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High speed simultaneous single particle impedance and fluorescence analysis on a chip

High speed simultaneous single particle impedance and fluorescence analysis on a chip
High speed simultaneous single particle impedance and fluorescence analysis on a chip
In this paper, we describe the design and function of a microchip which is used to detect, analyse and count single micron-sized particles at high speed. The device uses multi-frequency electrical impedance together with single particle fluorescence spectroscopy. Impedance is measured using microelectrodes fabricated within a microfluidic channel. Optical measurements are made by focussing a beam of light into a detection volume of the order of the size of the particle. Particles flowing through the device are dynamically focussed into the detection volume using dielectrophoresis. The operating principle of the device is demonstrated by detecting and analysing fluorescent latex particles at high speed.
high-throughput analysis, microfluidics, single particle analysis, biochip
367-370
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Holmes, David
eeff86f7-ab4b-4795-9a01-82ce1275e34f
Green, Nicolas G
d9b47269-c426-41fd-a41d-5f4579faa581
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Holmes, David
eeff86f7-ab4b-4795-9a01-82ce1275e34f
Green, Nicolas G
d9b47269-c426-41fd-a41d-5f4579faa581

Morgan, Hywel, Holmes, David and Green, Nicolas G (2006) High speed simultaneous single particle impedance and fluorescence analysis on a chip. [in special issue: Second International Conference on Advanced Materials and Nanotechnology] Current Applied Physics, 6 (3), 367-370. (doi:10.1016/j.cap.2005.11.020).

Record type: Article

Abstract

In this paper, we describe the design and function of a microchip which is used to detect, analyse and count single micron-sized particles at high speed. The device uses multi-frequency electrical impedance together with single particle fluorescence spectroscopy. Impedance is measured using microelectrodes fabricated within a microfluidic channel. Optical measurements are made by focussing a beam of light into a detection volume of the order of the size of the particle. Particles flowing through the device are dynamically focussed into the detection volume using dielectrophoresis. The operating principle of the device is demonstrated by detecting and analysing fluorescent latex particles at high speed.

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More information

Published date: June 2006
Keywords: high-throughput analysis, microfluidics, single particle analysis, biochip
Organisations: Electronics & Computer Science

Identifiers

Local EPrints ID: 355528
URI: https://eprints.soton.ac.uk/id/eprint/355528
PURE UUID: 0cfb85a1-3044-440e-b6a5-f5b9ac2f70c3
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676
ORCID for Nicolas G Green: ORCID iD orcid.org/0000-0001-9230-4455

Catalogue record

Date deposited: 22 Oct 2013 11:31
Last modified: 07 Aug 2019 00:43

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