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A droplet-based microfluidic platform for high-throughput, multi-parameter screening of photosensitizer activity

A droplet-based microfluidic platform for high-throughput, multi-parameter screening of photosensitizer activity
A droplet-based microfluidic platform for high-throughput, multi-parameter screening of photosensitizer activity
We present a fully integrated droplet-based microfluidic platform for the high-throughput assessment of photodynamic therapy photosensitizer (PDT) efficacy on Escherichia coli. The described platform is able to controllably encapsulate cells and photosensitizer within pL-volume droplets, incubate the droplets over the course of several days, add predetermined concentrations of viability assay agents, expose droplets to varying doses of electromagnetic radiation, and detect both live and dead cells online to score cell viability. The viability of cells after encapsulation and incubation is assessed in a direct fashion, and the viability scoring method is compared to model live/dead systems for calibration. Final results are validated against conventional colony forming unit assays. In addition, we show that the platform can be used to perform concurrent measurements of light and dark toxicity of the PDT agents and that the platform allows simultaneous measurement of experimental parameters that include dark toxicity, photosensitizer concentration, light dose, and oxygenation levels for the development and testing of PDT agents
0003-2700
130812161648003
Cho, Soongwon
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Kang, Dong-Ku
743db45a-ce9b-4084-b253-a6f8ba43e8c3
Sim, Steven
dff658a2-d9ac-4429-932e-162612de4a00
Geier, Florian
4e288a6b-2262-40d1-b021-c89e5f920924
Kim, Jin-Young
e94408b5-59dc-45a1-8909-694ebc480f19
Niu, Xize
f3d964fb-23b4-45db-92fe-02426e4e76fa
Edel, Joshua B.
8397afdd-a0dc-489b-83e8-58a75ca46732
Chang, Soo-Ik
8d023586-1286-4420-9e6a-d089d1c462f2
Wootton, Robert C.R.
69031cb6-7f29-430e-b6bd-722451d6f2a1
Elvira, Katherine S.
a9eada3b-a5cc-4d4b-b741-ba54d882db21
de Mello, Andrew J.
cba23f1f-bc9b-494b-953d-e9514bb52f68
Cho, Soongwon
0afaae85-b6f1-463f-bbcb-a81361c26de5
Kang, Dong-Ku
743db45a-ce9b-4084-b253-a6f8ba43e8c3
Sim, Steven
dff658a2-d9ac-4429-932e-162612de4a00
Geier, Florian
4e288a6b-2262-40d1-b021-c89e5f920924
Kim, Jin-Young
e94408b5-59dc-45a1-8909-694ebc480f19
Niu, Xize
f3d964fb-23b4-45db-92fe-02426e4e76fa
Edel, Joshua B.
8397afdd-a0dc-489b-83e8-58a75ca46732
Chang, Soo-Ik
8d023586-1286-4420-9e6a-d089d1c462f2
Wootton, Robert C.R.
69031cb6-7f29-430e-b6bd-722451d6f2a1
Elvira, Katherine S.
a9eada3b-a5cc-4d4b-b741-ba54d882db21
de Mello, Andrew J.
cba23f1f-bc9b-494b-953d-e9514bb52f68

Cho, Soongwon, Kang, Dong-Ku, Sim, Steven, Geier, Florian, Kim, Jin-Young, Niu, Xize, Edel, Joshua B., Chang, Soo-Ik, Wootton, Robert C.R., Elvira, Katherine S. and de Mello, Andrew J. (2013) A droplet-based microfluidic platform for high-throughput, multi-parameter screening of photosensitizer activity. Analytical Chemistry, 130812161648003. (doi:10.1021/ac4022067).

Record type: Article

Abstract

We present a fully integrated droplet-based microfluidic platform for the high-throughput assessment of photodynamic therapy photosensitizer (PDT) efficacy on Escherichia coli. The described platform is able to controllably encapsulate cells and photosensitizer within pL-volume droplets, incubate the droplets over the course of several days, add predetermined concentrations of viability assay agents, expose droplets to varying doses of electromagnetic radiation, and detect both live and dead cells online to score cell viability. The viability of cells after encapsulation and incubation is assessed in a direct fashion, and the viability scoring method is compared to model live/dead systems for calibration. Final results are validated against conventional colony forming unit assays. In addition, we show that the platform can be used to perform concurrent measurements of light and dark toxicity of the PDT agents and that the platform allows simultaneous measurement of experimental parameters that include dark toxicity, photosensitizer concentration, light dose, and oxygenation levels for the development and testing of PDT agents

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Published date: 2013
Organisations: Mechatronics

Identifiers

Local EPrints ID: 356043
URI: https://eprints.soton.ac.uk/id/eprint/356043
ISSN: 0003-2700
PURE UUID: ec5aa350-8f8a-4776-ac00-e858905de637

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Date deposited: 06 Sep 2013 07:33
Last modified: 18 Jul 2017 03:41

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Contributors

Author: Soongwon Cho
Author: Dong-Ku Kang
Author: Steven Sim
Author: Florian Geier
Author: Jin-Young Kim
Author: Xize Niu
Author: Joshua B. Edel
Author: Soo-Ik Chang
Author: Robert C.R. Wootton
Author: Katherine S. Elvira
Author: Andrew J. de Mello

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