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Continuous differential impedance spectroscopy of single cells

Continuous differential impedance spectroscopy of single cells
Continuous differential impedance spectroscopy of single cells
A device for continuous differential impedance analysis of single cells held by a hydrodynamic cell trapping is presented. Measurements are accomplished by recording the current from two closely-situated electrode pairs, one empty (reference) and one containing a cell. We demonstrate time-dependent measurement of single cell impedance produced in response to dynamic chemical perturbations. First, the system is used to assay the response of HeLa cells to the effects of the surfactant Tween, which reduces the impedance of the trapped cells in a concentration dependent way and is interpreted as gradual lysis of the cell membrane. Second, the effects of the bacterial pore-forming toxin, Streptolysin-O are measured: a transient exponential decay in the impedance is recorded as the cell membrane becomes increasingly permeable. The decay time constant is inversely proportional to toxin concentration (482, 150, and 30 s for 0.1, 1, and 10 kU/ml, respectively).
impedance spectroscopy, single-cell analysis, microfluidic, microfabrication
1613-4982
191-198
Malleo, Daniele
d446665d-833c-47db-8a6f-ebaf172df477
Nevill, J. Tanner
506f8edd-e660-4b8e-8f5e-24a71a1c6f3c
Lee, Luke P.
d9c8ccb5-5adf-44f9-945b-ee011db66a7d
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Malleo, Daniele
d446665d-833c-47db-8a6f-ebaf172df477
Nevill, J. Tanner
506f8edd-e660-4b8e-8f5e-24a71a1c6f3c
Lee, Luke P.
d9c8ccb5-5adf-44f9-945b-ee011db66a7d
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Malleo, Daniele, Nevill, J. Tanner, Lee, Luke P. and Morgan, Hywel (2010) Continuous differential impedance spectroscopy of single cells. Microfluidics and Nanofluidics, 9 (2-3), 191-198. (doi:10.1007/s10404-009-0534-2).

Record type: Article

Abstract

A device for continuous differential impedance analysis of single cells held by a hydrodynamic cell trapping is presented. Measurements are accomplished by recording the current from two closely-situated electrode pairs, one empty (reference) and one containing a cell. We demonstrate time-dependent measurement of single cell impedance produced in response to dynamic chemical perturbations. First, the system is used to assay the response of HeLa cells to the effects of the surfactant Tween, which reduces the impedance of the trapped cells in a concentration dependent way and is interpreted as gradual lysis of the cell membrane. Second, the effects of the bacterial pore-forming toxin, Streptolysin-O are measured: a transient exponential decay in the impedance is recorded as the cell membrane becomes increasingly permeable. The decay time constant is inversely proportional to toxin concentration (482, 150, and 30 s for 0.1, 1, and 10 kU/ml, respectively).

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More information

Published date: 9 August 2010
Keywords: impedance spectroscopy, single-cell analysis, microfluidic, microfabrication
Organisations: Electronics & Computer Science

Identifiers

Local EPrints ID: 355158
URI: https://eprints.soton.ac.uk/id/eprint/355158
ISSN: 1613-4982
PURE UUID: 4437078f-77d4-4e4f-b2a2-97365b12dd2b
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 13 Aug 2013 10:36
Last modified: 29 Aug 2019 00:44

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Contributors

Author: Daniele Malleo
Author: J. Tanner Nevill
Author: Luke P. Lee
Author: Hywel Morgan ORCID iD

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