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Expression of HvHMA2 in tobacco modifies Zn–Fe–Cd homeostasis

Expression of HvHMA2 in tobacco modifies Zn–Fe–Cd homeostasis
Expression of HvHMA2 in tobacco modifies Zn–Fe–Cd homeostasis
HvHMA2 is a plasma membrane P1B-ATPase from barley that functions in Zn/Cd root-to-shoot transport. To assess the usefulness of HvHMA2 for modifying the metal content in aerial plant parts, it was expressed in tobacco under the CaMV35S promoter. Transformation with HvHMA2 did not produce one unique pattern of Zn and Cd accumulation; instead it depended on external metal supply. Thus Zn and Cd root-to-shoot translocation was facilitated, but not at all applied Zn/Cd concentrations. Metal uptake was restricted in HvHMA2-transformed plants and the level in the shoot was not enhanced. It was shown that HvHMA2 localizes to the plasma membrane of tobacco cells, and overloads the apoplast with Zn, which could explain the overall decrease in metal uptake observed. Despite the lower levels in the shoot, HvHMA2 transformants showed increased Zn sensitivity. Moreover, introduction of HvHMA2 into tobacco interfered with Fe metabolism and Fe accumulation was modified in HvHMA2-transformants in a Zn- and Cd-concentration dependent manner. The results indicate that ectopic expression of the export protein HvHMA2 in tobacco interferes with tobacco metal Zn–Cd–Fe cross-homeostasis, inducing internal mechanisms regulating metal uptake and tolerance
0176-1617
Barabasz, Anna
0e750db4-b7da-4456-8465-426452b89a2b
Wilkowska, Anna
0c4fc2be-b46e-489b-92d5-10350c3eef76
Tracz, Katarzyna
50e41a50-70ad-4a78-9d63-767a2f32131f
Ruszczyńska, Anna
3f2def84-d7ce-4fd6-b782-f8d1cf38d5a8
Bulska, Ewa
77015079-9ab5-46ef-a96b-58b6429b0387
Mills, Rebecca F.
dec4afc8-be3b-4517-9038-c3c3e8b71b35
Williams, Lorraine E.
79ee1856-3732-492b-8ac5-239749c85d9e
Antosiewicz, Danuta Maria
cbbcbcfe-2ce6-4737-9933-304d48a1590b
Barabasz, Anna
0e750db4-b7da-4456-8465-426452b89a2b
Wilkowska, Anna
0c4fc2be-b46e-489b-92d5-10350c3eef76
Tracz, Katarzyna
50e41a50-70ad-4a78-9d63-767a2f32131f
Ruszczyńska, Anna
3f2def84-d7ce-4fd6-b782-f8d1cf38d5a8
Bulska, Ewa
77015079-9ab5-46ef-a96b-58b6429b0387
Mills, Rebecca F.
dec4afc8-be3b-4517-9038-c3c3e8b71b35
Williams, Lorraine E.
79ee1856-3732-492b-8ac5-239749c85d9e
Antosiewicz, Danuta Maria
cbbcbcfe-2ce6-4737-9933-304d48a1590b

Barabasz, Anna, Wilkowska, Anna, Tracz, Katarzyna, Ruszczyńska, Anna, Bulska, Ewa, Mills, Rebecca F., Williams, Lorraine E. and Antosiewicz, Danuta Maria (2013) Expression of HvHMA2 in tobacco modifies Zn–Fe–Cd homeostasis. Journal of Plant Physiology. (doi:10.1016/j.jplph.2013.03.018). (PMID:23664582)

Record type: Article

Abstract

HvHMA2 is a plasma membrane P1B-ATPase from barley that functions in Zn/Cd root-to-shoot transport. To assess the usefulness of HvHMA2 for modifying the metal content in aerial plant parts, it was expressed in tobacco under the CaMV35S promoter. Transformation with HvHMA2 did not produce one unique pattern of Zn and Cd accumulation; instead it depended on external metal supply. Thus Zn and Cd root-to-shoot translocation was facilitated, but not at all applied Zn/Cd concentrations. Metal uptake was restricted in HvHMA2-transformed plants and the level in the shoot was not enhanced. It was shown that HvHMA2 localizes to the plasma membrane of tobacco cells, and overloads the apoplast with Zn, which could explain the overall decrease in metal uptake observed. Despite the lower levels in the shoot, HvHMA2 transformants showed increased Zn sensitivity. Moreover, introduction of HvHMA2 into tobacco interfered with Fe metabolism and Fe accumulation was modified in HvHMA2-transformants in a Zn- and Cd-concentration dependent manner. The results indicate that ectopic expression of the export protein HvHMA2 in tobacco interferes with tobacco metal Zn–Cd–Fe cross-homeostasis, inducing internal mechanisms regulating metal uptake and tolerance

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Published date: 9 May 2013
Organisations: Environmental

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Local EPrints ID: 353709
URI: https://eprints.soton.ac.uk/id/eprint/353709
ISSN: 0176-1617
PURE UUID: c5ee8760-ff1a-42b9-a486-f249275ec669

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Date deposited: 14 Jun 2013 09:01
Last modified: 18 Jul 2017 04:01

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