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The trade-off between the light-harvesting and photoprotective functions of fucoxanthin-chlorophyll proteins dominates light acclimation in Emiliania huxleyi (clone CCMP 1516)

The trade-off between the light-harvesting and photoprotective functions of fucoxanthin-chlorophyll proteins dominates light acclimation in Emiliania huxleyi (clone CCMP 1516)
The trade-off between the light-harvesting and photoprotective functions of fucoxanthin-chlorophyll proteins dominates light acclimation in Emiliania huxleyi (clone CCMP 1516)
Mechanistic understanding of the costs and benefits of photoacclimation requires knowledge of how photophysiology is affected by changes in the molecular structure of the chloroplast.

We tested the hypothesis that changes in the light dependencies of photosynthesis, nonphotochemical quenching and PSII photoinactivation arises from changes in the abundances of chloroplast proteins in Emiliania huxleyi strain CCMP 1516 grown at 30 (Low Light; LL) and 1000 (High Light; HL) µmol photons m-2 s-1 photon flux densities.

Carbon-specific light-saturated gross photosynthesis rates were not significantly different between cells acclimated to LL and HL. Acclimation to LL benefited cells by increasing biomass-specific light absorption and gross photosynthesis rates under low light, whereas acclimation to HL benefited cells by reducing the rate of photoinactivation of PSII under high light. Differences in the relative abundances of proteins assigned to light-harvesting (Lhcf), photoprotection (LI818-like), and the photosystem II (PSII) core complex accompanied differences in photophysiology: specifically, Lhcf:PSII was greater under LL, whereas LI818:PSII was greater in HL.

Thus, photoacclimation in E. huxleyi involved a trade-off amongst the characteristics of light absorption and photoprotection, which could be attributed to changes in the abundance and composition of proteins in the light-harvesting antenna of PSII.
Emiliania huxleyi, gross photosynthesis, light acclimation, light harvesting, nonphotochemical quenching, photoinactivation, photoinhibition
0028-646X
74-85
McKew, Boyd A.
7c838b85-e911-4e34-bb2c-b09c53f33b2f
Davey, Phillip
8b92a766-9f90-450e-9bfc-0719bd854188
Finch, Stewart J.
825a4a70-fd54-47d7-84ca-a6280c34ed8e
Hopkins, Jason
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Lefebvre, Stephane C.
ceed4c2e-0543-4ed7-9827-7919853f8b2f
Metodiev, Metodi V.
ce078e77-0492-400a-b8ff-9c04f522bf8e
Oxborough, Kevin
16d1da54-ed48-400e-9acf-d03536a1401e
Raines, Christine A.
8d94a3f5-e2c5-40be-9741-a76c7144ec91
Lawson, Tracy
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Geider, Richard J.
f1432d5c-8c1d-48ab-ac52-e81ee5ce7f42
McKew, Boyd A.
7c838b85-e911-4e34-bb2c-b09c53f33b2f
Davey, Phillip
8b92a766-9f90-450e-9bfc-0719bd854188
Finch, Stewart J.
825a4a70-fd54-47d7-84ca-a6280c34ed8e
Hopkins, Jason
1da01369-84a8-4c55-8577-ed6c37a07037
Lefebvre, Stephane C.
ceed4c2e-0543-4ed7-9827-7919853f8b2f
Metodiev, Metodi V.
ce078e77-0492-400a-b8ff-9c04f522bf8e
Oxborough, Kevin
16d1da54-ed48-400e-9acf-d03536a1401e
Raines, Christine A.
8d94a3f5-e2c5-40be-9741-a76c7144ec91
Lawson, Tracy
6b62f544-1615-4620-b2f2-a3ce846495b7
Geider, Richard J.
f1432d5c-8c1d-48ab-ac52-e81ee5ce7f42

McKew, Boyd A., Davey, Phillip, Finch, Stewart J., Hopkins, Jason, Lefebvre, Stephane C., Metodiev, Metodi V., Oxborough, Kevin, Raines, Christine A., Lawson, Tracy and Geider, Richard J. (2013) The trade-off between the light-harvesting and photoprotective functions of fucoxanthin-chlorophyll proteins dominates light acclimation in Emiliania huxleyi (clone CCMP 1516). New Phytologist, 200 (1), 74-85. (doi:10.1111/nph.12373).

Record type: Article

Abstract

Mechanistic understanding of the costs and benefits of photoacclimation requires knowledge of how photophysiology is affected by changes in the molecular structure of the chloroplast.

We tested the hypothesis that changes in the light dependencies of photosynthesis, nonphotochemical quenching and PSII photoinactivation arises from changes in the abundances of chloroplast proteins in Emiliania huxleyi strain CCMP 1516 grown at 30 (Low Light; LL) and 1000 (High Light; HL) µmol photons m-2 s-1 photon flux densities.

Carbon-specific light-saturated gross photosynthesis rates were not significantly different between cells acclimated to LL and HL. Acclimation to LL benefited cells by increasing biomass-specific light absorption and gross photosynthesis rates under low light, whereas acclimation to HL benefited cells by reducing the rate of photoinactivation of PSII under high light. Differences in the relative abundances of proteins assigned to light-harvesting (Lhcf), photoprotection (LI818-like), and the photosystem II (PSII) core complex accompanied differences in photophysiology: specifically, Lhcf:PSII was greater under LL, whereas LI818:PSII was greater in HL.

Thus, photoacclimation in E. huxleyi involved a trade-off amongst the characteristics of light absorption and photoprotection, which could be attributed to changes in the abundance and composition of proteins in the light-harvesting antenna of PSII.

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More information

Published date: October 2013
Keywords: Emiliania huxleyi, gross photosynthesis, light acclimation, light harvesting, nonphotochemical quenching, photoinactivation, photoinhibition
Organisations: Ocean and Earth Science

Identifiers

Local EPrints ID: 358772
URI: http://eprints.soton.ac.uk/id/eprint/358772
ISSN: 0028-646X
PURE UUID: f0b88327-54a7-4821-a571-59bb93aa7479

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Date deposited: 10 Oct 2013 12:16
Last modified: 09 Jan 2022 08:31

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Contributors

Author: Boyd A. McKew
Author: Phillip Davey
Author: Stewart J. Finch
Author: Jason Hopkins
Author: Stephane C. Lefebvre
Author: Metodi V. Metodiev
Author: Kevin Oxborough
Author: Christine A. Raines
Author: Tracy Lawson
Author: Richard J. Geider

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