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Crystallization and preliminary X-ray diffraction analysis of calexcitin from Loligo pealei: a neuronal protein implicated in learning and memory

Crystallization and preliminary X-ray diffraction analysis of calexcitin from Loligo pealei: a neuronal protein implicated in learning and memory
Crystallization and preliminary X-ray diffraction analysis of calexcitin from Loligo pealei: a neuronal protein implicated in learning and memory
The neuronal protein calexcitin from the long-finned squid Loligo pealei has been expressed in Escherichia coli and purified to homogeneity. Calexcitin is a 22 kDa calcium-binding protein that becomes up-regulated in invertebrates following Pavlovian conditioning and is likely to be involved in signal transduction events associated with learning and memory. Recombinant squid calexcitin has been crystallized using the hanging-drop vapour-diffusion technique in the orthorhombic space group P212121. The unit-cell parameters of a = 46.6, b = 69.2, c = 134.8 Å suggest that the crystals contain two monomers per asymmetric unit and have a solvent content of 49%. This crystal form diffracts X-rays to at least 1.8 Å resolution and yields data of high quality using synchrotron radiation.
calexcitin, calcium-binding proteins
1744-3091
879-881
Beaven, G.D.E.
0316b282-3ada-4e5f-bafa-e30006657ee2
Erskine, P.T.
c77b60c5-b80c-4e6a-a103-bf57ecfcbcf6
Wright, J.N.
e53ee4b9-10f5-4365-a9f2-5a7b0eacd86d
Mohammed, F.
e6dd5a98-bea5-484a-873a-da499c034594
Gill, R.
95656ecb-604f-425e-ac66-7dd36e47d94d
Wood, S.P.
430faabf-7f5c-4cf6-9bcc-5955f5e09566
Vernon, J.
203d96d6-68ba-4318-b1ae-f1df35c722e0
Giese, K.P.
697b34d4-ef9a-4d99-89c8-5db903a45078
Cooper, J.B.
d9f0f6a8-1260-48fc-aa5c-3dbc650e3ec0
Beaven, G.D.E.
0316b282-3ada-4e5f-bafa-e30006657ee2
Erskine, P.T.
c77b60c5-b80c-4e6a-a103-bf57ecfcbcf6
Wright, J.N.
e53ee4b9-10f5-4365-a9f2-5a7b0eacd86d
Mohammed, F.
e6dd5a98-bea5-484a-873a-da499c034594
Gill, R.
95656ecb-604f-425e-ac66-7dd36e47d94d
Wood, S.P.
430faabf-7f5c-4cf6-9bcc-5955f5e09566
Vernon, J.
203d96d6-68ba-4318-b1ae-f1df35c722e0
Giese, K.P.
697b34d4-ef9a-4d99-89c8-5db903a45078
Cooper, J.B.
d9f0f6a8-1260-48fc-aa5c-3dbc650e3ec0

Beaven, G.D.E., Erskine, P.T., Wright, J.N., Mohammed, F., Gill, R., Wood, S.P., Vernon, J., Giese, K.P. and Cooper, J.B. (2005) Crystallization and preliminary X-ray diffraction analysis of calexcitin from Loligo pealei: a neuronal protein implicated in learning and memory. Acta Crystallographica Section F: Structural Biology Communications, 61 (10), 879-881. (doi:10.1107/S1744309105026758).

Record type: Article

Abstract

The neuronal protein calexcitin from the long-finned squid Loligo pealei has been expressed in Escherichia coli and purified to homogeneity. Calexcitin is a 22 kDa calcium-binding protein that becomes up-regulated in invertebrates following Pavlovian conditioning and is likely to be involved in signal transduction events associated with learning and memory. Recombinant squid calexcitin has been crystallized using the hanging-drop vapour-diffusion technique in the orthorhombic space group P212121. The unit-cell parameters of a = 46.6, b = 69.2, c = 134.8 Å suggest that the crystals contain two monomers per asymmetric unit and have a solvent content of 49%. This crystal form diffracts X-rays to at least 1.8 Å resolution and yields data of high quality using synchrotron radiation.

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More information

Submitted date: 13 September 2005
Published date: 1 October 2005
Keywords: calexcitin, calcium-binding proteins
Organisations: Biological Sciences

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Local EPrints ID: 35627
URI: https://eprints.soton.ac.uk/id/eprint/35627
ISSN: 1744-3091
PURE UUID: 982f4080-d483-4b9c-a7d7-82b54cb897c1

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Date deposited: 22 May 2006
Last modified: 17 Jul 2017 15:47

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