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Parallel recording of single ion channels: a heterogeneous system approach

Parallel recording of single ion channels: a heterogeneous system approach
Parallel recording of single ion channels: a heterogeneous system approach
The convergence of integrated electronic devices with nanotechnology structures on heterogeneous systems presents promising opportunities for the development of new classes of rapid, sensitive, and reliable sensors. The main advantage of embedding microelectronic readout structures with sensing elements is twofold. On the one hand, the SNR is increased as a result of scaling. On the other, readout miniaturization allows organization of sensors into arrays. The latter point will improve sensing accuracy by using statistical methods. However, accurate interface design is required to establish efficient communication between ionic-based and electronic-based signals. This paper shows a first example of a concurrent readout system with single-ion channel resolution, using a compact and scalable architecture. An array of biological nanosensors is organized on different layers stacked together in a mixed structure: fluidics, printed circuit board, and microelectronic readout. More specifically, an array of microholes machined into a polyoxymethylene homopolymer (POMH or Delrin) device coupled with ultralow noise sigma-delta converters current amplifiers, is used to form bilayer membranes within which ion channels are embedded. It is shown how formation of multiple artificial bilayer lipid membranes (BLMs) is automatically monitored by the interface. The system is used to detect current signals in the pA range, from noncovalent binding between single, BLM-embedded ??-hemolysin pores and ??-cyclodextrin molecules. The current signals are concurrently processed by the readout structure.
bilayer lipid membrane (blm), ion channels, nanosensors, sigma-delta converters
295-302
Thei, Federico
c5014b97-007e-482d-87ad-1d43162ac2eb
Rossi, Michele
e09fb28f-913c-4204-b350-e462d2f6440f
Bennati, Marco
dd4ae84d-21e3-414b-a8c1-c4abbbf12c3e
Crescentini, Marco
568e8046-74bd-449f-8794-889643147059
Lodesani, Francesco
a4da1c00-2917-43ac-bbe9-27b7270bbc59
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Tartagni, Marco
665c139f-e76b-49e2-8cf7-199478891464
Thei, Federico
c5014b97-007e-482d-87ad-1d43162ac2eb
Rossi, Michele
e09fb28f-913c-4204-b350-e462d2f6440f
Bennati, Marco
dd4ae84d-21e3-414b-a8c1-c4abbbf12c3e
Crescentini, Marco
568e8046-74bd-449f-8794-889643147059
Lodesani, Francesco
a4da1c00-2917-43ac-bbe9-27b7270bbc59
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Tartagni, Marco
665c139f-e76b-49e2-8cf7-199478891464

Thei, Federico, Rossi, Michele, Bennati, Marco, Crescentini, Marco, Lodesani, Francesco, Morgan, Hywel and Tartagni, Marco (2010) Parallel recording of single ion channels: a heterogeneous system approach. IEEE Transactions on Nanotechnology, 9 (3), 295-302. (doi:10.1109/TNANO.2009.2039489).

Record type: Article

Abstract

The convergence of integrated electronic devices with nanotechnology structures on heterogeneous systems presents promising opportunities for the development of new classes of rapid, sensitive, and reliable sensors. The main advantage of embedding microelectronic readout structures with sensing elements is twofold. On the one hand, the SNR is increased as a result of scaling. On the other, readout miniaturization allows organization of sensors into arrays. The latter point will improve sensing accuracy by using statistical methods. However, accurate interface design is required to establish efficient communication between ionic-based and electronic-based signals. This paper shows a first example of a concurrent readout system with single-ion channel resolution, using a compact and scalable architecture. An array of biological nanosensors is organized on different layers stacked together in a mixed structure: fluidics, printed circuit board, and microelectronic readout. More specifically, an array of microholes machined into a polyoxymethylene homopolymer (POMH or Delrin) device coupled with ultralow noise sigma-delta converters current amplifiers, is used to form bilayer membranes within which ion channels are embedded. It is shown how formation of multiple artificial bilayer lipid membranes (BLMs) is automatically monitored by the interface. The system is used to detect current signals in the pA range, from noncovalent binding between single, BLM-embedded ??-hemolysin pores and ??-cyclodextrin molecules. The current signals are concurrently processed by the readout structure.

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More information

Published date: 13 May 2010
Keywords: bilayer lipid membrane (blm), ion channels, nanosensors, sigma-delta converters
Organisations: Electronics & Computer Science

Identifiers

Local EPrints ID: 355152
URI: https://eprints.soton.ac.uk/id/eprint/355152
PURE UUID: 239e9029-d6c2-40d7-a89e-e5b1d107115b
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 13 Aug 2013 10:26
Last modified: 20 Jul 2019 00:59

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