The University of Southampton
University of Southampton Institutional Repository

The effect of porosity of a biphasic ceramic scaffold on human skeletal stem cell growth and differentiation in vivo.

The effect of porosity of a biphasic ceramic scaffold on human skeletal stem cell growth and differentiation in vivo.
The effect of porosity of a biphasic ceramic scaffold on human skeletal stem cell growth and differentiation in vivo.
Skeletal stem cell (SSC) growth on a novel porous HA/TCP scaffold has been investigated in vivo. The effect of porosity on osteogenic differentiation was assessed by comparing two groups of scaffolds with differing porosity but controlled pore size. Histology, microCT, scanning electron microscopy, and biochemical analysis were used to assess SSC proliferation and differentiation. The 45 pores per inch (ppi) scaffold demonstrated a greater increase in density than the 30 ppi scaffold following in vivo culture, and a reduction in dimensions of the pores and channels of the higher porosity scaffold was observed, indicating generation of new tissue within the pores. All scaffolds supported SSC proliferation but the higher scaffold porosity augmented osteogenic differentiation. ALP specific activity was enhanced on the 45 ppi scaffold compared to the 30 ppi scaffold. These studies demonstrate the importance of porosity in scaffold design and impact therein for tissue engineering application.
scaffold, porosity, skeletal stem cell, osteogenic differentiation, proliferation
1549-3296
3431-3437
Aarvold, Alexander
11dc317f-47fd-4b2c-b0a6-78688c679b5a
Smith, James O.
027f2a5a-1966-4077-97a7-f70d2e6b06b2
Tayton, Edward R.
2c0e26d2-1f5f-498d-9ff7-d22200f19b3a
Lanham, Stuart A.
28fdbbef-e3b6-4fdf-bd0f-4968eeb614d6
Chaudhuri, Julian B.
40daebdf-c69f-486d-a4d8-a07f4789a40d
Turner, Irene G.
1f4c3f31-a3fe-4194-a63d-94ce221f410d
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Aarvold, Alexander
11dc317f-47fd-4b2c-b0a6-78688c679b5a
Smith, James O.
027f2a5a-1966-4077-97a7-f70d2e6b06b2
Tayton, Edward R.
2c0e26d2-1f5f-498d-9ff7-d22200f19b3a
Lanham, Stuart A.
28fdbbef-e3b6-4fdf-bd0f-4968eeb614d6
Chaudhuri, Julian B.
40daebdf-c69f-486d-a4d8-a07f4789a40d
Turner, Irene G.
1f4c3f31-a3fe-4194-a63d-94ce221f410d
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778

Aarvold, Alexander, Smith, James O., Tayton, Edward R., Lanham, Stuart A., Chaudhuri, Julian B., Turner, Irene G. and Oreffo, Richard O.C. (2013) The effect of porosity of a biphasic ceramic scaffold on human skeletal stem cell growth and differentiation in vivo. Journal of Biomedical Materials Research Part A, 101 (12), 3431-3437. (doi:10.1002/jbm.a.34646). (PMID:23568640)

Record type: Article

Abstract

Skeletal stem cell (SSC) growth on a novel porous HA/TCP scaffold has been investigated in vivo. The effect of porosity on osteogenic differentiation was assessed by comparing two groups of scaffolds with differing porosity but controlled pore size. Histology, microCT, scanning electron microscopy, and biochemical analysis were used to assess SSC proliferation and differentiation. The 45 pores per inch (ppi) scaffold demonstrated a greater increase in density than the 30 ppi scaffold following in vivo culture, and a reduction in dimensions of the pores and channels of the higher porosity scaffold was observed, indicating generation of new tissue within the pores. All scaffolds supported SSC proliferation but the higher scaffold porosity augmented osteogenic differentiation. ALP specific activity was enhanced on the 45 ppi scaffold compared to the 30 ppi scaffold. These studies demonstrate the importance of porosity in scaffold design and impact therein for tissue engineering application.

Text
The effect of porosity of a biphasic ceramic scaffold on human skeletal stem cell growth and differentiation in vivo.pdf - Version of Record
Restricted to Repository staff only
Request a copy

More information

Accepted/In Press date: 25 January 2013
e-pub ahead of print date: 9 April 2013
Published date: December 2013
Keywords: scaffold, porosity, skeletal stem cell, osteogenic differentiation, proliferation
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 355841
URI: https://eprints.soton.ac.uk/id/eprint/355841
ISSN: 1549-3296
PURE UUID: bcd0eafc-9642-4d99-beec-c960cf60e064
ORCID for Stuart A. Lanham: ORCID iD orcid.org/0000-0002-4516-264X
ORCID for Richard O.C. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 06 Sep 2013 11:01
Last modified: 20 Jul 2019 01:16

Export record

Altmetrics

Contributors

Author: Alexander Aarvold
Author: James O. Smith
Author: Edward R. Tayton
Author: Stuart A. Lanham ORCID iD
Author: Julian B. Chaudhuri
Author: Irene G. Turner

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×