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Tryptophan insertion mutagenesis of liver fatty acid-binding protein Acid-binding Protein

Tryptophan insertion mutagenesis of liver fatty acid-binding protein Acid-binding Protein
Tryptophan insertion mutagenesis of liver fatty acid-binding protein Acid-binding Protein
Liver fatty acid-binding protein (FABP) binds a variety of non-polar anionic ligands including fatty acids, fatty acyl CoAs, and bile acids. Previously we prepared charge reversal mutants and demonstrated the importance of lysine residues within the portal region in ligand and membrane binding. We have now prepared several tryptophan-containing mutants within the portal region, and one tryptophan at position 28 (I.28W) has proved remarkably effective as an intrinsic probe to further study ligand binding. The fluorescence of the L28W mutant was very sensitive to fatty acid and bile acid binding where a large (up to 4-fold) fluorescence enhancement was obtained. In contrast, the binding of oleoyl CoA reduced tryptophan fluorescence. Positive cooperativity for fatty acid binding was observed while detailed information on the orientation of binding of bile acid derivatives was obtained. The ability of bound oleoyl CoA to reduce the fluorescence of L28W provided an opportunity to demonstrate that fatty acyl CoAs can compete with fatty acids for binding to liver FABP under physiological conditions, further highlighting the role of fatty acyl CoAs in modulating FABP function in the cell.
0021-9258
1782-1789
Hagan, R.M.
f835ad70-c6bd-4b6e-95a5-d047eff291b9
Worner-Gibbs, J.
b9353fab-f4de-4fbb-9750-1c2ca7ab1648
Wilton, D.C.
4b995f66-ad6c-4d96-9179-c64f3b54466a
Hagan, R.M.
f835ad70-c6bd-4b6e-95a5-d047eff291b9
Worner-Gibbs, J.
b9353fab-f4de-4fbb-9750-1c2ca7ab1648
Wilton, D.C.
4b995f66-ad6c-4d96-9179-c64f3b54466a

Hagan, R.M., Worner-Gibbs, J. and Wilton, D.C. (2004) Tryptophan insertion mutagenesis of liver fatty acid-binding protein Acid-binding Protein. The Journal of Biological Chemistry, 280 (3), 1782-1789. (doi:10.1074/jbc.M407131200).

Record type: Article

Abstract

Liver fatty acid-binding protein (FABP) binds a variety of non-polar anionic ligands including fatty acids, fatty acyl CoAs, and bile acids. Previously we prepared charge reversal mutants and demonstrated the importance of lysine residues within the portal region in ligand and membrane binding. We have now prepared several tryptophan-containing mutants within the portal region, and one tryptophan at position 28 (I.28W) has proved remarkably effective as an intrinsic probe to further study ligand binding. The fluorescence of the L28W mutant was very sensitive to fatty acid and bile acid binding where a large (up to 4-fold) fluorescence enhancement was obtained. In contrast, the binding of oleoyl CoA reduced tryptophan fluorescence. Positive cooperativity for fatty acid binding was observed while detailed information on the orientation of binding of bile acid derivatives was obtained. The ability of bound oleoyl CoA to reduce the fluorescence of L28W provided an opportunity to demonstrate that fatty acyl CoAs can compete with fatty acids for binding to liver FABP under physiological conditions, further highlighting the role of fatty acyl CoAs in modulating FABP function in the cell.

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Submitted date: 25 June 2004
Published date: 12 November 2004

Identifiers

Local EPrints ID: 35585
URI: http://eprints.soton.ac.uk/id/eprint/35585
ISSN: 0021-9258
PURE UUID: 5848e902-5d4e-4855-933e-eb18265a4c41

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Date deposited: 19 May 2006
Last modified: 08 Jan 2022 01:06

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Contributors

Author: R.M. Hagan
Author: J. Worner-Gibbs
Author: D.C. Wilton

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