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Aberrant DNA methylation profile of chronic and transformed classic philadelphia-negative myeloproliferative neoplasms

Aberrant DNA methylation profile of chronic and transformed classic philadelphia-negative myeloproliferative neoplasms
Aberrant DNA methylation profile of chronic and transformed classic philadelphia-negative myeloproliferative neoplasms
Most DNA methylation studies in classic Philadelphia-negative myeloproliferative neoplasms have been performed on a gene-by-gene basis. Therefore, a more comprehensive methylation profiling is needed to study the implications of this epigenetic marker in myeloproliferative neoplasms. Here, we have analyzed 71 chronic (24 polycythemia vera, 23 essential thrombocythemia and 24 primary myelofibrosis) and 13 transformed myeloproliferative neoplasms using genome-wide DNA methylation arrays. The three types of chronic Philadelphia-negative myeloproliferative neoplasms showed a similar aberrant DNA methylation pattern when compared to control samples. Differentially methylated regions were enriched in a gene network centered on the NF-κB pathway, indicating that they may be involved in the pathogenesis of these diseases. In the case of transformed myeloproliferative neoplasms, we detected an increased number of differentially methylated regions with respect to chronic myeloproliferative neoplasms. Interestingly, these genes were enriched in a list of differentially methylated regions in primary acute myeloid leukemia and in a gene network centered around the IFN pathway. Our results suggest that alterations in the DNA methylation landscape play an important role in the pathogenesis and leukemic transformation of myeloproliferative neoplasms. The therapeutic modulation of epigenetically-deregulated pathways may allow us to design targeted therapies for these patients.
0390-6078
1414-1420
Perez, C.
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Pascual, M.
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Martin-Subero, J. I.
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Bellosillo, B.
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Segura, V.
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Delabesse, E.
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Alvarez, S.
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Larrayoz, M. J.
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Rifon, J.
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Cigudosa, J. C.
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Besses, C.
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Calasanz, M. J.
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Cross, N. C. P.
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Prosper, F.
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Agirre, X.
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Perez, C.
48c10912-3582-4bc0-9f81-f0cc5856770f
Pascual, M.
33ba3cda-af3c-43c5-bb51-d41b8a1b6e58
Martin-Subero, J. I.
022b042c-dc24-44ac-8eb3-4e51658e9f90
Bellosillo, B.
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Segura, V.
ce482e38-ca49-4fa9-8d80-ae92d060cdae
Delabesse, E.
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Alvarez, S.
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Larrayoz, M. J.
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Rifon, J.
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Cigudosa, J. C.
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Besses, C.
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Calasanz, M. J.
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Cross, N. C. P.
f87650da-b908-4a34-b31b-d62c5f186fe4
Prosper, F.
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Agirre, X.
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Perez, C., Pascual, M., Martin-Subero, J. I., Bellosillo, B., Segura, V., Delabesse, E., Alvarez, S., Larrayoz, M. J., Rifon, J., Cigudosa, J. C., Besses, C., Calasanz, M. J., Cross, N. C. P., Prosper, F. and Agirre, X. (2013) Aberrant DNA methylation profile of chronic and transformed classic philadelphia-negative myeloproliferative neoplasms. Haematologica, 98 (9), 1414-1420. (doi:10.3324/haematol.2013.084160).

Record type: Article

Abstract

Most DNA methylation studies in classic Philadelphia-negative myeloproliferative neoplasms have been performed on a gene-by-gene basis. Therefore, a more comprehensive methylation profiling is needed to study the implications of this epigenetic marker in myeloproliferative neoplasms. Here, we have analyzed 71 chronic (24 polycythemia vera, 23 essential thrombocythemia and 24 primary myelofibrosis) and 13 transformed myeloproliferative neoplasms using genome-wide DNA methylation arrays. The three types of chronic Philadelphia-negative myeloproliferative neoplasms showed a similar aberrant DNA methylation pattern when compared to control samples. Differentially methylated regions were enriched in a gene network centered on the NF-κB pathway, indicating that they may be involved in the pathogenesis of these diseases. In the case of transformed myeloproliferative neoplasms, we detected an increased number of differentially methylated regions with respect to chronic myeloproliferative neoplasms. Interestingly, these genes were enriched in a list of differentially methylated regions in primary acute myeloid leukemia and in a gene network centered around the IFN pathway. Our results suggest that alterations in the DNA methylation landscape play an important role in the pathogenesis and leukemic transformation of myeloproliferative neoplasms. The therapeutic modulation of epigenetically-deregulated pathways may allow us to design targeted therapies for these patients.

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More information

Accepted/In Press date: 28 May 2013
Published date: September 2013
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 353646
URI: http://eprints.soton.ac.uk/id/eprint/353646
ISSN: 0390-6078
PURE UUID: 687d44b6-f5d5-4032-b48d-644a12ba2ada
ORCID for N. C. P. Cross: ORCID iD orcid.org/0000-0001-5481-2555

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Date deposited: 12 Jun 2013 15:14
Last modified: 22 Nov 2022 02:36

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Contributors

Author: C. Perez
Author: M. Pascual
Author: J. I. Martin-Subero
Author: B. Bellosillo
Author: V. Segura
Author: E. Delabesse
Author: S. Alvarez
Author: M. J. Larrayoz
Author: J. Rifon
Author: J. C. Cigudosa
Author: C. Besses
Author: M. J. Calasanz
Author: N. C. P. Cross ORCID iD
Author: F. Prosper
Author: X. Agirre

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