Preparation of peptide functionalized gold nanoparticles using one pot EDC/sulfo-NHS coupling
Preparation of peptide functionalized gold nanoparticles using one pot EDC/sulfo-NHS coupling
Although carbodiimides and succinimides are broadly employed for the formation of amide bonds (i.e. in aminoacid coupling), their use in the coupling of peptides to water-soluble carboxylic terminated colloidal gold nanoparticles remains challenging. In this paper, we present an optimization study for the successful coupling of the KPQPRPLS peptideto spherical and rod-like colloidal gold nanoparticles. We show that the concentration, reaction time and the chemical environment are all critical to achieve the formation of robust, peptide-coated colloidal nanoparticles. Agarose gel electrophoresis was used for the characterization of conjugates.
10119-10123
Bartczak, Dorota
4d706d11-ee17-4e30-9ba8-1d4b3d878f71
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
16 August 2011
Bartczak, Dorota
4d706d11-ee17-4e30-9ba8-1d4b3d878f71
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
Bartczak, Dorota and Kanaras, Antonios G.
(2011)
Preparation of peptide functionalized gold nanoparticles using one pot EDC/sulfo-NHS coupling.
Langmuir, 27 (16), .
(doi:10.1021/la2022177).
Abstract
Although carbodiimides and succinimides are broadly employed for the formation of amide bonds (i.e. in aminoacid coupling), their use in the coupling of peptides to water-soluble carboxylic terminated colloidal gold nanoparticles remains challenging. In this paper, we present an optimization study for the successful coupling of the KPQPRPLS peptideto spherical and rod-like colloidal gold nanoparticles. We show that the concentration, reaction time and the chemical environment are all critical to achieve the formation of robust, peptide-coated colloidal nanoparticles. Agarose gel electrophoresis was used for the characterization of conjugates.
Text
Kanaras langmuir 2011.pdf
- Version of Record
Restricted to Repository staff only
Request a copy
More information
Published date: 16 August 2011
Organisations:
Quantum, Light & Matter Group
Identifiers
Local EPrints ID: 358639
URI: http://eprints.soton.ac.uk/id/eprint/358639
ISSN: 0743-7463
PURE UUID: d6ea65d3-7851-4ef7-83c6-c971e93ce3f4
Catalogue record
Date deposited: 10 Oct 2013 13:55
Last modified: 22 Aug 2025 01:58
Export record
Altmetrics
Contributors
Author:
Dorota Bartczak
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics
