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Performance of tests for latent tuberculosis in different groups of immunocompromised patients

Performance of tests for latent tuberculosis in different groups of immunocompromised patients
Performance of tests for latent tuberculosis in different groups of immunocompromised patients
BACKGROUND: Immunocompromised persons infected with Mycobacterium tuberculosis (MTB) have increased risk of tuberculosis (TB) reactivation, but their management is hampered by the occurrence of false-negative results of the tuberculin skin test (TST). The T-cell interferon (IFN)-gamma release blood assays T-SPOT.TB (TS.TB) [Oxford Immunotec; Abingdon, UK] and QuantiFERON-TB Gold In-Tube (QFT-IT) [Cellestis Ltd; Carnegie, VIC, Australia] might improve diagnostic accuracy for latent TB infection (LTBI) in high-risk persons, although their performance in different groups of immunocompromised patients is largely unknown.

METHODS AND RESULTS: Over a 1-year period, we prospectively enrolled patients in three different immunosuppressed groups, as follows: 120 liver transplantation candidates (LTCs); 116 chronically HIV-infected persons; and 95 patients with hematologic malignancies (HMs). TST, TS.TB, and QFT-IT were simultaneously performed, their results were compared, and intertest agreement was evaluated. Overall, TST provided fewer positive results (10.9%) than TS.TB (18.4%; p < 0.001) and QFT-IT (15.1%; p = 0.033). Significantly fewer HIV-infected individuals had at least one positive test (9.5%) compared with LTCs (35.8%; p < 0.001) and patients with HMs (29.5%; p < 0.001). Diagnostic agreement between tests was moderate (kappa = 0.40 to 0.65) and decreased in the HIV-infected group when the results of the TS.TB were compared with either TST (kappa = 0.16) or QFT-IT (kappa = 0.19). Indeterminate blood test results due to low positive control values were significantly more frequent with QFT-IT (7.2%) than with TS.TB (0.6%; p < 0.001).

CONCLUSIONS: Blood tests identified significantly more patients as being infected with MTB than TST, although diagnostic agreement varied across groups. Based on these results, we recommend tailoring application of the new blood IFN-gamma assays for LTBI in different high-risk groups and advise caution in their current use in immunosuppressed patients.
0012-3692
198-204
Richeldi, L.
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Losi, M.
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D'Amico, R.
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Luppi, M.
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Ferrari, A.
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Mussini, C.
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Codeluppi, M.
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Cocchi, S.
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Prati, F.
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Paci, V.
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Meacci, M.
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Meccugni, B.
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Rumpianesi, F.
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Roversi, P.
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Ceri, S.
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Luppi, F.
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Ferrara, G.
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Latorre, I.
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Gerunda, G.E.
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Torelli, G.
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Esposito, R.
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Fabbri, L.M.
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Richeldi, L.
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Losi, M.
5d48476e-5760-47eb-bd74-573132186a06
D'Amico, R.
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Luppi, M.
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Ferrari, A.
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Mussini, C.
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Codeluppi, M.
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Cocchi, S.
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Prati, F.
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Paci, V.
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Meacci, M.
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Meccugni, B.
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Rumpianesi, F.
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Roversi, P.
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Ceri, S.
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Luppi, F.
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Ferrara, G.
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Latorre, I.
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Gerunda, G.E.
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Torelli, G.
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Esposito, R.
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Fabbri, L.M.
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Richeldi, L., Losi, M., D'Amico, R., Luppi, M., Ferrari, A., Mussini, C., Codeluppi, M., Cocchi, S., Prati, F., Paci, V., Meacci, M., Meccugni, B., Rumpianesi, F., Roversi, P., Ceri, S., Luppi, F., Ferrara, G., Latorre, I., Gerunda, G.E., Torelli, G., Esposito, R. and Fabbri, L.M. (2009) Performance of tests for latent tuberculosis in different groups of immunocompromised patients. Chest, 136 (1), 198-204. (doi:10.1378/chest.08-2575). (PMID:19318676)

Record type: Article

Abstract

BACKGROUND: Immunocompromised persons infected with Mycobacterium tuberculosis (MTB) have increased risk of tuberculosis (TB) reactivation, but their management is hampered by the occurrence of false-negative results of the tuberculin skin test (TST). The T-cell interferon (IFN)-gamma release blood assays T-SPOT.TB (TS.TB) [Oxford Immunotec; Abingdon, UK] and QuantiFERON-TB Gold In-Tube (QFT-IT) [Cellestis Ltd; Carnegie, VIC, Australia] might improve diagnostic accuracy for latent TB infection (LTBI) in high-risk persons, although their performance in different groups of immunocompromised patients is largely unknown.

METHODS AND RESULTS: Over a 1-year period, we prospectively enrolled patients in three different immunosuppressed groups, as follows: 120 liver transplantation candidates (LTCs); 116 chronically HIV-infected persons; and 95 patients with hematologic malignancies (HMs). TST, TS.TB, and QFT-IT were simultaneously performed, their results were compared, and intertest agreement was evaluated. Overall, TST provided fewer positive results (10.9%) than TS.TB (18.4%; p < 0.001) and QFT-IT (15.1%; p = 0.033). Significantly fewer HIV-infected individuals had at least one positive test (9.5%) compared with LTCs (35.8%; p < 0.001) and patients with HMs (29.5%; p < 0.001). Diagnostic agreement between tests was moderate (kappa = 0.40 to 0.65) and decreased in the HIV-infected group when the results of the TS.TB were compared with either TST (kappa = 0.16) or QFT-IT (kappa = 0.19). Indeterminate blood test results due to low positive control values were significantly more frequent with QFT-IT (7.2%) than with TS.TB (0.6%; p < 0.001).

CONCLUSIONS: Blood tests identified significantly more patients as being infected with MTB than TST, although diagnostic agreement varied across groups. Based on these results, we recommend tailoring application of the new blood IFN-gamma assays for LTBI in different high-risk groups and advise caution in their current use in immunosuppressed patients.

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Published date: 24 March 2009
Organisations: Faculty of Medicine

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Local EPrints ID: 353512
URI: https://eprints.soton.ac.uk/id/eprint/353512
ISSN: 0012-3692
PURE UUID: fbe757c9-8455-48af-bff1-ce0ee53ba9e9

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Date deposited: 10 Jun 2013 12:01
Last modified: 18 Jul 2017 04:03

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Contributors

Author: L. Richeldi
Author: M. Losi
Author: R. D'Amico
Author: M. Luppi
Author: A. Ferrari
Author: C. Mussini
Author: M. Codeluppi
Author: S. Cocchi
Author: F. Prati
Author: V. Paci
Author: M. Meacci
Author: B. Meccugni
Author: F. Rumpianesi
Author: P. Roversi
Author: S. Ceri
Author: F. Luppi
Author: G. Ferrara
Author: I. Latorre
Author: G.E. Gerunda
Author: G. Torelli
Author: R. Esposito
Author: L.M. Fabbri

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