The plant P1B-type ATPase AtHMA4 transports Zn and Cd and plays a role in detoxification of transition metals supplied at elevated levels


Millls, R.F., Francini, A., Ferreira da Rocha, P.S.C., Baccarini, P.J., Aylett, M., Krijger, G.C. and Williams, L.E. (2005) The plant P1B-type ATPase AtHMA4 transports Zn and Cd and plays a role in detoxification of transition metals supplied at elevated levels Febs Letters, 579, (3), pp. 783-791. (doi:10.1016/j.febslet.2004.12.040).

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Description/Abstract

The transition metal Zn is essential for many physiological processes in plants, yet at elevated concentrations this, and the related non-essential metal Cd, can be toxic. Arabidopsis thaliana HMA4, belonging to the Type P1B subfamily of P-type ATPases, has recently been implicated in Zn nutrition, having a role in root to shoot Zn translocation. Using Arabidopsis insertional mutants, it is shown here that disruption of AtHMA4 function also results in increased sensitivity to elevated levels of Cd and Zn, suggesting that AtHMA4 serves an important role in metal detoxification at higher metal concentrations. AtHMA4 and a truncated form lacking the last 457 amino acids both confer Cd and Zn resistance to yeast but a mutant version of the full-length AtHMA4 (AtHMA4-C357G) does not; this demonstrates that the C-terminal region is not essential for this function. Evidence is presented that AtHMA4 functions as an efflux pump.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1016/j.febslet.2004.12.040
ISSNs: 0014-5793 (print)
Subjects:
ePrint ID: 35538
Date :
Date Event
3 August 2004Submitted
27 December 2005Published
Date Deposited: 18 May 2006
Last Modified: 16 Apr 2017 22:09
Further Information:Google Scholar
URI: http://eprints.soton.ac.uk/id/eprint/35538

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