Analysis methods and reference concentrations of 12 minor and trace elements in fish blood plasma

Sturrock, Anna M., Hunter, Ewan, Milton, J. Andy and Trueman, Clive N. (2013) Analysis methods and reference concentrations of 12 minor and trace elements in fish blood plasma. Journal of Trace Elements in Medicine and Biology, 27 (4), 273-285. (doi:10.1016/j.jtemb.2013.03.001).

Abstract

A comprehensive review of the analytical literature revealed substantial under-representation of trace element concentrations in fish blood, particularly for marine species. We describe a simple dilution procedure to measure Li, Mg, K, Ca, Mn, Cu, Zn, Se, Rb, Sr, Ba and Pb concentrations in low volumes of blood plasma of adult plaice (Pleuronectes platessa) using high resolution-inductively coupled plasma-mass spectrometry (HR-ICP-MS). Captive male and female plaice (n = 18) were serially sampled for one year and samples collected outside of the spawning season (n = 157) used to estimate reference ranges for this species. Method accuracy was deemed satisfactory, based on its application to the analysis of a certified reference material. Precision was generally <3%, with the most conservative measure of precision being ?10% for all elements except Pb (?20%). This is the first study to analyse fish blood plasma by ICP-MS and includes some of the first reference ranges for trace element concentrations in fish blood.

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Contributors

Author: J. Andy Milton

Author: Clive N. Trueman

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