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An in vivo ratio control mechanism for phospholipid homeostasis: evidence from lipidomic studies

An in vivo ratio control mechanism for phospholipid homeostasis: evidence from lipidomic studies
An in vivo ratio control mechanism for phospholipid homeostasis: evidence from lipidomic studies
While it is widely accepted that the lipid composition of eukaryotic membranes is under homeostatic control, the mechanisms through which cells sense lipid composition are still the subject of debate. It has been postulated that membrane curvature elastic energy is the membrane property that is regulated by cells, and that lipid composition is maintained by a ratio control function derived from the concentrations of type II and type 0 lipids, weighted appropriately. We assess this proposal by seeking a signature of ratio control in quantified lipid composition data obtained by electrospray ionization mass spectrometry from over 40 independent asynchronous cell populations. Our approach revealed the existence of a universal 'pivot' lipid, which marks the boundary between type 0 lipids and type II lipids, and which is invariant between different cell types or cells grown under different conditions. The presence of such a pivot species is a distinctive signature of the operation in vivo, in human cell lines, of a control function that is consistent with the hypothesis that membrane elastic energy is homeostatically controlled.
1742-5689
20120854
Dymond, M.K.
362ab9f1-bf0c-4c51-bbd7-13bd256ecd40
Hague, C.V.
cb265162-2ae5-4594-8678-c770a87c7a4f
Postle, A.D.
0fa17988-b4a0-4cdc-819a-9ae15c5dad66
Attard, G.S.
3219075d-2364-4f00-aeb9-1d90f8cd0d36
Dymond, M.K.
362ab9f1-bf0c-4c51-bbd7-13bd256ecd40
Hague, C.V.
cb265162-2ae5-4594-8678-c770a87c7a4f
Postle, A.D.
0fa17988-b4a0-4cdc-819a-9ae15c5dad66
Attard, G.S.
3219075d-2364-4f00-aeb9-1d90f8cd0d36

Dymond, M.K., Hague, C.V., Postle, A.D. and Attard, G.S. (2012) An in vivo ratio control mechanism for phospholipid homeostasis: evidence from lipidomic studies. Journal of the Royal Society Interface, 10 (80), 20120854. (doi:10.1098/rsif.2012.0854). (PMID:23256189)

Record type: Article

Abstract

While it is widely accepted that the lipid composition of eukaryotic membranes is under homeostatic control, the mechanisms through which cells sense lipid composition are still the subject of debate. It has been postulated that membrane curvature elastic energy is the membrane property that is regulated by cells, and that lipid composition is maintained by a ratio control function derived from the concentrations of type II and type 0 lipids, weighted appropriately. We assess this proposal by seeking a signature of ratio control in quantified lipid composition data obtained by electrospray ionization mass spectrometry from over 40 independent asynchronous cell populations. Our approach revealed the existence of a universal 'pivot' lipid, which marks the boundary between type 0 lipids and type II lipids, and which is invariant between different cell types or cells grown under different conditions. The presence of such a pivot species is a distinctive signature of the operation in vivo, in human cell lines, of a control function that is consistent with the hypothesis that membrane elastic energy is homeostatically controlled.

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More information

Published date: 6 March 2012
Organisations: Chemistry, Faculty of Natural and Environmental Sciences, Computational Systems Chemistry

Identifiers

Local EPrints ID: 356570
URI: http://eprints.soton.ac.uk/id/eprint/356570
ISSN: 1742-5689
PURE UUID: 7e97c477-03cc-4736-9d7c-dac0445a02bf
ORCID for A.D. Postle: ORCID iD orcid.org/0000-0001-7361-0756
ORCID for G.S. Attard: ORCID iD orcid.org/0000-0001-8304-0742

Catalogue record

Date deposited: 16 Sep 2013 13:18
Last modified: 26 Nov 2019 02:09

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