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The 1.6 Angstrom X-ray structure of the unusual c-type cytochrome, cytochrome c(L), from the methylotrophic bacterium Methylobacterium extorquens

Record type: Article

The structure of cytochrome c(L) from Methylobacterium extorquens has been determined by X-ray crystallography to a resolution of 1.6 Angstrom. This unusually large, acidic cytochrome is the physiological electron acceptor for the quinoprotein methanol dehydrogenase in the periplasm of methylotrophic bacteria. Its amino acid sequence is completely different from that of other cytochromes but its X-ray structure reveals a core that is typical of class I cytochromes c, having alpha-helices folded into a compact, structure enclosing the single haem c prosthetic group and leaving one edge of the haem exposed. The haem is bound through thioether bonds to Cys65 and Cys68, and the fifth ligand to the haem iron is provided by, His69. Remarkably, the sixth ligand is provided by His112, and not by ligand in solution. Met109, which had been shown to be the sixth ligand in solution. Cytochrome c(L) is unusual in having a disulphide bridge that tethers the long C-terminal extension to the body of the structure. The crystal structure reveals that, close to the inner haem propionate, there is tightly bound calcium ion that is likely to be involved in stabilization of the redox potential, and that may be important in the flow of electrons from reduced pyrroloquinoline quinone in methanol dehydrogenase to the haem of cytochrome c(L). As predicted, both haem propionates are exposed to solvent, accounting for the unusual influence of pH on the redox potential of this cytochrome.

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Citation

Williams, P., Coates, L., Mohammed, F., Gill, R., Erskine, P., Bourgeois, D., Wood, S.P., Anthony, C. and Cooper, J.B. (2006) The 1.6 Angstrom X-ray structure of the unusual c-type cytochrome, cytochrome c(L), from the methylotrophic bacterium Methylobacterium extorquens Journal of Molecular Biology, 357, (1), pp. 151-162. (doi:10.1016/j.jmb.2005.12.055).

More information

Published date: 2006
Keywords: cytochrome c(L), crystal structure, electron transport, methylotroph, Methylobacterium extorquens

Identifiers

Local EPrints ID: 35537
URI: http://eprints.soton.ac.uk/id/eprint/35537
ISSN: 0022-2836
PURE UUID: baa7e293-ddf1-4166-bf4f-71582469be7c

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Date deposited: 18 May 2006
Last modified: 17 Jul 2017 15:47

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Contributors

Author: P. Williams
Author: L. Coates
Author: F. Mohammed
Author: R. Gill
Author: P. Erskine
Author: D. Bourgeois
Author: S.P. Wood
Author: C. Anthony
Author: J.B. Cooper

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