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The monosaccharide transporter gene, AtSTP4, and the cell-wall invertase, At beta fruct1, are induced in Arabidopsis during infection with the fungal biotroph Erysiphe cichoracearum

The monosaccharide transporter gene, AtSTP4, and the cell-wall invertase, At beta fruct1, are induced in Arabidopsis during infection with the fungal biotroph Erysiphe cichoracearum
The monosaccharide transporter gene, AtSTP4, and the cell-wall invertase, At beta fruct1, are induced in Arabidopsis during infection with the fungal biotroph Erysiphe cichoracearum
Powdery mildew fungi are biotrophic pathogens that form a complex interface, the haustorium, between the host plant and the parasite. The pathogen acts as an additional sink, competing with host sinks, resulting in considerable modification of photoassimilate production and partitioning within the host tissue. Here, we examine the factors that may contribute to these changes. We show for the first time in one biotrophic interaction (Arabidopsis/Erysiphe cichoracearum) all of the following responses: Glc uptake in host tissues is enhanced after fungal infection; this coincides with the induction of expression of the monosaccharide transporter gene, Arabidopsis sugar transport protein 4 (AtSTP4), in infected leaves; invertase activity and transcript levels for a cell wall invertase, Atfruct1, increase substantially in Arabidopsis during attack by this pathogen. Before infection, Arabidopsis plants transformed with an AtSTP4 promoter--glucuronidase construct show expression mainly in sink tissues such as roots; after infection, AtSTP4 expression is induced in the mature leaves and increases over the 6-d time period. Sections of infected leaves stained for -glucuronidase show that AtSTP4 expression is not confined to infected epidermal cells but is also evident in a wider range of cells, including those of the vascular tissue. The results are discussed in relation to the possible coordinated expression of hexose transporters and cell wall invertase in the host response to powdery mildew infection.
0032-0889
821-829
Fotopoulos, Vasileios
f3a676b1-2a42-4093-b35c-da263bc90fbb
Gilbert, Martin J.
981d65ca-9669-49bf-b5ab-d568d36058d6
Pittman, Jon K.
186ccb17-c717-43d5-b583-293c7cc986ea
Marvier, Alison C.
d8e189c6-c01d-472b-afe0-bb8ad0b88eb1
Buchanan, Aram J.
4436fa28-ac08-4910-9103-59f3e8cd25d7
Sauer, Norbert
155ffe69-fc5b-4f21-af99-32121f3c2a56
Hall, J.L.
24cd62e9-4050-4514-9446-5a03949007f4
Williams, Lorraine E.
79ee1856-3732-492b-8ac5-239749c85d9e
Fotopoulos, Vasileios
f3a676b1-2a42-4093-b35c-da263bc90fbb
Gilbert, Martin J.
981d65ca-9669-49bf-b5ab-d568d36058d6
Pittman, Jon K.
186ccb17-c717-43d5-b583-293c7cc986ea
Marvier, Alison C.
d8e189c6-c01d-472b-afe0-bb8ad0b88eb1
Buchanan, Aram J.
4436fa28-ac08-4910-9103-59f3e8cd25d7
Sauer, Norbert
155ffe69-fc5b-4f21-af99-32121f3c2a56
Hall, J.L.
24cd62e9-4050-4514-9446-5a03949007f4
Williams, Lorraine E.
79ee1856-3732-492b-8ac5-239749c85d9e

Fotopoulos, Vasileios, Gilbert, Martin J., Pittman, Jon K., Marvier, Alison C., Buchanan, Aram J., Sauer, Norbert, Hall, J.L. and Williams, Lorraine E. (2003) The monosaccharide transporter gene, AtSTP4, and the cell-wall invertase, At beta fruct1, are induced in Arabidopsis during infection with the fungal biotroph Erysiphe cichoracearum. Plant Physiology, 132 (2), 821-829. (doi:10.1104/pp.103.021428).

Record type: Article

Abstract

Powdery mildew fungi are biotrophic pathogens that form a complex interface, the haustorium, between the host plant and the parasite. The pathogen acts as an additional sink, competing with host sinks, resulting in considerable modification of photoassimilate production and partitioning within the host tissue. Here, we examine the factors that may contribute to these changes. We show for the first time in one biotrophic interaction (Arabidopsis/Erysiphe cichoracearum) all of the following responses: Glc uptake in host tissues is enhanced after fungal infection; this coincides with the induction of expression of the monosaccharide transporter gene, Arabidopsis sugar transport protein 4 (AtSTP4), in infected leaves; invertase activity and transcript levels for a cell wall invertase, Atfruct1, increase substantially in Arabidopsis during attack by this pathogen. Before infection, Arabidopsis plants transformed with an AtSTP4 promoter--glucuronidase construct show expression mainly in sink tissues such as roots; after infection, AtSTP4 expression is induced in the mature leaves and increases over the 6-d time period. Sections of infected leaves stained for -glucuronidase show that AtSTP4 expression is not confined to infected epidermal cells but is also evident in a wider range of cells, including those of the vascular tissue. The results are discussed in relation to the possible coordinated expression of hexose transporters and cell wall invertase in the host response to powdery mildew infection.

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Published date: June 2003
Organisations: Biological Sciences

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Local EPrints ID: 35516
URI: http://eprints.soton.ac.uk/id/eprint/35516
ISSN: 0032-0889
PURE UUID: 60c55202-9a02-4fba-baf2-5f28f6b142b9

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Date deposited: 17 May 2006
Last modified: 15 Mar 2024 07:52

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Contributors

Author: Vasileios Fotopoulos
Author: Martin J. Gilbert
Author: Jon K. Pittman
Author: Alison C. Marvier
Author: Aram J. Buchanan
Author: Norbert Sauer
Author: J.L. Hall

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