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Multiple myeloma shows no intra-disease clustering of immunoglobulin heavy chain genes

Multiple myeloma shows no intra-disease clustering of immunoglobulin heavy chain genes
Multiple myeloma shows no intra-disease clustering of immunoglobulin heavy chain genes
Background: Characterization of the immunoglobulin gene repertoire has improved our understanding of the immunopathogenesis of lymphoid tumors. Early B-lymphocyte precursors of multiple myeloma are known to exist and might be susceptible to antigenic drive.

Design and Methods: To verify this hypothesis, we collected a database of 345 fully readable multiple myeloma immunoglobulin sequences. We characterized the immunoglobulin repertoire, analyzed the somatic hypermutation load, and investigated for stereotyped receptor clusters.

Results: Compared to the normal immunoglobulin repertoire, multiple myeloma displayed only modest differences involving only a few genes, showing that the myeloma immunoglobulin repertoire is the least skewed among mature B-cell tumors. Median somatic hypermutation load was 7.8%; median length of complementarity determining-region 3 was 15.5 amino acids. Clustering analysis showed the absence of myeloma specific clusters and no similarity with published chronic lymphocytic leukemia or lymphoma subsets.

Conclusions: Analysis of multiple myeloma immunoglobulin repertoire does not support a pathogenetic role for antigen selection in this tumor.
multiple myeloma, immunoglobulin, antigen selection, heavy chain gene
0390-6078
849-853
Ferrero, Simone
22a0ef9d-d391-4705-a4b1-4c6338dbdea0
Capello, Daniela
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Svaldi, Mirija
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Boi, Michela
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Gatti, Daniela
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Drandi, Daniela
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Rossi, Davide
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Barbiero, Sara
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Mantoan, Barbara
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Mantella, Elisabetta
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Zanni, Manuela
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Ghione, Paola
0e52e0e3-421c-4da1-9961-ba8ce733e03d
Larocca, Alessandra
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Passera, Roberto
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Bertoni, Francesco
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Gattei, Valter
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Forconi, Francesco
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Laurenti, Luca
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Del Poeta, Giovanni
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Marasca, Roberto
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Cortelazzo, Sergio
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Gaidano, Gianluca
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Palumbo, Antonio
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Boccadoro, Mario
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Ladetto, Marco
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Ferrero, Simone
22a0ef9d-d391-4705-a4b1-4c6338dbdea0
Capello, Daniela
f4d381f4-aaa6-47ae-bebf-53b0aed65055
Svaldi, Mirija
c83a4de7-7b0c-49b8-9340-09a388428ab9
Boi, Michela
41825b3a-fb59-4196-bb65-e7de0fd151be
Gatti, Daniela
40ba74ae-4abf-436b-9041-bc9ce1fcd3ae
Drandi, Daniela
819ad430-52ed-4409-8e81-26a3b12d3adf
Rossi, Davide
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Barbiero, Sara
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Mantoan, Barbara
e3755f00-ad5c-4928-8648-24f78eea1015
Mantella, Elisabetta
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Zanni, Manuela
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Ghione, Paola
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Larocca, Alessandra
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Passera, Roberto
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Bertoni, Francesco
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Gattei, Valter
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Forconi, Francesco
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Laurenti, Luca
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Del Poeta, Giovanni
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Marasca, Roberto
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Cortelazzo, Sergio
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Gaidano, Gianluca
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Palumbo, Antonio
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Boccadoro, Mario
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Ladetto, Marco
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Ferrero, Simone, Capello, Daniela, Svaldi, Mirija, Boi, Michela, Gatti, Daniela, Drandi, Daniela, Rossi, Davide, Barbiero, Sara, Mantoan, Barbara, Mantella, Elisabetta, Zanni, Manuela, Ghione, Paola, Larocca, Alessandra, Passera, Roberto, Bertoni, Francesco, Gattei, Valter, Forconi, Francesco, Laurenti, Luca, Del Poeta, Giovanni, Marasca, Roberto, Cortelazzo, Sergio, Gaidano, Gianluca, Palumbo, Antonio, Boccadoro, Mario and Ladetto, Marco (2012) Multiple myeloma shows no intra-disease clustering of immunoglobulin heavy chain genes. Haematologica, 97 (6), 849-853. (doi:10.3324/haematol.2011.052852). (PMID:22207685)

Record type: Article

Abstract

Background: Characterization of the immunoglobulin gene repertoire has improved our understanding of the immunopathogenesis of lymphoid tumors. Early B-lymphocyte precursors of multiple myeloma are known to exist and might be susceptible to antigenic drive.

Design and Methods: To verify this hypothesis, we collected a database of 345 fully readable multiple myeloma immunoglobulin sequences. We characterized the immunoglobulin repertoire, analyzed the somatic hypermutation load, and investigated for stereotyped receptor clusters.

Results: Compared to the normal immunoglobulin repertoire, multiple myeloma displayed only modest differences involving only a few genes, showing that the myeloma immunoglobulin repertoire is the least skewed among mature B-cell tumors. Median somatic hypermutation load was 7.8%; median length of complementarity determining-region 3 was 15.5 amino acids. Clustering analysis showed the absence of myeloma specific clusters and no similarity with published chronic lymphocytic leukemia or lymphoma subsets.

Conclusions: Analysis of multiple myeloma immunoglobulin repertoire does not support a pathogenetic role for antigen selection in this tumor.

Full text not available from this repository.

More information

e-pub ahead of print date: 29 December 2011
Published date: 1 June 2012
Keywords: multiple myeloma, immunoglobulin, antigen selection, heavy chain gene
Organisations: Cancer Sciences

Identifiers

Local EPrints ID: 358109
URI: https://eprints.soton.ac.uk/id/eprint/358109
ISSN: 0390-6078
PURE UUID: 3da684ae-21d6-49c0-8277-e2da2699ccd0

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Date deposited: 04 Oct 2013 10:31
Last modified: 16 Jul 2019 21:21

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Contributors

Author: Simone Ferrero
Author: Daniela Capello
Author: Mirija Svaldi
Author: Michela Boi
Author: Daniela Gatti
Author: Daniela Drandi
Author: Davide Rossi
Author: Sara Barbiero
Author: Barbara Mantoan
Author: Elisabetta Mantella
Author: Manuela Zanni
Author: Paola Ghione
Author: Alessandra Larocca
Author: Roberto Passera
Author: Francesco Bertoni
Author: Valter Gattei
Author: Francesco Forconi
Author: Luca Laurenti
Author: Giovanni Del Poeta
Author: Roberto Marasca
Author: Sergio Cortelazzo
Author: Gianluca Gaidano
Author: Antonio Palumbo
Author: Mario Boccadoro
Author: Marco Ladetto

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