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Analysis methods and reference concentrations of 12 minor and trace elements in fish blood plasma

Analysis methods and reference concentrations of 12 minor and trace elements in fish blood plasma
Analysis methods and reference concentrations of 12 minor and trace elements in fish blood plasma
A comprehensive review of the analytical literature revealed substantial under-representation of trace element concentrations in fish blood, particularly for marine species. We describe a simple dilution procedure to measure Li, Mg, K, Ca, Mn, Cu, Zn, Se, Rb, Sr, Ba and Pb concentrations in low volumes of blood plasma of adult plaice (Pleuronectes platessa) using high resolution-inductively coupled plasma-mass spectrometry (HR-ICP-MS). Captive male and female plaice (n = 18) were serially sampled for one year and samples collected outside of the spawning season (n = 157) used to estimate reference ranges for this species. Method accuracy was deemed satisfactory, based on its application to the analysis of a certified reference material. Precision was generally <3%, with the most conservative measure of precision being ?10% for all elements except Pb (?20%). This is the first study to analyse fish blood plasma by ICP-MS and includes some of the first reference ranges for trace element concentrations in fish blood.
Fish, Multi-elemental, Blood, Plasma, Microchemistry
0946-672X
273-285
Sturrock, Anna M.
f93af2bc-7539-4f77-8247-4cfb0fbf2f94
Hunter, Ewan
75352862-16aa-44ee-aa31-ff56bf2305ea
Milton, J. Andy
9e183221-d0d4-4ddb-aeba-0fdde9d31230
Trueman, Clive N.
d00d3bd6-a47b-4d47-89ae-841c3d506205
Sturrock, Anna M.
f93af2bc-7539-4f77-8247-4cfb0fbf2f94
Hunter, Ewan
75352862-16aa-44ee-aa31-ff56bf2305ea
Milton, J. Andy
9e183221-d0d4-4ddb-aeba-0fdde9d31230
Trueman, Clive N.
d00d3bd6-a47b-4d47-89ae-841c3d506205

Sturrock, Anna M., Hunter, Ewan, Milton, J. Andy and Trueman, Clive N. (2013) Analysis methods and reference concentrations of 12 minor and trace elements in fish blood plasma. Journal of Trace Elements in Medicine and Biology, 27 (4), 273-285. (doi:10.1016/j.jtemb.2013.03.001).

Record type: Article

Abstract

A comprehensive review of the analytical literature revealed substantial under-representation of trace element concentrations in fish blood, particularly for marine species. We describe a simple dilution procedure to measure Li, Mg, K, Ca, Mn, Cu, Zn, Se, Rb, Sr, Ba and Pb concentrations in low volumes of blood plasma of adult plaice (Pleuronectes platessa) using high resolution-inductively coupled plasma-mass spectrometry (HR-ICP-MS). Captive male and female plaice (n = 18) were serially sampled for one year and samples collected outside of the spawning season (n = 157) used to estimate reference ranges for this species. Method accuracy was deemed satisfactory, based on its application to the analysis of a certified reference material. Precision was generally <3%, with the most conservative measure of precision being ?10% for all elements except Pb (?20%). This is the first study to analyse fish blood plasma by ICP-MS and includes some of the first reference ranges for trace element concentrations in fish blood.

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More information

Published date: October 2013
Keywords: Fish, Multi-elemental, Blood, Plasma, Microchemistry
Organisations: Geochemistry

Identifiers

Local EPrints ID: 358784
URI: http://eprints.soton.ac.uk/id/eprint/358784
ISSN: 0946-672X
PURE UUID: 9c6b5960-eb30-4c1f-86db-f2c1153bf86f
ORCID for J. Andy Milton: ORCID iD orcid.org/0000-0003-4245-5532
ORCID for Clive N. Trueman: ORCID iD orcid.org/0000-0002-4995-736X

Catalogue record

Date deposited: 10 Oct 2013 13:38
Last modified: 15 Mar 2024 03:17

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Contributors

Author: Anna M. Sturrock
Author: Ewan Hunter
Author: J. Andy Milton ORCID iD

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