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A simple QM/MM approach for capturing polarization effects in protein-ligand binding free energy calculations

A simple QM/MM approach for capturing polarization effects in protein-ligand binding free energy calculations
A simple QM/MM approach for capturing polarization effects in protein-ligand binding free energy calculations
We present a molecular simulation protocol to compute free energies of binding, which combines a QM/MM correction term with rigorous classical free energy techniques, thereby accounting for electronic polarization effects. Relative free energies of binding are first computed using classical force fields, Monte Carlo sampling, and replica exchange thermodynamic integration. Snapshots of the configurations at the end points of the perturbation are then subjected to DFT-QM/MM single-point calculations using the B3LYP functional and a range of basis sets. The resulting quantum mechanical energies are then processed using the Zwanzig equation to give free energies incorporating electronic polarization. Our approach is conceptually simple and does not require tightly coupled QM and MM software. The method has been validated by calculating the relative free energies of hydration of methane and water and the relative free energy of binding of two inhibitors of cyclooxygenase-2. Closed thermodynamic cycles are obtained across different pathways, demonstrating the correctness of the technique, although significantly more sampling is required for the protein-ligand system. Our method offers a simple and effective way to incorporate quantum mechanical effects into computed free energies of binding.
1520-6106
4911-4926
Beierlein, Frank R.
2bf9edbd-ed57-42a5-a1b4-8a4cdea75692
Michel, Julien
3dfda20a-a6fa-4214-8c7d-578f550b9ad7
Essex, Jonathan W.
1f409cfe-6ba4-42e2-a0ab-a931826314b5
Beierlein, Frank R.
2bf9edbd-ed57-42a5-a1b4-8a4cdea75692
Michel, Julien
3dfda20a-a6fa-4214-8c7d-578f550b9ad7
Essex, Jonathan W.
1f409cfe-6ba4-42e2-a0ab-a931826314b5

Beierlein, Frank R., Michel, Julien and Essex, Jonathan W. (2011) A simple QM/MM approach for capturing polarization effects in protein-ligand binding free energy calculations. The Journal of Physical Chemistry B, 115 (17), 4911-4926. (doi:10.1021/jp109054j).

Record type: Article

Abstract

We present a molecular simulation protocol to compute free energies of binding, which combines a QM/MM correction term with rigorous classical free energy techniques, thereby accounting for electronic polarization effects. Relative free energies of binding are first computed using classical force fields, Monte Carlo sampling, and replica exchange thermodynamic integration. Snapshots of the configurations at the end points of the perturbation are then subjected to DFT-QM/MM single-point calculations using the B3LYP functional and a range of basis sets. The resulting quantum mechanical energies are then processed using the Zwanzig equation to give free energies incorporating electronic polarization. Our approach is conceptually simple and does not require tightly coupled QM and MM software. The method has been validated by calculating the relative free energies of hydration of methane and water and the relative free energy of binding of two inhibitors of cyclooxygenase-2. Closed thermodynamic cycles are obtained across different pathways, demonstrating the correctness of the technique, although significantly more sampling is required for the protein-ligand system. Our method offers a simple and effective way to incorporate quantum mechanical effects into computed free energies of binding.

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More information

Published date: April 2011
Organisations: Chemistry, Faculty of Natural and Environmental Sciences, Computational Systems Chemistry

Identifiers

Local EPrints ID: 356462
URI: https://eprints.soton.ac.uk/id/eprint/356462
ISSN: 1520-6106
PURE UUID: 0d236336-1f43-4b21-92b6-45e74e8a76c8
ORCID for Jonathan W. Essex: ORCID iD orcid.org/0000-0003-2639-2746

Catalogue record

Date deposited: 30 Sep 2013 15:25
Last modified: 20 Dec 2018 01:36

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