Clonal expansion within pneumococcal serotype 6C after use of seven-valent vaccine
Clonal expansion within pneumococcal serotype 6C after use of seven-valent vaccine
Streptococcus pneumoniae causes invasive infections, primarily at the extremes of life. A seven-valent conjugate vaccine (PCV7) is used to protect against invasive pneumococcal disease in children. Within three years of PCV7 introduction, we observed a fourfold increase in serotype 6C carriage, predominantly due to a single clone. We determined the whole-genome sequences of nineteen S. pneumoniae serotype 6C isolates, from both carriage (n = 15) and disease (n = 4) states, to investigate the emergence of serotype 6C in our population, focusing on a single multi-locus sequence type (MLST) clonal complex 395 (CC395). A phylogenetic network was constructed to identify different lineages, followed by analysis of variability in gene sets and sequences. Serotype 6C isolates from this single geographical site fell into four broad phylogenetically distinct lineages. Variation was seen in the 6C capsular locus and in sequences of genes encoding surface proteins. The largest clonal complex was characterised by the presence of lantibiotic synthesis locus. In our population, the 6C capsular locus has been introduced into multiple lineages by independent capsular switching events. However, rapid clonal expansion has occurred within a single MLST clonal complex. Worryingly, plasticity exists within current and potential vaccine-associated loci, a consideration for future vaccine use, target selection and design.
e64731
Loman, Nicholas J.
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Gladstone, Rebecca
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Constantinidou, Chrystala
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Tocheva, Anna S.
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Jefferies, Johanna M.C.
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Faust, S.N.
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O'Connor, Leigh
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Chan, Jacqueline
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Pallen, Mark
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Clarke, S.C.
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28 May 2013
Loman, Nicholas J.
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Gladstone, Rebecca
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Constantinidou, Chrystala
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Tocheva, Anna S.
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Jefferies, Johanna M.C.
9468e292-0b41-412d-9470-944e257c7bcf
Faust, S.N.
f97df780-9f9b-418e-b349-7adf63e150c1
O'Connor, Leigh
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Chan, Jacqueline
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Pallen, Mark
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Clarke, S.C.
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Loman, Nicholas J., Gladstone, Rebecca, Constantinidou, Chrystala, Tocheva, Anna S., Jefferies, Johanna M.C., Faust, S.N., O'Connor, Leigh, Chan, Jacqueline, Pallen, Mark and Clarke, S.C.
(2013)
Clonal expansion within pneumococcal serotype 6C after use of seven-valent vaccine.
PLoS ONE, 8 (5), .
(doi:10.1371/journal.pone.0064731).
(PMID:23724086)
Abstract
Streptococcus pneumoniae causes invasive infections, primarily at the extremes of life. A seven-valent conjugate vaccine (PCV7) is used to protect against invasive pneumococcal disease in children. Within three years of PCV7 introduction, we observed a fourfold increase in serotype 6C carriage, predominantly due to a single clone. We determined the whole-genome sequences of nineteen S. pneumoniae serotype 6C isolates, from both carriage (n = 15) and disease (n = 4) states, to investigate the emergence of serotype 6C in our population, focusing on a single multi-locus sequence type (MLST) clonal complex 395 (CC395). A phylogenetic network was constructed to identify different lineages, followed by analysis of variability in gene sets and sequences. Serotype 6C isolates from this single geographical site fell into four broad phylogenetically distinct lineages. Variation was seen in the 6C capsular locus and in sequences of genes encoding surface proteins. The largest clonal complex was characterised by the presence of lantibiotic synthesis locus. In our population, the 6C capsular locus has been introduced into multiple lineages by independent capsular switching events. However, rapid clonal expansion has occurred within a single MLST clonal complex. Worryingly, plasticity exists within current and potential vaccine-associated loci, a consideration for future vaccine use, target selection and design.
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Published date: 28 May 2013
Organisations:
Faculty of Medicine
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Local EPrints ID: 353476
URI: http://eprints.soton.ac.uk/id/eprint/353476
ISSN: 1932-6203
PURE UUID: 3dcc269c-02d7-4959-8944-b1819f73271d
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Date deposited: 10 Jun 2013 09:40
Last modified: 15 Mar 2024 03:26
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Author:
Nicholas J. Loman
Author:
Rebecca Gladstone
Author:
Chrystala Constantinidou
Author:
Anna S. Tocheva
Author:
Johanna M.C. Jefferies
Author:
Leigh O'Connor
Author:
Jacqueline Chan
Author:
Mark Pallen
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