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Embossing of micropatterned ceramics and their cellular response

Embossing of micropatterned ceramics and their cellular response
Embossing of micropatterned ceramics and their cellular response
The aim of this work is to investigate the use of microtopographies in providing physical cues to modulate the cellular response of human mesenchymal stem cells on ceramics. Two microgrooved patterns (100 ?m/50 ?m, 10 ?m/10 ?m groove/pitch) were transcribed reversely onto alumina green ceramic tapes via an embossing technique followed by sintering. Characterization of the micropatterned alumina surfaces and their cellular response was carried out. Spread and polygonal cell morphologies were observed on the wider groove (50 ?m/100 ?m) surface. Cells seeded onto the narrow groove (10 ?m/10 ?m) surface aligned themselves alongside the grooves, resulting in more elongated cell morphology. More osteoid matrix nodules shown by osteopontin and osteocalcin biomarkers were detected on the larger grooved surfaces after cell culture of 21 days, indicating a greater level of osteogenicity. This study has shown that micropatterned wider groove (50 ?m) topographies are more suitable surfaces for improving osseointegration of ceramic implants.
ceramics, embossing, micropatterns, osseointegration, stem cells
1549-3296
3247-3255
Nadeem, Danish
60e0b460-732f-452c-9128-7399e4c1f90c
Sjostrom, Terje
c1615dd5-a794-4b7a-ad5e-eff8df229b59
Wilkinson, Andrew
f3c55917-62cb-4902-aad4-07925dc69ea9
Smith, Carol-Anne
396c04b3-8e95-463a-abf4-cd1e0e98611a
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Dalby, Matt J.
14879553-4dca-4cb7-a9d8-0d92ea13e5e1
Su, Bo
37e0f5b2-72ab-48e5-87c0-cabdfd83cc21
Nadeem, Danish
60e0b460-732f-452c-9128-7399e4c1f90c
Sjostrom, Terje
c1615dd5-a794-4b7a-ad5e-eff8df229b59
Wilkinson, Andrew
f3c55917-62cb-4902-aad4-07925dc69ea9
Smith, Carol-Anne
396c04b3-8e95-463a-abf4-cd1e0e98611a
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Dalby, Matt J.
14879553-4dca-4cb7-a9d8-0d92ea13e5e1
Su, Bo
37e0f5b2-72ab-48e5-87c0-cabdfd83cc21

Nadeem, Danish, Sjostrom, Terje, Wilkinson, Andrew, Smith, Carol-Anne, Oreffo, Richard O.C., Dalby, Matt J. and Su, Bo (2013) Embossing of micropatterned ceramics and their cellular response. Journal of Biomedical Materials Research Part A, 101 (11), 3247-3255. (doi:10.1002/jbm.a.34622).

Record type: Article

Abstract

The aim of this work is to investigate the use of microtopographies in providing physical cues to modulate the cellular response of human mesenchymal stem cells on ceramics. Two microgrooved patterns (100 ?m/50 ?m, 10 ?m/10 ?m groove/pitch) were transcribed reversely onto alumina green ceramic tapes via an embossing technique followed by sintering. Characterization of the micropatterned alumina surfaces and their cellular response was carried out. Spread and polygonal cell morphologies were observed on the wider groove (50 ?m/100 ?m) surface. Cells seeded onto the narrow groove (10 ?m/10 ?m) surface aligned themselves alongside the grooves, resulting in more elongated cell morphology. More osteoid matrix nodules shown by osteopontin and osteocalcin biomarkers were detected on the larger grooved surfaces after cell culture of 21 days, indicating a greater level of osteogenicity. This study has shown that micropatterned wider groove (50 ?m) topographies are more suitable surfaces for improving osseointegration of ceramic implants.

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More information

Accepted/In Press date: 2013
e-pub ahead of print date: April 2013
Published date: 3 April 2013
Keywords: ceramics, embossing, micropatterns, osseointegration, stem cells
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 355838
URI: http://eprints.soton.ac.uk/id/eprint/355838
ISSN: 1549-3296
PURE UUID: 727a4844-dd4a-4bea-99df-263f054a3ef0
ORCID for Richard O.C. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

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Date deposited: 06 Sep 2013 09:30
Last modified: 28 Oct 2023 01:46

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Contributors

Author: Danish Nadeem
Author: Terje Sjostrom
Author: Andrew Wilkinson
Author: Carol-Anne Smith
Author: Matt J. Dalby
Author: Bo Su

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