Accurate measurements of 13C-13C J-couplings in the rhodopsin chromophore by double-quantum solid-state NMR spectroscopy
Accurate measurements of 13C-13C J-couplings in the rhodopsin chromophore by double-quantum solid-state NMR spectroscopy
A new double-quantum solid-state NMR pulse sequence is presented and used to measure one-bond 13C-13C J-couplings in a set of 13C2-labeled rhodopsin isotopomers. The measured J-couplings reveal a perturbation of the electronic structure at the terminus of the conjugated chain but show no evidence for protein-induced electronic perturbation near the C11-C12 isomerization site. This work establishes NMR methodology for measuring accurate 1JCC values in noncrystalline macromolecules and shows that the measured J-couplings may reveal local electronic perturbations of mechanistic significance.
3878-3879
Lai, Wai Cheu
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McLean, Neville
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Gansmüller, Axel
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Verhoeven, Michiel A.
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Antonioli, Gian Carlo
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Carravetta, Marina
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Duma, Luminita
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Bovee-Geurts, Petra H.M.
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Johannessen, Ole G.
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de Groot, Hub J.M.
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Lugtenburg, Johan
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Emsley, Lyndon
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Brown, Steven P.
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Brown, Richard C.D.
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DeGrip, Willem J.
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Levitt, Malcolm H.
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29 March 2006
Lai, Wai Cheu
29df4026-12c5-4a02-a271-8e7d19c10ec0
McLean, Neville
3250113c-28d4-499b-8282-e54c3d8a9862
Gansmüller, Axel
239d731b-d3cb-4c2f-8c9e-ddb1e195c73f
Verhoeven, Michiel A.
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Antonioli, Gian Carlo
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Carravetta, Marina
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Duma, Luminita
a1547c7d-c031-4a5a-ba78-8428fab15f79
Bovee-Geurts, Petra H.M.
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Johannessen, Ole G.
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de Groot, Hub J.M.
00adea24-b494-412c-80ff-ddff45a31c45
Lugtenburg, Johan
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Emsley, Lyndon
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Brown, Steven P.
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Brown, Richard C.D.
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DeGrip, Willem J.
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Levitt, Malcolm H.
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Lai, Wai Cheu, McLean, Neville, Gansmüller, Axel, Verhoeven, Michiel A., Antonioli, Gian Carlo, Carravetta, Marina, Duma, Luminita, Bovee-Geurts, Petra H.M., Johannessen, Ole G., de Groot, Hub J.M., Lugtenburg, Johan, Emsley, Lyndon, Brown, Steven P., Brown, Richard C.D., DeGrip, Willem J. and Levitt, Malcolm H.
(2006)
Accurate measurements of 13C-13C J-couplings in the rhodopsin chromophore by double-quantum solid-state NMR spectroscopy.
Journal of the American Chemical Society, 128 (2), .
(doi:10.1021/ja0581604).
Abstract
A new double-quantum solid-state NMR pulse sequence is presented and used to measure one-bond 13C-13C J-couplings in a set of 13C2-labeled rhodopsin isotopomers. The measured J-couplings reveal a perturbation of the electronic structure at the terminus of the conjugated chain but show no evidence for protein-induced electronic perturbation near the C11-C12 isomerization site. This work establishes NMR methodology for measuring accurate 1JCC values in noncrystalline macromolecules and shows that the measured J-couplings may reveal local electronic perturbations of mechanistic significance.
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Published date: 29 March 2006
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Local EPrints ID: 35407
URI: http://eprints.soton.ac.uk/id/eprint/35407
ISSN: 0002-7863
PURE UUID: ae9d732f-9eb2-46f7-952f-587b984efce6
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Date deposited: 16 May 2006
Last modified: 16 Mar 2024 03:29
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Contributors
Author:
Wai Cheu Lai
Author:
Neville McLean
Author:
Axel Gansmüller
Author:
Michiel A. Verhoeven
Author:
Gian Carlo Antonioli
Author:
Luminita Duma
Author:
Petra H.M. Bovee-Geurts
Author:
Ole G. Johannessen
Author:
Hub J.M. de Groot
Author:
Johan Lugtenburg
Author:
Lyndon Emsley
Author:
Steven P. Brown
Author:
Willem J. DeGrip
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