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The effectiveness of fish oil supplementation in asthmatic rats is limited by an inefficient action on ASM function

The effectiveness of fish oil supplementation in asthmatic rats is limited by an inefficient action on ASM function
The effectiveness of fish oil supplementation in asthmatic rats is limited by an inefficient action on ASM function
Episodes of acute exacerbation are the major clinical feature of asthma and therefore represent an important focus for developing novel therapies for this disease. There are many reports that the n-3 fatty acids found in fish oil exert anti-inflammatory effects, but there are few studies of the action of fish oil on airway smooth muscle (ASM) function. In the present investigation, we evaluated the effect of fish oil supplementation on smooth muscle force of contraction in ovalbumin-induced asthmatic Wistar rats, and its consequences on static lung compliance, mucus production, leukocyte chemotaxis and production of proinflammatory cytokines. Fish oil supplementation suppressed the infiltration of inflammatory cells into the lung in asthmatic animals (2.04 ± 0.19 × 10(6) cells vs. 3.33 ± 0.43 × 10(6) cells in the control asthmatic group; P < 0.05). Static lung compliance increased with fish oil supplementation in asthmatic rats (0.640 ± 0.053 mL/cm H2O vs. 0.399 ± 0.043 mL/cm H2O; P < 0.05). However, fish oil did not prevent asthma-associated lung eosinophilia and did not affect the concentrations of tumor necrosis factor-? and interleukin-1? in lung tissue or the proportion of the airways obliterated with mucus. Fish oil had no effect on the force of contraction in asthmatic rats in response to acetylcholine (3.026 ± 0.274 mN vs. 2.813 ± 0.364 mN in the control asthmatic group). In conclusion, although fish oil exerts some benefits in this model of asthma, its effectiveness appears to be limited by an inefficient action on airway smooth muscle function.
0024-4201
889-897
Miranda, D.T.
686682d2-2d5a-44eb-b092-88475952c439
Zanatta, A.L.
753e71ea-dbba-4b1d-8416-8ebac202f5fa
Dias, B.C.
0140a82f-fc9e-4eb7-b217-a14c1d17a384
Fogaça, R.T.
8957c187-0a35-486c-befa-7a8160b6ece6
Maurer, J.B.
104c9482-71ac-4323-8490-f51ecb49e822
Donatti, L.
44869e6d-5858-4890-baf9-f7ae539db1b0
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Nishiyama, A.
a5d17b06-7790-44f3-80b4-1e33cc4446e9
Miranda, D.T.
686682d2-2d5a-44eb-b092-88475952c439
Zanatta, A.L.
753e71ea-dbba-4b1d-8416-8ebac202f5fa
Dias, B.C.
0140a82f-fc9e-4eb7-b217-a14c1d17a384
Fogaça, R.T.
8957c187-0a35-486c-befa-7a8160b6ece6
Maurer, J.B.
104c9482-71ac-4323-8490-f51ecb49e822
Donatti, L.
44869e6d-5858-4890-baf9-f7ae539db1b0
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Nishiyama, A.
a5d17b06-7790-44f3-80b4-1e33cc4446e9

Miranda, D.T., Zanatta, A.L., Dias, B.C., Fogaça, R.T., Maurer, J.B., Donatti, L., Calder, Philip C. and Nishiyama, A. (2013) The effectiveness of fish oil supplementation in asthmatic rats is limited by an inefficient action on ASM function. Lipids, 48 (9), 889-897. (doi:10.1007/s11745-013-3804-4). (PMID:23743575)

Record type: Article

Abstract

Episodes of acute exacerbation are the major clinical feature of asthma and therefore represent an important focus for developing novel therapies for this disease. There are many reports that the n-3 fatty acids found in fish oil exert anti-inflammatory effects, but there are few studies of the action of fish oil on airway smooth muscle (ASM) function. In the present investigation, we evaluated the effect of fish oil supplementation on smooth muscle force of contraction in ovalbumin-induced asthmatic Wistar rats, and its consequences on static lung compliance, mucus production, leukocyte chemotaxis and production of proinflammatory cytokines. Fish oil supplementation suppressed the infiltration of inflammatory cells into the lung in asthmatic animals (2.04 ± 0.19 × 10(6) cells vs. 3.33 ± 0.43 × 10(6) cells in the control asthmatic group; P < 0.05). Static lung compliance increased with fish oil supplementation in asthmatic rats (0.640 ± 0.053 mL/cm H2O vs. 0.399 ± 0.043 mL/cm H2O; P < 0.05). However, fish oil did not prevent asthma-associated lung eosinophilia and did not affect the concentrations of tumor necrosis factor-? and interleukin-1? in lung tissue or the proportion of the airways obliterated with mucus. Fish oil had no effect on the force of contraction in asthmatic rats in response to acetylcholine (3.026 ± 0.274 mN vs. 2.813 ± 0.364 mN in the control asthmatic group). In conclusion, although fish oil exerts some benefits in this model of asthma, its effectiveness appears to be limited by an inefficient action on airway smooth muscle function.

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Published date: 7 June 2013
Organisations: Human Development & Health

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Local EPrints ID: 359010
URI: http://eprints.soton.ac.uk/id/eprint/359010
ISSN: 0024-4201
PURE UUID: dc74f5ef-1f27-43b7-a69b-4029303f902a
ORCID for Philip C. Calder: ORCID iD orcid.org/0000-0002-6038-710X

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Date deposited: 18 Oct 2013 12:52
Last modified: 23 Jul 2022 01:39

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Contributors

Author: D.T. Miranda
Author: A.L. Zanatta
Author: B.C. Dias
Author: R.T. Fogaça
Author: J.B. Maurer
Author: L. Donatti
Author: A. Nishiyama

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