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Dielectrophoretic investigation of plant virus particles: Cow Pea Mosaic Virus and Tobacco Mosaic Virus

Dielectrophoretic investigation of plant virus particles: Cow Pea Mosaic Virus and Tobacco Mosaic Virus
Dielectrophoretic investigation of plant virus particles: Cow Pea Mosaic Virus and Tobacco Mosaic Virus
This paper reports experimental results on the dielectrophoretic (DEP) behaviour on two nonenveloped plant viruses of different geometrical shapes, namely Cow Pea Mosaic Virus (CPMV) and Tobacco Mosaic Virus (TMV). The DEP properties of carboxy-modified latex beads of the same size are also reported. The DEP properties of single particles were obtained from measurement of the frequency at which the DEP force on a particle goes to zero (the crossover frequency). The DEP behaviour of particle ensembles was also measured using image processing. The dielectric properties of the particles were evaluated from the DEP data. The surface conductance was found to be 0.3 nS for CPMV, 0.38 nS for TMV, and 0.52 nS for 27 nm diameter carboxy-latex beads. Data analysis has shown that the optimal condition for separation of TMV and CPMV is a low-conductivity suspending medium - below 1 mS/m.
AC electrokinetics, dielectrophoresis, latex beads, surface conductance, virus particles
0173-0835
3939-3948
Ermolina, Irina
92926818-1179-4335-b7e5-9970cf542807
Milner, Joel
a9271fbb-fa45-4eb9-8c21-fb625f3741a0
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Ermolina, Irina
92926818-1179-4335-b7e5-9970cf542807
Milner, Joel
a9271fbb-fa45-4eb9-8c21-fb625f3741a0
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Ermolina, Irina, Milner, Joel and Morgan, Hywel (2006) Dielectrophoretic investigation of plant virus particles: Cow Pea Mosaic Virus and Tobacco Mosaic Virus. Electrophoresis, 27 (20), 3939-3948. (doi:10.1002/elps.200500928). (PMID:17054097)

Record type: Article

Abstract

This paper reports experimental results on the dielectrophoretic (DEP) behaviour on two nonenveloped plant viruses of different geometrical shapes, namely Cow Pea Mosaic Virus (CPMV) and Tobacco Mosaic Virus (TMV). The DEP properties of carboxy-modified latex beads of the same size are also reported. The DEP properties of single particles were obtained from measurement of the frequency at which the DEP force on a particle goes to zero (the crossover frequency). The DEP behaviour of particle ensembles was also measured using image processing. The dielectric properties of the particles were evaluated from the DEP data. The surface conductance was found to be 0.3 nS for CPMV, 0.38 nS for TMV, and 0.52 nS for 27 nm diameter carboxy-latex beads. Data analysis has shown that the optimal condition for separation of TMV and CPMV is a low-conductivity suspending medium - below 1 mS/m.

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More information

Published date: October 2006
Keywords: AC electrokinetics, dielectrophoresis, latex beads, surface conductance, virus particles
Organisations: Electronics & Computer Science

Identifiers

Local EPrints ID: 355526
URI: https://eprints.soton.ac.uk/id/eprint/355526
ISSN: 0173-0835
PURE UUID: 2ece4c84-caf6-42ab-ba49-eac745862685
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 22 Oct 2013 11:19
Last modified: 10 Sep 2019 00:45

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