Hydroxyapatite coated with insulin-like growth factor-1 (IGF-1) stimulates human osteoblast activity in vitro
Hydroxyapatite coated with insulin-like growth factor-1 (IGF-1) stimulates human osteoblast activity in vitro
We studied the effects of a hydroxyapatite-tricalcium phosphate material coated with Insulin-like Growth Factor 1 (IGF1) on cell growth, collagen synthesis and alkaline phosphatase activity (ALP) of human osteoblasts in vitro. Cell proliferation was stimulated when osteoblasts were incubated with untreated hydroxyapatite (HA) and it was further increased by exposure to IGF1-coated HA. 3H-Proline uptake was significantly increased by treatment with either HA or IGF1-coated HA but no significant differences were found between these two groups. ALP activity was enhanced by exposure to HA, with respect to the control, and further increased by treatment with IGF1-coated HA. Our findings suggest that HA is useful for promoting osteoblast activity and IGF1 may help to improve its biological characteristics.
217-220
Zambonin, Giovanni
7098daab-eaf4-4b35-ab93-ade0e974ad4c
Grano, Maria
03775b39-0a25-49ff-b390-3b0227eafa9c
Greco, Giovanni
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Oreffo, Richard O.C.
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Triffitt, James T.
0013314b-4d10-4351-b78b-fc0cfec362f8
Zambonin, Giovanni
7098daab-eaf4-4b35-ab93-ade0e974ad4c
Grano, Maria
03775b39-0a25-49ff-b390-3b0227eafa9c
Greco, Giovanni
7b59d858-a4c3-4ba5-aab8-da51527f3324
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Triffitt, James T.
0013314b-4d10-4351-b78b-fc0cfec362f8
Zambonin, Giovanni, Grano, Maria, Greco, Giovanni, Oreffo, Richard O.C. and Triffitt, James T.
(1999)
Hydroxyapatite coated with insulin-like growth factor-1 (IGF-1) stimulates human osteoblast activity in vitro.
Acta Orthopaedica Scandinavica, 70 (2), .
(PMID:10366928)
Abstract
We studied the effects of a hydroxyapatite-tricalcium phosphate material coated with Insulin-like Growth Factor 1 (IGF1) on cell growth, collagen synthesis and alkaline phosphatase activity (ALP) of human osteoblasts in vitro. Cell proliferation was stimulated when osteoblasts were incubated with untreated hydroxyapatite (HA) and it was further increased by exposure to IGF1-coated HA. 3H-Proline uptake was significantly increased by treatment with either HA or IGF1-coated HA but no significant differences were found between these two groups. ALP activity was enhanced by exposure to HA, with respect to the control, and further increased by treatment with IGF1-coated HA. Our findings suggest that HA is useful for promoting osteoblast activity and IGF1 may help to improve its biological characteristics.
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e-pub ahead of print date: 1 April 1999
Organisations:
Human Development & Health
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Local EPrints ID: 355861
URI: http://eprints.soton.ac.uk/id/eprint/355861
ISSN: 0001-6470
PURE UUID: 8b85ec53-982b-4800-918b-a7bd81826198
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Date deposited: 22 Aug 2013 15:17
Last modified: 09 Jan 2022 03:02
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Contributors
Author:
Giovanni Zambonin
Author:
Maria Grano
Author:
Giovanni Greco
Author:
James T. Triffitt
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