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Microfluidic lysis of human blood for leukocyte analysis using single cell impedance cytometry

Microfluidic lysis of human blood for leukocyte analysis using single cell impedance cytometry
Microfluidic lysis of human blood for leukocyte analysis using single cell impedance cytometry
This paper demonstrates an integrated microfluidic system that performs a full blood count using impedance analysis. A microfluidic network design for red blood cell (RBC) lysis is presented, and the diffusive mixing processes are analyzed using experimental and simulated results. Healthy and clinical bloods analyzed with this system, and the data shows good correlation against data obtained from commercial hematology machines. The data from the microfluidic system was compared against hospital data for 18 clinical samples, giving R2 (coefficient of determination) values of 0.99 for lymphocytes, 0.89 for monocytes, and 0.99 for granulocytes in terms of relative counts and 0.94 for lymphocytes, 0.91 for monocytes, and 0.95 for granulocytes in terms of absolute counts. This demonstrates the potential clinical utility of this new system for a point-of-care purpose.
0003-2700
1070-1075
Han, Xiaojun
909e2893-2df4-41c7-85aa-9b23df5d130c
van Berkel, Cees
148b8dd1-6357-4308-81af-1ee11d287e99
Gwyer, James
7bfb38d9-b963-4b18-a532-5da232f73835
Capretto, Lorenzo
0f3586b5-1560-49c1-a76b-59e74ea600ef
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Han, Xiaojun
909e2893-2df4-41c7-85aa-9b23df5d130c
van Berkel, Cees
148b8dd1-6357-4308-81af-1ee11d287e99
Gwyer, James
7bfb38d9-b963-4b18-a532-5da232f73835
Capretto, Lorenzo
0f3586b5-1560-49c1-a76b-59e74ea600ef
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Han, Xiaojun, van Berkel, Cees, Gwyer, James, Capretto, Lorenzo and Morgan, Hywel (2011) Microfluidic lysis of human blood for leukocyte analysis using single cell impedance cytometry. Analytical Chemistry, 84 (2), 1070-1075. (doi:10.1021/ac202700x).

Record type: Article

Abstract

This paper demonstrates an integrated microfluidic system that performs a full blood count using impedance analysis. A microfluidic network design for red blood cell (RBC) lysis is presented, and the diffusive mixing processes are analyzed using experimental and simulated results. Healthy and clinical bloods analyzed with this system, and the data shows good correlation against data obtained from commercial hematology machines. The data from the microfluidic system was compared against hospital data for 18 clinical samples, giving R2 (coefficient of determination) values of 0.99 for lymphocytes, 0.89 for monocytes, and 0.99 for granulocytes in terms of relative counts and 0.94 for lymphocytes, 0.91 for monocytes, and 0.95 for granulocytes in terms of absolute counts. This demonstrates the potential clinical utility of this new system for a point-of-care purpose.

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More information

Published date: 9 December 2011
Organisations: Electronics & Computer Science

Identifiers

Local EPrints ID: 354598
URI: https://eprints.soton.ac.uk/id/eprint/354598
ISSN: 0003-2700
PURE UUID: 36166648-7b0b-4ce1-8309-7d29a360ffbf
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 16 Jul 2013 10:16
Last modified: 17 Sep 2019 00:52

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