microscale. Our results demonstrated that the drugs can be efficiently co-encapsulated in PMs and that different production parameters can be adjusted in order to modulate the PM characteristics. The comparative analysis of PM produced by microfluidic and conventional procedures confirmed that the use of microfluidics platforms allowed the production of PMs in a robust manner with improved controllability, reproducibility, smaller size and polydispersity.
Finally, the analysis of the effect of PMs, containing Dex and AP, on the osteogenic differentiation of hPDLSCs is reported. The data demonstrated the effectiveness and safety of PM treatment on hPDLSC.
In conclusion, this report indicates that microfluidic approach represents an innovative and useful method for PM controlled preparation, warrant further evaluation as general methodology for the production of colloidal systems for the simultaneous drug delivery.
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IntlJPharmceu PMs for combined drug delivery 2013v440p195-206.pdf
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Accepted/In Press date: 23 July 2012
e-pub ahead of print date: 3 August 2012
Published date: 20 January 2013
Organisations:
Bioengineering Group
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Local EPrints ID: 353230
URI: http://eprints.soton.ac.uk/id/eprint/353230
ISSN: 0378-5173
PURE UUID: 985b188b-4aa1-4d91-8ca6-cb831af3c0dc
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Date deposited: 03 Jun 2013 07:54
Last modified: 15 Mar 2024 03:29
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Contributors
Author:
L. Capretto
Author:
S. Mazzitelli
Author:
G. Colombo
Author:
R. Piva
Author:
L. Penolazzi
Author:
R. Vecchiatini
Author:
C. Nastruzzi
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