Preparation of peptide functionalized gold nanoparticles using one pot EDC/sulfo-NHS coupling
Preparation of peptide functionalized gold nanoparticles using one pot EDC/sulfo-NHS coupling
Although carbodiimides and succinimides are broadly employed for the formation of amide bonds (i.e. in aminoacid coupling), their use in the coupling of peptides to water-soluble carboxylic terminated colloidal gold nanoparticles remains challenging. In this paper, we present an optimization study for the successful coupling of the KPQPRPLS peptideto spherical and rod-like colloidal gold nanoparticles. We show that the concentration, reaction time and the chemical environment are all critical to achieve the formation of robust, peptide-coated colloidal nanoparticles. Agarose gel electrophoresis was used for the characterization of conjugates.
10119-10123
Bartczak, Dorota
4d706d11-ee17-4e30-9ba8-1d4b3d878f71
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
16 August 2011
Bartczak, Dorota
4d706d11-ee17-4e30-9ba8-1d4b3d878f71
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
Bartczak, Dorota and Kanaras, Antonios G.
(2011)
Preparation of peptide functionalized gold nanoparticles using one pot EDC/sulfo-NHS coupling.
Langmuir, 27 (16), .
(doi:10.1021/la2022177).
Abstract
Although carbodiimides and succinimides are broadly employed for the formation of amide bonds (i.e. in aminoacid coupling), their use in the coupling of peptides to water-soluble carboxylic terminated colloidal gold nanoparticles remains challenging. In this paper, we present an optimization study for the successful coupling of the KPQPRPLS peptideto spherical and rod-like colloidal gold nanoparticles. We show that the concentration, reaction time and the chemical environment are all critical to achieve the formation of robust, peptide-coated colloidal nanoparticles. Agarose gel electrophoresis was used for the characterization of conjugates.
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Published date: 16 August 2011
Organisations:
Quantum, Light & Matter Group
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Local EPrints ID: 358639
URI: http://eprints.soton.ac.uk/id/eprint/358639
ISSN: 0743-7463
PURE UUID: d6ea65d3-7851-4ef7-83c6-c971e93ce3f4
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Date deposited: 10 Oct 2013 13:55
Last modified: 15 Mar 2024 03:29
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Author:
Dorota Bartczak
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