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Programmable large area digital microfluidic array with integrated droplet sensing for bioassays

Programmable large area digital microfluidic array with integrated droplet sensing for bioassays
Programmable large area digital microfluidic array with integrated droplet sensing for bioassays
We describe a new device concept for digital microfluidics, based on an active matrix electrowetting on dielectric (AM-EWOD) device. A conventional EWOD device is limited by the number of electrical connections that can be made practically, which restricts the number and type of droplet operations. In an AM-EWOD, the patterned electrodes of a conventional EWOD device are replaced by a thin film transistor (TFT) array, as found in a liquid crystal display (LCD), facilitating independent control of each electrode. The arrays can have many thousand individually addressable electrodes, are fully reconfigurable and can be programmed to support multiple simultaneous operations. Each element is 210 µm × 210 µm in size and contains a circuit that measures the electrical impedance of the liquid above it. This is used to determine the presence and size of a droplet, a method that can improve assay reliability and accuracy. This sensor provides feedback, error detection and closed loop control of an assay sequence. We describe the design, fabrication and testing of a 64 × 64 format AM-EWOD device with impedance sensor functionality. A colorimetric assay is implemented on the device and used to measure glucose in human blood serum. Results are compared with the same assay performed on a microtitre plate.



1473-0197
3305-3313
Hadwen, B.
b58638f7-4397-41cf-b5ef-1470a0cdf430
Broder, G. R.
8d7bd385-bf04-4970-bbd9-e4c76462491f
Morganti, D.
c8e436d0-2fa0-4061-88dd-ca903928a080
Jacobs, A.
1c8942a6-c743-42d0-90e3-945825410c4b
Brown, C.
c49e4fb2-21c3-4e8c-90b3-e780296c0919
Hector, J. R.
190675af-2b8f-475b-9994-8e44a162ca73
Kubota, Y.
8697b6f6-a9bf-4413-a8d4-58ca23f8f768
Morgan, H.
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Hadwen, B.
b58638f7-4397-41cf-b5ef-1470a0cdf430
Broder, G. R.
8d7bd385-bf04-4970-bbd9-e4c76462491f
Morganti, D.
c8e436d0-2fa0-4061-88dd-ca903928a080
Jacobs, A.
1c8942a6-c743-42d0-90e3-945825410c4b
Brown, C.
c49e4fb2-21c3-4e8c-90b3-e780296c0919
Hector, J. R.
190675af-2b8f-475b-9994-8e44a162ca73
Kubota, Y.
8697b6f6-a9bf-4413-a8d4-58ca23f8f768
Morgan, H.
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Hadwen, B., Broder, G. R., Morganti, D., Jacobs, A., Brown, C., Hector, J. R., Kubota, Y. and Morgan, H. (2012) Programmable large area digital microfluidic array with integrated droplet sensing for bioassays. Lab on a Chip, 12 (18), 3305-3313. (doi:10.1039/C2LC40273D). (PMID:22785575)

Record type: Article

Abstract

We describe a new device concept for digital microfluidics, based on an active matrix electrowetting on dielectric (AM-EWOD) device. A conventional EWOD device is limited by the number of electrical connections that can be made practically, which restricts the number and type of droplet operations. In an AM-EWOD, the patterned electrodes of a conventional EWOD device are replaced by a thin film transistor (TFT) array, as found in a liquid crystal display (LCD), facilitating independent control of each electrode. The arrays can have many thousand individually addressable electrodes, are fully reconfigurable and can be programmed to support multiple simultaneous operations. Each element is 210 µm × 210 µm in size and contains a circuit that measures the electrical impedance of the liquid above it. This is used to determine the presence and size of a droplet, a method that can improve assay reliability and accuracy. This sensor provides feedback, error detection and closed loop control of an assay sequence. We describe the design, fabrication and testing of a 64 × 64 format AM-EWOD device with impedance sensor functionality. A colorimetric assay is implemented on the device and used to measure glucose in human blood serum. Results are compared with the same assay performed on a microtitre plate.



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More information

Published date: 25 May 2012
Organisations: Electronics & Computer Science

Identifiers

Local EPrints ID: 354476
URI: https://eprints.soton.ac.uk/id/eprint/354476
ISSN: 1473-0197
PURE UUID: 378008d1-5d10-44a1-82da-7b2e4526d798
ORCID for H. Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 15 Jul 2013 12:39
Last modified: 29 Aug 2019 00:44

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Contributors

Author: B. Hadwen
Author: G. R. Broder
Author: D. Morganti
Author: A. Jacobs
Author: C. Brown
Author: J. R. Hector
Author: Y. Kubota
Author: H. Morgan ORCID iD

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