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Measurements of the dielectric properties of peripheral blood mononuclear cells and trophoblast cells using AC electrokinetic techniques

Measurements of the dielectric properties of peripheral blood mononuclear cells and trophoblast cells using AC electrokinetic techniques
Measurements of the dielectric properties of peripheral blood mononuclear cells and trophoblast cells using AC electrokinetic techniques
The separation of trophoblast cells from the maternal circulation could provide a valuable diagnostic tool for prenatal diagnosis of genetic abnormalities. This has been attempted using antibody methods, but due to non-specificity of the antibodies, maternal cell contamination remains a problem. We have investigated the potential of dielectrophoretic separation methods as a means of isolating trophoblast cells from mixed peripheral blood mononuclear cells. To determine the potential of this method the dielectric properties of trophoblast cells and mixed peripheral blood mononuclear cells were measured using dielectrophoretic crossover and single cell electrorotation methods. Both dielectrophoretic crossover data and electrorotation data gave an average specific membrane capacitance of the peripheral blood mononuclear cells of 11.5 mF m(-2). Trophoblast cells prepared using three different methods had a higher average specific membrane capacitance in the range 13-18 mF m(-2). The differences in capacitance between the cell types could be exploited as the basis of an AC electrokinetic-based system for the separation of trophoblast cells from peripheral blood mononuclear cells.
0925-4439
313-322
Chan, K.
2fad0805-393b-4c6c-90a2-fd6fd9dfa29c
Morgan, H.
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Morgan, E.
788032a6-1607-4f2e-95a6-74260a6bcddb
Cameron, I.
7db95cd0-3b1f-43a6-a809-3ec53cbe304c
Thomas, M.
f5239f6a-49f5-4645-9f1c-37ae8fbcd0d5
Chan, K.
2fad0805-393b-4c6c-90a2-fd6fd9dfa29c
Morgan, H.
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Morgan, E.
788032a6-1607-4f2e-95a6-74260a6bcddb
Cameron, I.
7db95cd0-3b1f-43a6-a809-3ec53cbe304c
Thomas, M.
f5239f6a-49f5-4645-9f1c-37ae8fbcd0d5

Chan, K., Morgan, H., Morgan, E., Cameron, I. and Thomas, M. (2000) Measurements of the dielectric properties of peripheral blood mononuclear cells and trophoblast cells using AC electrokinetic techniques. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1500 (3), 313-322. (doi:10.1016/S0925-4439(99)00115-5). (PMID:10699373)

Record type: Article

Abstract

The separation of trophoblast cells from the maternal circulation could provide a valuable diagnostic tool for prenatal diagnosis of genetic abnormalities. This has been attempted using antibody methods, but due to non-specificity of the antibodies, maternal cell contamination remains a problem. We have investigated the potential of dielectrophoretic separation methods as a means of isolating trophoblast cells from mixed peripheral blood mononuclear cells. To determine the potential of this method the dielectric properties of trophoblast cells and mixed peripheral blood mononuclear cells were measured using dielectrophoretic crossover and single cell electrorotation methods. Both dielectrophoretic crossover data and electrorotation data gave an average specific membrane capacitance of the peripheral blood mononuclear cells of 11.5 mF m(-2). Trophoblast cells prepared using three different methods had a higher average specific membrane capacitance in the range 13-18 mF m(-2). The differences in capacitance between the cell types could be exploited as the basis of an AC electrokinetic-based system for the separation of trophoblast cells from peripheral blood mononuclear cells.

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More information

Published date: 17 March 2000
Organisations: Electronics & Computer Science

Identifiers

Local EPrints ID: 356171
URI: https://eprints.soton.ac.uk/id/eprint/356171
ISSN: 0925-4439
PURE UUID: 7fc4678e-05e4-42c7-a00f-10325e0e9f95
ORCID for H. Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 22 Nov 2013 14:05
Last modified: 06 Jun 2018 12:45

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