Tryptophan insertion mutagenesis of liver fatty acid-binding proteinAcid-binding Protein


Hagan, R.M., Worner-Gibbs, J. and Wilton, D.C. (2004) Tryptophan insertion mutagenesis of liver fatty acid-binding proteinAcid-binding Protein The Journal of Biological Chemistry, 280, (3), pp. 1782-1789. (doi:10.1074/jbc.M407131200).

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Description/Abstract

Liver fatty acid-binding protein (FABP) binds a variety of non-polar anionic ligands including fatty acids, fatty acyl CoAs, and bile acids. Previously we prepared charge reversal mutants and demonstrated the importance of lysine residues within the portal region in ligand and membrane binding. We have now prepared several tryptophan-containing mutants within the portal region, and one tryptophan at position 28 (L28W) has proved remarkably effective as an intrinsic probe to further study ligand binding. The fluorescence of the L28W mutant was very sensitive to fatty acid and bile acid binding where a large (up to 4-fold) fluorescence enhancement was obtained. In contrast, the binding of oleoyl CoA reduced tryptophan fluorescence. Positive cooperativity for fatty acid binding was observed while detailed information on the orientation of binding of bile acid derivatives was obtained. The ability of bound oleoyl CoA to reduce the fluorescence of L28W provided an opportunity to demonstrate that fatty acyl CoAs can compete with fatty acids for binding to liver FABP under physiological conditions, further highlighting the role of fatty acyl CoAs in modulating FABP function in the cell.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1074/jbc.M407131200
ISSNs: 0021-9258 (print)
Related URLs:
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ePrint ID: 35585
Date :
Date Event
25 June 2004Submitted
12 November 2004Published
Date Deposited: 19 May 2006
Last Modified: 16 Apr 2017 22:09
Further Information:Google Scholar
URI: http://eprints.soton.ac.uk/id/eprint/35585

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