The University of Southampton
University of Southampton Institutional Repository

Serial FIB/SEM imaging for quantitative 3D assessment of the osteocyte lacuno-canalicular network

Serial FIB/SEM imaging for quantitative 3D assessment of the osteocyte lacuno-canalicular network
Serial FIB/SEM imaging for quantitative 3D assessment of the osteocyte lacuno-canalicular network
Up to now, a quantitative three-dimensional (3D) assessment of the lacuno-canalicular network (LCN) within bone has not been achieved in a comprehensive way and the LCN has mostly been investigated using two-dimensional imaging methods only. First attempts for the 3D assessment of the osteocytes and their cell processes have been reported using different imaging techniques. Nevertheless, various experimental limitations allowed for assessment of isolated or incompletely interconnected osteocytes only. On the other hand, serial focused ion beam/scanning electron microscopy (FIB/SEM) currently seems to be a promising imaging method for quantitative 3D assessment of the LCN. However, combined 3D visualization and quantification of the LCN using serial FIB/SEM imaging has not been reported so far. The aim of this study was to provide a proof of concept that serial FIB/SEM meets all requirements for quantitative 3D imaging of the LCN. To this end, we developed a new bone sample preparation protocol for serial FIB/SEM imaging providing a resolution on the order of 30 nm. This technique was successfully applied to the mid-diaphysis of a mouse femur. Moreover, we devised and applied novel measures for subsequent quantitative 3D morphometry of the LCN. Briefly, serial FIB/SEM was shown to be an appropriate technique to quantify the morphology of the LCN truly in 3D. This will allow investigating bone matrix changes on an ultrastructural level, which result from aging, disease, and treatment
bone, ultrastructure, osteocyte lacunae, canaliculi, lacuno-canalicular network, 3D imaging, serial FIB/SEM
8756-3282
304-311
Schneider, Philipp
a810f925-4808-44e4-8a4a-a51586f9d7ad
Meier, Matias
afbffe86-2d00-43c7-aa28-844c74153a59
Wepf, Roger
990f9d94-58da-41a5-8059-4d08a3ae0784
Müller, Ralph
f881853a-540f-48f1-bb6d-e0cf1894e036
Schneider, Philipp
a810f925-4808-44e4-8a4a-a51586f9d7ad
Meier, Matias
afbffe86-2d00-43c7-aa28-844c74153a59
Wepf, Roger
990f9d94-58da-41a5-8059-4d08a3ae0784
Müller, Ralph
f881853a-540f-48f1-bb6d-e0cf1894e036

Schneider, Philipp, Meier, Matias, Wepf, Roger and Müller, Ralph (2011) Serial FIB/SEM imaging for quantitative 3D assessment of the osteocyte lacuno-canalicular network. Bone, 49 (2), 304-311. (doi:10.1016/j.bone.2011.04.005). (PMID:21514408)

Record type: Article

Abstract

Up to now, a quantitative three-dimensional (3D) assessment of the lacuno-canalicular network (LCN) within bone has not been achieved in a comprehensive way and the LCN has mostly been investigated using two-dimensional imaging methods only. First attempts for the 3D assessment of the osteocytes and their cell processes have been reported using different imaging techniques. Nevertheless, various experimental limitations allowed for assessment of isolated or incompletely interconnected osteocytes only. On the other hand, serial focused ion beam/scanning electron microscopy (FIB/SEM) currently seems to be a promising imaging method for quantitative 3D assessment of the LCN. However, combined 3D visualization and quantification of the LCN using serial FIB/SEM imaging has not been reported so far. The aim of this study was to provide a proof of concept that serial FIB/SEM meets all requirements for quantitative 3D imaging of the LCN. To this end, we developed a new bone sample preparation protocol for serial FIB/SEM imaging providing a resolution on the order of 30 nm. This technique was successfully applied to the mid-diaphysis of a mouse femur. Moreover, we devised and applied novel measures for subsequent quantitative 3D morphometry of the LCN. Briefly, serial FIB/SEM was shown to be an appropriate technique to quantify the morphology of the LCN truly in 3D. This will allow investigating bone matrix changes on an ultrastructural level, which result from aging, disease, and treatment

Full text not available from this repository.

More information

Published date: August 2011
Keywords: bone, ultrastructure, osteocyte lacunae, canaliculi, lacuno-canalicular network, 3D imaging, serial FIB/SEM
Organisations: Bioengineering Group

Identifiers

Local EPrints ID: 356760
URI: http://eprints.soton.ac.uk/id/eprint/356760
ISSN: 8756-3282
PURE UUID: 313b94c1-ef55-4ddf-9cf9-abc63ac4fdeb
ORCID for Philipp Schneider: ORCID iD orcid.org/0000-0001-7499-3576

Catalogue record

Date deposited: 13 Sep 2013 08:11
Last modified: 17 Dec 2019 01:36

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×