The University of Southampton
University of Southampton Institutional Repository

Chlamydia psittaci ewe abortion agent: omplete nucleotide sequence of the major outer membrane protein gene

Chlamydia psittaci ewe abortion agent: omplete nucleotide sequence of the major outer membrane protein gene
Chlamydia psittaci ewe abortion agent: omplete nucleotide sequence of the major outer membrane protein gene
The complete nucleotide sequence encoding the major outer membrane protein (MOMP) of Chlamydia psittaci strain A22/M, responsible for enzootic abortion of ewes (EAE), has been determined. An 800bp EcoRI/XbaI fragment containing a portion of the MOMP coding sequence from C. trachomatis serovar L1 was used to probe a ?L47.1 genomic library constructed from DNA obtained from C. psittaci EAE A22/M. The recombinant L47.1/EA1 was selected and contained the entire C. psittaci MOMP gene within a 7.5 kb BamHI fragment. The DNA sequence revealed an open reading frame encoding 402 amino acids, including a 22 amino acid signal peptide, which exhibited 17/22 conservation with the signal peptide of C. trachomatis MOMP. The calculated molecular mass of the C. psittaci MOMP was 43 kDa. A comparison of the MOMP genes of C. psittaci and C. trachomatis revealed only 34% nucleotide sequence homology, but 65% amino acid homology.
0378-1097
229-234
Pickett, M.A.
4b358cb6-2f2e-4691-a314-0e80df48eadb
Everson, J.S.
8f5e2cbc-b8f9-4ba6-9140-d726764d6c14
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Pickett, M.A.
4b358cb6-2f2e-4691-a314-0e80df48eadb
Everson, J.S.
8f5e2cbc-b8f9-4ba6-9140-d726764d6c14
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b

Pickett, M.A., Everson, J.S. and Clarke, I.N. (1988) Chlamydia psittaci ewe abortion agent: omplete nucleotide sequence of the major outer membrane protein gene. FEMS Microbiology Letters, 55 (2), 229-234. (doi:10.1111/j.1574-6968.1988.tb13939.x).

Record type: Article

Abstract

The complete nucleotide sequence encoding the major outer membrane protein (MOMP) of Chlamydia psittaci strain A22/M, responsible for enzootic abortion of ewes (EAE), has been determined. An 800bp EcoRI/XbaI fragment containing a portion of the MOMP coding sequence from C. trachomatis serovar L1 was used to probe a ?L47.1 genomic library constructed from DNA obtained from C. psittaci EAE A22/M. The recombinant L47.1/EA1 was selected and contained the entire C. psittaci MOMP gene within a 7.5 kb BamHI fragment. The DNA sequence revealed an open reading frame encoding 402 amino acids, including a 22 amino acid signal peptide, which exhibited 17/22 conservation with the signal peptide of C. trachomatis MOMP. The calculated molecular mass of the C. psittaci MOMP was 43 kDa. A comparison of the MOMP genes of C. psittaci and C. trachomatis revealed only 34% nucleotide sequence homology, but 65% amino acid homology.

Full text not available from this repository.

More information

Published date: 1 October 1988
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 353699
URI: https://eprints.soton.ac.uk/id/eprint/353699
ISSN: 0378-1097
PURE UUID: 0e5b7dc7-df5e-4f93-b4db-80efe5d2513e
ORCID for I.N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

Catalogue record

Date deposited: 14 Jun 2013 09:34
Last modified: 06 Jun 2018 13:19

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×