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Accurate measurements of 13C-13C J-couplings in the rhodopsin chromophore by double-quantum solid-state NMR spectroscopy

Accurate measurements of 13C-13C J-couplings in the rhodopsin chromophore by double-quantum solid-state NMR spectroscopy
Accurate measurements of 13C-13C J-couplings in the rhodopsin chromophore by double-quantum solid-state NMR spectroscopy
A new double-quantum solid-state NMR pulse sequence is presented and used to measure one-bond 13C-13C J-couplings in a set of 13C2-labeled rhodopsin isotopomers. The measured J-couplings reveal a perturbation of the electronic structure at the terminus of the conjugated chain but show no evidence for protein-induced electronic perturbation near the C11-C12 isomerization site. This work establishes NMR methodology for measuring accurate 1JCC values in noncrystalline macromolecules and shows that the measured J-couplings may reveal local electronic perturbations of mechanistic significance.
0002-7863
3878-3879
Lai, Wai Cheu
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McLean, Neville
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Gansmüller, Axel
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Verhoeven, Michiel A.
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Antonioli, Gian Carlo
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Carravetta, Marina
1b12fa96-4a6a-4689-ab3b-ccc68f1d7691
Duma, Luminita
a1547c7d-c031-4a5a-ba78-8428fab15f79
Bovee-Geurts, Petra H.M.
66d6099e-73b5-4f84-ae7a-2b61d874e4e9
Johannessen, Ole G.
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de Groot, Hub J.M.
00adea24-b494-412c-80ff-ddff45a31c45
Lugtenburg, Johan
b920f5ab-0138-479f-88b1-f4bca6b32dad
Emsley, Lyndon
3234816a-24e9-44a0-b134-70c09de78257
Brown, Steven P.
ecbc78d6-023f-41d3-8e84-bd8c7fbfcbfa
Brown, Richard C.D.
21ce697a-7c3a-480e-919f-429a3d8550f5
DeGrip, Willem J.
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Levitt, Malcolm H.
bcc5a80a-e5c5-4e0e-9a9a-249d036747c3
Lai, Wai Cheu
29df4026-12c5-4a02-a271-8e7d19c10ec0
McLean, Neville
3250113c-28d4-499b-8282-e54c3d8a9862
Gansmüller, Axel
67c127f1-7776-436e-b195-5e137c47fbce
Verhoeven, Michiel A.
94b2c838-a88e-4388-8f2c-3788dbcb1f76
Antonioli, Gian Carlo
999af89b-f81c-4680-897a-e2657282b3ae
Carravetta, Marina
1b12fa96-4a6a-4689-ab3b-ccc68f1d7691
Duma, Luminita
a1547c7d-c031-4a5a-ba78-8428fab15f79
Bovee-Geurts, Petra H.M.
66d6099e-73b5-4f84-ae7a-2b61d874e4e9
Johannessen, Ole G.
799ccc8c-a2e7-4305-a03a-2dc9f42564ef
de Groot, Hub J.M.
00adea24-b494-412c-80ff-ddff45a31c45
Lugtenburg, Johan
b920f5ab-0138-479f-88b1-f4bca6b32dad
Emsley, Lyndon
3234816a-24e9-44a0-b134-70c09de78257
Brown, Steven P.
ecbc78d6-023f-41d3-8e84-bd8c7fbfcbfa
Brown, Richard C.D.
21ce697a-7c3a-480e-919f-429a3d8550f5
DeGrip, Willem J.
3fe658dc-e4cd-4d05-9771-417fd462ae72
Levitt, Malcolm H.
bcc5a80a-e5c5-4e0e-9a9a-249d036747c3

Lai, Wai Cheu, McLean, Neville, Gansmüller, Axel, Verhoeven, Michiel A., Antonioli, Gian Carlo, Carravetta, Marina, Duma, Luminita, Bovee-Geurts, Petra H.M., Johannessen, Ole G., de Groot, Hub J.M., Lugtenburg, Johan, Emsley, Lyndon, Brown, Steven P., Brown, Richard C.D., DeGrip, Willem J. and Levitt, Malcolm H. (2006) Accurate measurements of 13C-13C J-couplings in the rhodopsin chromophore by double-quantum solid-state NMR spectroscopy. Journal of the American Chemical Society, 128 (2), 3878-3879. (doi:10.1021/ja0581604).

Record type: Article

Abstract

A new double-quantum solid-state NMR pulse sequence is presented and used to measure one-bond 13C-13C J-couplings in a set of 13C2-labeled rhodopsin isotopomers. The measured J-couplings reveal a perturbation of the electronic structure at the terminus of the conjugated chain but show no evidence for protein-induced electronic perturbation near the C11-C12 isomerization site. This work establishes NMR methodology for measuring accurate 1JCC values in noncrystalline macromolecules and shows that the measured J-couplings may reveal local electronic perturbations of mechanistic significance.

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Published date: 29 March 2006

Identifiers

Local EPrints ID: 35407
URI: http://eprints.soton.ac.uk/id/eprint/35407
ISSN: 0002-7863
PURE UUID: ae9d732f-9eb2-46f7-952f-587b984efce6
ORCID for Marina Carravetta: ORCID iD orcid.org/0000-0002-6296-2104
ORCID for Richard C.D. Brown: ORCID iD orcid.org/0000-0003-0156-7087
ORCID for Malcolm H. Levitt: ORCID iD orcid.org/0000-0001-9878-1180

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Date deposited: 16 May 2006
Last modified: 03 Dec 2019 01:59

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Contributors

Author: Wai Cheu Lai
Author: Neville McLean
Author: Axel Gansmüller
Author: Michiel A. Verhoeven
Author: Gian Carlo Antonioli
Author: Luminita Duma
Author: Petra H.M. Bovee-Geurts
Author: Ole G. Johannessen
Author: Hub J.M. de Groot
Author: Johan Lugtenburg
Author: Lyndon Emsley
Author: Steven P. Brown
Author: Willem J. DeGrip

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