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Antigen-loaded ER microsomes from APC induce potent immune responses against viral infection

Antigen-loaded ER microsomes from APC induce potent immune responses against viral infection
Antigen-loaded ER microsomes from APC induce potent immune responses against viral infection
Although matured DC are capable of inducing effective primary and secondary immune responses in vivo, it is difficult to control the maturation and antigen loading in vitro. In this study, we show that ER-enriched microsomal membranes (microsomes) isolated from DC contain more peptide-receptive MHC I and II molecules than, and a similar level of costimulatory molecules to, their parental DC. After loading with defined antigenic peptides, the microsomes deliver antigenic peptide–MHC complexes (pMHC) to both CD4 and CD8 T cells effectively in vivo. The peptide-loaded microsomes accumulate in peripheral lymphoid organs and induce stronger immune responses than peptide-pulsed DC. The microsomal vaccines protect against acute viral infection. Our data demonstrate that peptide–MHC complexes armed microsomes from DC can be an important alternative to DC-based vaccines for protection from viral infection
0014-2980
85-95
Sofra, Vassiliki
d5c4f84b-1029-49a0-9fcf-70f74b1d7fbc
Mansour, Salah
4aecba5a-8387-4f7b-b766-0a9c309ccb8b
Liu, Mengya
d72a2049-4ee1-430f-a5ce-67b70b244fc6
Gao, Bin
763f5d11-2809-4cea-a518-7f55037bceba
Primpidou, Elisavet
9bc7f5c1-a6c0-4af5-8961-ef0e400fba89
Wang, Ping
5f7a5780-5969-4486-ab0c-c527e48b3c34
Li, Suling
d94659c7-6acc-452f-89d6-b418442514fa
Sofra, Vassiliki
d5c4f84b-1029-49a0-9fcf-70f74b1d7fbc
Mansour, Salah
4aecba5a-8387-4f7b-b766-0a9c309ccb8b
Liu, Mengya
d72a2049-4ee1-430f-a5ce-67b70b244fc6
Gao, Bin
763f5d11-2809-4cea-a518-7f55037bceba
Primpidou, Elisavet
9bc7f5c1-a6c0-4af5-8961-ef0e400fba89
Wang, Ping
5f7a5780-5969-4486-ab0c-c527e48b3c34
Li, Suling
d94659c7-6acc-452f-89d6-b418442514fa

Sofra, Vassiliki, Mansour, Salah, Liu, Mengya, Gao, Bin, Primpidou, Elisavet, Wang, Ping and Li, Suling (2009) Antigen-loaded ER microsomes from APC induce potent immune responses against viral infection. European Journal of Immunology, 39 (1), 85-95. (doi:10.1002/eji.200838443).

Record type: Article

Abstract

Although matured DC are capable of inducing effective primary and secondary immune responses in vivo, it is difficult to control the maturation and antigen loading in vitro. In this study, we show that ER-enriched microsomal membranes (microsomes) isolated from DC contain more peptide-receptive MHC I and II molecules than, and a similar level of costimulatory molecules to, their parental DC. After loading with defined antigenic peptides, the microsomes deliver antigenic peptide–MHC complexes (pMHC) to both CD4 and CD8 T cells effectively in vivo. The peptide-loaded microsomes accumulate in peripheral lymphoid organs and induce stronger immune responses than peptide-pulsed DC. The microsomal vaccines protect against acute viral infection. Our data demonstrate that peptide–MHC complexes armed microsomes from DC can be an important alternative to DC-based vaccines for protection from viral infection

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More information

Published date: January 2009
Additional Information: Vassiliki Sofra and Salah Mansour are joint first authors
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 358314
URI: https://eprints.soton.ac.uk/id/eprint/358314
ISSN: 0014-2980
PURE UUID: 977839a6-ec07-47ac-a06b-6109ed9bc342
ORCID for Salah Mansour: ORCID iD orcid.org/0000-0002-5982-734X

Catalogue record

Date deposited: 03 Oct 2013 12:15
Last modified: 20 Jul 2019 00:49

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Contributors

Author: Vassiliki Sofra
Author: Salah Mansour ORCID iD
Author: Mengya Liu
Author: Bin Gao
Author: Elisavet Primpidou
Author: Ping Wang
Author: Suling Li

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