A his-tagged Melanocarpus albomycesLaccase and its electrochemistry upon immobilisation on NTA-modified electrodes and in conducting polymer films
A his-tagged Melanocarpus albomycesLaccase and its electrochemistry upon immobilisation on NTA-modified electrodes and in conducting polymer films
The article describes the construction, immobilisation and electrochemistry of histidine tagged laccase from Melanocarpus albomyces. A facile method of functionalisation of glassy carbon electrodes with nitrilotriacetic acid (NTA) using diazonium grafting and solid state chemistry is described. NTA-modified electrodes are shown to bind laccase which reduces oxygen at neutral pH in the presence of soluble redox mediator. Laccase-modified electrodes are also prepared by enzyme immobilisation within poly(aniline)/poly(vinylsulfonate) films. The polymer is found to efficiently retain the enzyme as well as provide direct electrical contact between the electrode and the enzyme active centre. Cyclic voltammetry reveals the direct electron transfer to the enzyme is dependent on the redox state of the polymer film
2225-2231
Sosna, Maciej
b089fe7e-6f5b-4e30-a43c-0cde0aa1fac4
Boer, Harry
0cc8dada-812a-4a0f-9f32-8f54eca8f503
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
2013
Sosna, Maciej
b089fe7e-6f5b-4e30-a43c-0cde0aa1fac4
Boer, Harry
0cc8dada-812a-4a0f-9f32-8f54eca8f503
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
Sosna, Maciej, Boer, Harry and Bartlett, Philip N.
(2013)
A his-tagged Melanocarpus albomycesLaccase and its electrochemistry upon immobilisation on NTA-modified electrodes and in conducting polymer films.
[in special issue: Photo- and Bioelectrochemistry]
ChemPhysChem, 14 (10), .
(doi:10.1002/cphc.201300340).
Abstract
The article describes the construction, immobilisation and electrochemistry of histidine tagged laccase from Melanocarpus albomyces. A facile method of functionalisation of glassy carbon electrodes with nitrilotriacetic acid (NTA) using diazonium grafting and solid state chemistry is described. NTA-modified electrodes are shown to bind laccase which reduces oxygen at neutral pH in the presence of soluble redox mediator. Laccase-modified electrodes are also prepared by enzyme immobilisation within poly(aniline)/poly(vinylsulfonate) films. The polymer is found to efficiently retain the enzyme as well as provide direct electrical contact between the electrode and the enzyme active centre. Cyclic voltammetry reveals the direct electron transfer to the enzyme is dependent on the redox state of the polymer film
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Published date: 2013
Organisations:
Electrochemistry
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Local EPrints ID: 356255
URI: http://eprints.soton.ac.uk/id/eprint/356255
ISSN: 1439-4235
PURE UUID: e6bcd85f-f9e9-420e-9c1e-b71cab092a03
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Date deposited: 30 Aug 2013 13:08
Last modified: 15 Mar 2024 02:44
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Author:
Maciej Sosna
Author:
Harry Boer
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