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Copper-free click chemistry as an emerging tool for the programmed ligation of DNA-functionalised gold nanoparticles

Copper-free click chemistry as an emerging tool for the programmed ligation of DNA-functionalised gold nanoparticles
Copper-free click chemistry as an emerging tool for the programmed ligation of DNA-functionalised gold nanoparticles
We demonstrate a new method to program the ligation of single stranded DNA-modified gold nanoparticles
using copper–free click chemistry. Gold nanoparticles functionalized with a discrete number of 3’-azide or
5’-alkyne modified oligonucleotides, can be brought together via a splint strand and covalently ‘clicked’,
in a simple one-pot reaction. This new approach to the assembly of gold nanoparticles is inherently advantageous
in comparison to the traditional enzymatic ligation. The chemical ligation is specific and takes place at room
temperature by simply mixing the particles without the need for special enzymatic conditions. The yield of
‘clicked’ nanoparticles can be as high as 92%. The ease of the copper-free, ‘click-ligation’ method allows
for its universal applicability and opens up new avenues in programmed nanoparticle organization.
2040-3364
7209-7212
Heuer-Jungemman, Amelie
08992fab-14db-46d3-a564-4db283ff6da5
Kirkwood, Robert
363c79d6-bb3a-4a1c-b62a-2b1b1bdb8104
El-Sagheer, Afaf H.
05b8295a-64ad-4fdf-ad57-c34934a46c04
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
Heuer-Jungemman, Amelie
08992fab-14db-46d3-a564-4db283ff6da5
Kirkwood, Robert
363c79d6-bb3a-4a1c-b62a-2b1b1bdb8104
El-Sagheer, Afaf H.
05b8295a-64ad-4fdf-ad57-c34934a46c04
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7

Heuer-Jungemman, Amelie, Kirkwood, Robert, El-Sagheer, Afaf H., Brown, Tom and Kanaras, Antonios G. (2013) Copper-free click chemistry as an emerging tool for the programmed ligation of DNA-functionalised gold nanoparticles. Nanoscale, 5 (16), 7209-7212. (doi:10.1039/C3NR02362A).

Record type: Article

Abstract

We demonstrate a new method to program the ligation of single stranded DNA-modified gold nanoparticles
using copper–free click chemistry. Gold nanoparticles functionalized with a discrete number of 3’-azide or
5’-alkyne modified oligonucleotides, can be brought together via a splint strand and covalently ‘clicked’,
in a simple one-pot reaction. This new approach to the assembly of gold nanoparticles is inherently advantageous
in comparison to the traditional enzymatic ligation. The chemical ligation is specific and takes place at room
temperature by simply mixing the particles without the need for special enzymatic conditions. The yield of
‘clicked’ nanoparticles can be as high as 92%. The ease of the copper-free, ‘click-ligation’ method allows
for its universal applicability and opens up new avenues in programmed nanoparticle organization.

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Published date: August 2013
Organisations: Quantum, Light & Matter Group

Identifiers

Local EPrints ID: 358645
URI: https://eprints.soton.ac.uk/id/eprint/358645
ISSN: 2040-3364
PURE UUID: ea81e9bd-5c47-4b18-bfdf-c1c6f7a49a2c
ORCID for Antonios G. Kanaras: ORCID iD orcid.org/0000-0002-9847-6706

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Date deposited: 10 Oct 2013 14:38
Last modified: 20 Jul 2019 00:51

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