The University of Southampton
University of Southampton Institutional Repository

Clonal expansion within pneumococcal serotype 6C after use of seven-valent vaccine

Clonal expansion within pneumococcal serotype 6C after use of seven-valent vaccine
Clonal expansion within pneumococcal serotype 6C after use of seven-valent vaccine
Streptococcus pneumoniae causes invasive infections, primarily at the extremes of life. A seven-valent conjugate vaccine (PCV7) is used to protect against invasive pneumococcal disease in children. Within three years of PCV7 introduction, we observed a fourfold increase in serotype 6C carriage, predominantly due to a single clone. We determined the whole-genome sequences of nineteen S. pneumoniae serotype 6C isolates, from both carriage (n = 15) and disease (n = 4) states, to investigate the emergence of serotype 6C in our population, focusing on a single multi-locus sequence type (MLST) clonal complex 395 (CC395). A phylogenetic network was constructed to identify different lineages, followed by analysis of variability in gene sets and sequences. Serotype 6C isolates from this single geographical site fell into four broad phylogenetically distinct lineages. Variation was seen in the 6C capsular locus and in sequences of genes encoding surface proteins. The largest clonal complex was characterised by the presence of lantibiotic synthesis locus. In our population, the 6C capsular locus has been introduced into multiple lineages by independent capsular switching events. However, rapid clonal expansion has occurred within a single MLST clonal complex. Worryingly, plasticity exists within current and potential vaccine-associated loci, a consideration for future vaccine use, target selection and design.

1932-6203
e64731
Loman, Nicholas J.
7c0b912a-a6e8-42c6-94dd-17103158c81b
Gladstone, Rebecca
6a2011bf-2561-4956-9928-46e6b927ba6d
Constantinidou, Chrystala
04334f2a-f2d1-4d69-8fa4-104446124cf2
Tocheva, Anna S.
b7da2781-6124-4e4e-9ef1-a98c5a166927
Jefferies, Johanna M.C.
9468e292-0b41-412d-9470-944e257c7bcf
Faust, S.N.
f97df780-9f9b-418e-b349-7adf63e150c1
O'Connor, Leigh
b9b3f17e-e113-42df-a3f5-068d18a0ef2b
Chan, Jacqueline
4cfefcab-d828-42dd-8952-9a2e95a09901
Pallen, Mark
2651c945-ec13-4034-9c39-7ae80b3cc3bf
Clarke, S.C.
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Loman, Nicholas J.
7c0b912a-a6e8-42c6-94dd-17103158c81b
Gladstone, Rebecca
6a2011bf-2561-4956-9928-46e6b927ba6d
Constantinidou, Chrystala
04334f2a-f2d1-4d69-8fa4-104446124cf2
Tocheva, Anna S.
b7da2781-6124-4e4e-9ef1-a98c5a166927
Jefferies, Johanna M.C.
9468e292-0b41-412d-9470-944e257c7bcf
Faust, S.N.
f97df780-9f9b-418e-b349-7adf63e150c1
O'Connor, Leigh
b9b3f17e-e113-42df-a3f5-068d18a0ef2b
Chan, Jacqueline
4cfefcab-d828-42dd-8952-9a2e95a09901
Pallen, Mark
2651c945-ec13-4034-9c39-7ae80b3cc3bf
Clarke, S.C.
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17

Loman, Nicholas J., Gladstone, Rebecca, Constantinidou, Chrystala, Tocheva, Anna S., Jefferies, Johanna M.C., Faust, S.N., O'Connor, Leigh, Chan, Jacqueline, Pallen, Mark and Clarke, S.C. (2013) Clonal expansion within pneumococcal serotype 6C after use of seven-valent vaccine. PLoS ONE, 8 (5), e64731. (doi:10.1371/journal.pone.0064731). (PMID:23724086)

Record type: Article

Abstract

Streptococcus pneumoniae causes invasive infections, primarily at the extremes of life. A seven-valent conjugate vaccine (PCV7) is used to protect against invasive pneumococcal disease in children. Within three years of PCV7 introduction, we observed a fourfold increase in serotype 6C carriage, predominantly due to a single clone. We determined the whole-genome sequences of nineteen S. pneumoniae serotype 6C isolates, from both carriage (n = 15) and disease (n = 4) states, to investigate the emergence of serotype 6C in our population, focusing on a single multi-locus sequence type (MLST) clonal complex 395 (CC395). A phylogenetic network was constructed to identify different lineages, followed by analysis of variability in gene sets and sequences. Serotype 6C isolates from this single geographical site fell into four broad phylogenetically distinct lineages. Variation was seen in the 6C capsular locus and in sequences of genes encoding surface proteins. The largest clonal complex was characterised by the presence of lantibiotic synthesis locus. In our population, the 6C capsular locus has been introduced into multiple lineages by independent capsular switching events. However, rapid clonal expansion has occurred within a single MLST clonal complex. Worryingly, plasticity exists within current and potential vaccine-associated loci, a consideration for future vaccine use, target selection and design.

Other
fetchObject.action_uri=info_doi%2F10.1371%2Fjournal.pone.0064731&representation=PDF - Version of Record
Available under License Other.
Download (358kB)

More information

Published date: 28 May 2013
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 353476
URI: http://eprints.soton.ac.uk/id/eprint/353476
ISSN: 1932-6203
PURE UUID: 3dcc269c-02d7-4959-8944-b1819f73271d
ORCID for S.N. Faust: ORCID iD orcid.org/0000-0003-3410-7642
ORCID for S.C. Clarke: ORCID iD orcid.org/0000-0002-7009-1548

Catalogue record

Date deposited: 10 Jun 2013 09:40
Last modified: 18 Feb 2021 17:08

Export record

Altmetrics

Contributors

Author: Nicholas J. Loman
Author: Rebecca Gladstone
Author: Chrystala Constantinidou
Author: Anna S. Tocheva
Author: Johanna M.C. Jefferies
Author: S.N. Faust ORCID iD
Author: Leigh O'Connor
Author: Jacqueline Chan
Author: Mark Pallen
Author: S.C. Clarke ORCID iD

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×