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Removal of interproximal dental biofilms by high-velocity water microdrops

Removal of interproximal dental biofilms by high-velocity water microdrops
Removal of interproximal dental biofilms by high-velocity water microdrops
The influence of the impact of a high-velocity water microdrop on the detachment of Streptococcus mutans UA159 biofilms from the interproximal (IP) space of teeth in a training typodont was studied experimentally and computationally. Twelve-day-old S. mutans biofilms in the IP space were exposed to a prototype AirFloss delivering 115 µL water at a maximum exit velocity of 60 m/sec in a 30-msec burst. Using confocal microscopy and image analysis, we obtained quantitative measurements of the percentage removal of biofilms from different locations in the IP space. The 3D geometry of the typodont and the IP spaces was obtained by micro-computed tomography (µ-CT) imaging. We performed computational fluid dynamics (CFD) simulations to calculate the wall shear stress (?w) distribution caused by the drops on the tooth surface. A qualitative agreement and a quantitative relationship between experiments and simulations were achieved. The wall shear stress (?w) generated by the prototype AirFloss and its spatial distribution on the teeth surface played a key role in dictating the efficacy of biofilm removal in the IP space.
oral hygiene, streptococcus mutans, micro-computed tomography, microscopy, interproximal cleaning, dental plaque
0022-0345
68-73
Rmaile, Amir
86affd59-4497-4b56-8247-f0b014c6edb2
Carugo, Dario
0a4be6cd-e309-4ed8-a620-20256ce01179
Capretto, Lorenzo
0f3586b5-1560-49c1-a76b-59e74ea600ef
Aspiras, Marcelo
c8862cf7-23b3-438b-a56f-6ba9d2346fba
De Jager, Marko
4514de87-263b-4acd-9704-f6ba891c4c01
Ward, Marilyn
c0c450d1-882e-470f-b0d8-05b2e32b7fb1
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f
Rmaile, Amir
86affd59-4497-4b56-8247-f0b014c6edb2
Carugo, Dario
0a4be6cd-e309-4ed8-a620-20256ce01179
Capretto, Lorenzo
0f3586b5-1560-49c1-a76b-59e74ea600ef
Aspiras, Marcelo
c8862cf7-23b3-438b-a56f-6ba9d2346fba
De Jager, Marko
4514de87-263b-4acd-9704-f6ba891c4c01
Ward, Marilyn
c0c450d1-882e-470f-b0d8-05b2e32b7fb1
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f

Rmaile, Amir, Carugo, Dario, Capretto, Lorenzo, Aspiras, Marcelo, De Jager, Marko, Ward, Marilyn and Stoodley, Paul (2014) Removal of interproximal dental biofilms by high-velocity water microdrops. Journal of Dental Research, 93 (1), 68-73. (doi:10.1177/0022034513510945). (PMID:24170371)

Record type: Article

Abstract

The influence of the impact of a high-velocity water microdrop on the detachment of Streptococcus mutans UA159 biofilms from the interproximal (IP) space of teeth in a training typodont was studied experimentally and computationally. Twelve-day-old S. mutans biofilms in the IP space were exposed to a prototype AirFloss delivering 115 µL water at a maximum exit velocity of 60 m/sec in a 30-msec burst. Using confocal microscopy and image analysis, we obtained quantitative measurements of the percentage removal of biofilms from different locations in the IP space. The 3D geometry of the typodont and the IP spaces was obtained by micro-computed tomography (µ-CT) imaging. We performed computational fluid dynamics (CFD) simulations to calculate the wall shear stress (?w) distribution caused by the drops on the tooth surface. A qualitative agreement and a quantitative relationship between experiments and simulations were achieved. The wall shear stress (?w) generated by the prototype AirFloss and its spatial distribution on the teeth surface played a key role in dictating the efficacy of biofilm removal in the IP space.

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More information

Published date: 1 January 2014
Keywords: oral hygiene, streptococcus mutans, micro-computed tomography, microscopy, interproximal cleaning, dental plaque
Organisations: Bioengineering Group, nCATS Group

Identifiers

Local EPrints ID: 358518
URI: http://eprints.soton.ac.uk/id/eprint/358518
ISSN: 0022-0345
PURE UUID: a8b37a43-7f71-4bcd-90d9-a524682e4d58
ORCID for Paul Stoodley: ORCID iD orcid.org/0000-0001-6069-273X

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Date deposited: 10 Oct 2013 13:57
Last modified: 17 Dec 2019 01:42

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Contributors

Author: Amir Rmaile
Author: Dario Carugo
Author: Lorenzo Capretto
Author: Marcelo Aspiras
Author: Marko De Jager
Author: Marilyn Ward
Author: Paul Stoodley ORCID iD

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