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The plant P-1B-type ATPase AtHMA4 transports Zn and Cd and plays a role in detoxification of transition metals supplied at elevated levels

Record type: Article

The transition metal Zn is essential for many physiological processes in plants, yet at elevated concentrations this, and the related non-essential metal Cd, can be toxic. Arabidopsis thaliana HMA4, belonging to the Type P1B subfamily of P-type ATPases, has recently been implicated in Zn nutrition, having a role in root to shoot Zn translocation. Using Arabidopsis insertional mutants, it is shown here that disruption of AtHMA4 function also results in increased sensitivity to elevated levels of Cd and Zn, suggesting that AtHMA4 serves an important role in metal detoxification at higher metal concentrations. AtHMA4 and a truncated form lacking the last 457 amino acids both confer Cd and Zn resistance to yeast but a mutant version of the full-length AtHMA4 (AtHMA4-C357G) does not; this demonstrates that the C-terminal region is not essential for this function. Evidence is presented that AtHMA4 functions as an efflux pump.

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Citation

Mills, Rebecca F., Francini, Alessandra, Ferreira da Rocha, Pedro S.C., Baccarini, Paul J., Aylett, Melissa, Krijger, Gerard C. and Williams, Lorraine E. (2005) The plant P-1B-type ATPase AtHMA4 transports Zn and Cd and plays a role in detoxification of transition metals supplied at elevated levels FEBS Letters, 579, (3), pp. 783-791. (doi:10.1016/j.febslet.2004.12.040).

More information

Submitted date: 3 August 2004
Published date: 31 January 2005
Keywords: heterologous yeast expression, metal homeostasis, insertional mutant, P-type ATPase, transition metal transport, arabidopsis thaliana
Organisations: Biological Sciences

Identifiers

Local EPrints ID: 35538
URI: http://eprints.soton.ac.uk/id/eprint/35538
ISSN: 0014-5793
PURE UUID: 01d7ccb0-b300-4c4f-abc6-f4b1e36170b1

Catalogue record

Date deposited: 18 May 2006
Last modified: 17 Jul 2017 15:47

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Contributors

Author: Rebecca F. Mills
Author: Alessandra Francini
Author: Pedro S.C. Ferreira da Rocha
Author: Paul J. Baccarini
Author: Melissa Aylett
Author: Gerard C. Krijger

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