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Complexity, temporal stability, and clinical correlates of airway bacterial community composition in primary ciliary dyskinesia

Complexity, temporal stability, and clinical correlates of airway bacterial community composition in primary ciliary dyskinesia
Complexity, temporal stability, and clinical correlates of airway bacterial community composition in primary ciliary dyskinesia
Primary ciliary dyskinesia (PCD) is a genetic disease characterised by abnormalities in ciliary function, leading to compromised airway clearance and chronic bacterial infection of the upper and lower airways. The composition of these infections, and the relationships between their characteristics and disease, are ill-defined. We describe the first systematic, culture-independent evaluation of lower airway bacteriology in PCD. Thirty-three airway samples (26 sputa, 7 BAL) were collected from 24 PCD patients aged 4 to 73 years. 16S rRNA quantitative PCR and pyrosequencing were used to determine load and community composition. Bacterial load, which ranged from 1.3x10(4) to 5.2x10(9) cfu/ml, was positively correlated with age (p=0.002) but not lung function. Analysis of ?7000 16S rRNA sequences per sample identified bacterial species belonging to 128 genera. Concurrently collected paired samples showed high bacterial community similarity. Mean relative abundance of dominant genera was 64.5% (SD ± 24.5), with these taxa including those reported through standard diagnostic microbiology (members of the genera Pseudomonas, Haemophilus, and Streptococcus) and those requiring specific ex vivo growth conditions (members of the genera Prevotella and Porphyromonas). Significant correlations observed included a positive relationship between P. aeruginosa relative abundance and age, and a negative relationship between P. aeruginosa relative abundance and lung function. The genus Ralstonia was also found to contribute substantially to the bacterial communities of a number of patients. Follow-up samples from a subset of patients revealed high levels of community temporal stability. The detailed microbiological characterisation presented here provides a basis for a reassessment of the clinical management of PCD airway infections.
0095-1137
4029-4035
Rogers, Geraint B.
bd90d82a-4150-4e01-a755-b81559586da2
Carroll, Mary P.
f7e407a5-f7fa-4efd-a7f1-8e6140b86f50
Zain, Nur Masirah M.
c47faea3-9651-4e20-bd17-82c5f9beec44
Bruce, Kenneth D.
09fa4b3f-2183-4e6e-933d-70a3c1b20b5d
Lock, Karen
a2ace0db-3544-43fb-9d70-e08acf6e8eda
Walker, Woolf
2ef1d306-38f6-4517-9c4c-8b858f135257
Jones, Graeme
f26c7d03-cd84-40c8-ae3a-286e861a5a29
Daniels, Thomas W.V.
d635a2fb-96a1-46ec-8cdf-8eb44a4bd0f5
Lucas, Jane S.
5cb3546c-87b2-4e59-af48-402076e25313
Rogers, Geraint B.
bd90d82a-4150-4e01-a755-b81559586da2
Carroll, Mary P.
f7e407a5-f7fa-4efd-a7f1-8e6140b86f50
Zain, Nur Masirah M.
c47faea3-9651-4e20-bd17-82c5f9beec44
Bruce, Kenneth D.
09fa4b3f-2183-4e6e-933d-70a3c1b20b5d
Lock, Karen
a2ace0db-3544-43fb-9d70-e08acf6e8eda
Walker, Woolf
2ef1d306-38f6-4517-9c4c-8b858f135257
Jones, Graeme
f26c7d03-cd84-40c8-ae3a-286e861a5a29
Daniels, Thomas W.V.
d635a2fb-96a1-46ec-8cdf-8eb44a4bd0f5
Lucas, Jane S.
5cb3546c-87b2-4e59-af48-402076e25313

Rogers, Geraint B., Carroll, Mary P., Zain, Nur Masirah M., Bruce, Kenneth D., Lock, Karen, Walker, Woolf, Jones, Graeme, Daniels, Thomas W.V. and Lucas, Jane S. (2013) Complexity, temporal stability, and clinical correlates of airway bacterial community composition in primary ciliary dyskinesia. Journal of Clinical Microbiology, 51 (12), 4029-4035. (doi:10.1128/JCM.02164-13). (PMID:24068019)

Record type: Article

Abstract

Primary ciliary dyskinesia (PCD) is a genetic disease characterised by abnormalities in ciliary function, leading to compromised airway clearance and chronic bacterial infection of the upper and lower airways. The composition of these infections, and the relationships between their characteristics and disease, are ill-defined. We describe the first systematic, culture-independent evaluation of lower airway bacteriology in PCD. Thirty-three airway samples (26 sputa, 7 BAL) were collected from 24 PCD patients aged 4 to 73 years. 16S rRNA quantitative PCR and pyrosequencing were used to determine load and community composition. Bacterial load, which ranged from 1.3x10(4) to 5.2x10(9) cfu/ml, was positively correlated with age (p=0.002) but not lung function. Analysis of ?7000 16S rRNA sequences per sample identified bacterial species belonging to 128 genera. Concurrently collected paired samples showed high bacterial community similarity. Mean relative abundance of dominant genera was 64.5% (SD ± 24.5), with these taxa including those reported through standard diagnostic microbiology (members of the genera Pseudomonas, Haemophilus, and Streptococcus) and those requiring specific ex vivo growth conditions (members of the genera Prevotella and Porphyromonas). Significant correlations observed included a positive relationship between P. aeruginosa relative abundance and age, and a negative relationship between P. aeruginosa relative abundance and lung function. The genus Ralstonia was also found to contribute substantially to the bacterial communities of a number of patients. Follow-up samples from a subset of patients revealed high levels of community temporal stability. The detailed microbiological characterisation presented here provides a basis for a reassessment of the clinical management of PCD airway infections.

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Published date: 25 September 2013
Organisations: Clinical & Experimental Sciences

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Local EPrints ID: 358032
URI: http://eprints.soton.ac.uk/id/eprint/358032
ISSN: 0095-1137
PURE UUID: 86e6cbc5-f6a5-4e85-81f6-5c6acd67ad1d

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Date deposited: 08 Oct 2013 13:43
Last modified: 16 Jul 2019 21:21

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Contributors

Author: Geraint B. Rogers
Author: Mary P. Carroll
Author: Nur Masirah M. Zain
Author: Kenneth D. Bruce
Author: Karen Lock
Author: Woolf Walker
Author: Graeme Jones
Author: Thomas W.V. Daniels
Author: Jane S. Lucas

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