Chlamydia psittaci ewe abortion agent: complete nucleotide sequence of the major outer membrane protein gene

Pickett, M.A., Everson, J.S. and Clarke, I.N. (1988) Chlamydia psittaci ewe abortion agent: complete nucleotide sequence of the major outer membrane protein gene. FEMS Microbiology Letters, 55 (2), 229-234. (doi:10.1111/j.1574-6968.1988.tb13939.x).

Record type: Article

Abstract

The complete nucleotide sequence encoding the major outer membrane protein (MOMP) of Chlamydia psittaci strain A22/M, responsible for enzootic abortion of ewes (EAE), has been determined. An 800bp EcoRI/XbaI fragment containing a portion of the MOMP coding sequence from C. trachomatis serovar L1 was used to probe a ?L47.1 genomic library constructed from DNA obtained from C. psittaci EAE A22/M. The recombinant L47.1/EA1 was selected and contained the entire C. psittaci MOMP gene within a 7.5 kb BamHI fragment. The DNA sequence revealed an open reading frame encoding 402 amino acids, including a 22 amino acid signal peptide, which exhibited 17/22 conservation with the signal peptide of C. trachomatis MOMP. The calculated molecular mass of the C. psittaci MOMP was 43 kDa. A comparison of the MOMP genes of C. psittaci and C. trachomatis revealed only 34% nucleotide sequence homology, but 65% amino acid homology.

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