The effect of porosity of a biphasic ceramic scaffold on human skeletal stem cell growth and differentiation in vivo.
The effect of porosity of a biphasic ceramic scaffold on human skeletal stem cell growth and differentiation in vivo.
Skeletal stem cell (SSC) growth on a novel porous HA/TCP scaffold has been investigated in vivo. The effect of porosity on osteogenic differentiation was assessed by comparing two groups of scaffolds with differing porosity but controlled pore size. Histology, microCT, scanning electron microscopy, and biochemical analysis were used to assess SSC proliferation and differentiation. The 45 pores per inch (ppi) scaffold demonstrated a greater increase in density than the 30 ppi scaffold following in vivo culture, and a reduction in dimensions of the pores and channels of the higher porosity scaffold was observed, indicating generation of new tissue within the pores. All scaffolds supported SSC proliferation but the higher scaffold porosity augmented osteogenic differentiation. ALP specific activity was enhanced on the 45 ppi scaffold compared to the 30 ppi scaffold. These studies demonstrate the importance of porosity in scaffold design and impact therein for tissue engineering application.
scaffold, porosity, skeletal stem cell, osteogenic differentiation, proliferation
3431-3437
Aarvold, Alexander
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Smith, James O.
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Tayton, Edward R.
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Lanham, Stuart A.
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Chaudhuri, Julian B.
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Turner, Irene G.
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Oreffo, Richard O.C.
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December 2013
Aarvold, Alexander
11dc317f-47fd-4b2c-b0a6-78688c679b5a
Smith, James O.
027f2a5a-1966-4077-97a7-f70d2e6b06b2
Tayton, Edward R.
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Lanham, Stuart A.
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Chaudhuri, Julian B.
40daebdf-c69f-486d-a4d8-a07f4789a40d
Turner, Irene G.
1f4c3f31-a3fe-4194-a63d-94ce221f410d
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Aarvold, Alexander, Smith, James O., Tayton, Edward R., Lanham, Stuart A., Chaudhuri, Julian B., Turner, Irene G. and Oreffo, Richard O.C.
(2013)
The effect of porosity of a biphasic ceramic scaffold on human skeletal stem cell growth and differentiation in vivo.
Journal of Biomedical Materials Research Part A, 101 (12), .
(doi:10.1002/jbm.a.34646).
(PMID:23568640)
Abstract
Skeletal stem cell (SSC) growth on a novel porous HA/TCP scaffold has been investigated in vivo. The effect of porosity on osteogenic differentiation was assessed by comparing two groups of scaffolds with differing porosity but controlled pore size. Histology, microCT, scanning electron microscopy, and biochemical analysis were used to assess SSC proliferation and differentiation. The 45 pores per inch (ppi) scaffold demonstrated a greater increase in density than the 30 ppi scaffold following in vivo culture, and a reduction in dimensions of the pores and channels of the higher porosity scaffold was observed, indicating generation of new tissue within the pores. All scaffolds supported SSC proliferation but the higher scaffold porosity augmented osteogenic differentiation. ALP specific activity was enhanced on the 45 ppi scaffold compared to the 30 ppi scaffold. These studies demonstrate the importance of porosity in scaffold design and impact therein for tissue engineering application.
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Accepted/In Press date: 25 January 2013
e-pub ahead of print date: 9 April 2013
Published date: December 2013
Keywords:
scaffold, porosity, skeletal stem cell, osteogenic differentiation, proliferation
Organisations:
Human Development & Health
Identifiers
Local EPrints ID: 355841
URI: http://eprints.soton.ac.uk/id/eprint/355841
ISSN: 1549-3296
PURE UUID: bcd0eafc-9642-4d99-beec-c960cf60e064
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Date deposited: 06 Sep 2013 11:01
Last modified: 15 Mar 2024 03:04
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Contributors
Author:
Alexander Aarvold
Author:
James O. Smith
Author:
Edward R. Tayton
Author:
Stuart A. Lanham
Author:
Julian B. Chaudhuri
Author:
Irene G. Turner
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