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Single-channel electrophysiology of cell-free expressed ion channels by direct incorporation in lipid bilayers

Single-channel electrophysiology of cell-free expressed ion channels by direct incorporation in lipid bilayers
Single-channel electrophysiology of cell-free expressed ion channels by direct incorporation in lipid bilayers
Single-channel electrophysiology with lipid bilayer systems requires ion channel expression, purification from cell culture, and reconstitution in proteoliposomes for delivery to a planar bilayer. Here we demonstrate that single-channel current measurements of the potassium channels KcsA and hERGS5-S6 can be obtained by direct insertion in interdroplet lipid bilayers from microliters of a cell-free expression medium.
0003-2654
7294-7298
Friddin, Mark S.
d3e84f8a-c24b-49b9-908c-c3fe612b39b0
Smithers, Natalie P.
63ead01b-6515-4f82-a963-884f572af872
Beaugrand, Maïwenn
01de7840-d85e-4d5a-a556-45258a37acfc
Marcotte, Isabelle
6908440c-f797-48ee-b109-cfa557552ff2
Williamson, Philip T.F.
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Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
de Planque, Maurits R.R.
a1d33d13-f516-44fb-8d2c-c51d18bc21ba
Friddin, Mark S.
d3e84f8a-c24b-49b9-908c-c3fe612b39b0
Smithers, Natalie P.
63ead01b-6515-4f82-a963-884f572af872
Beaugrand, Maïwenn
01de7840-d85e-4d5a-a556-45258a37acfc
Marcotte, Isabelle
6908440c-f797-48ee-b109-cfa557552ff2
Williamson, Philip T.F.
0b7715c6-b60e-4e95-a1b1-6afc8b9f372a
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
de Planque, Maurits R.R.
a1d33d13-f516-44fb-8d2c-c51d18bc21ba

Friddin, Mark S., Smithers, Natalie P., Beaugrand, Maïwenn, Marcotte, Isabelle, Williamson, Philip T.F., Morgan, Hywel and de Planque, Maurits R.R. (2013) Single-channel electrophysiology of cell-free expressed ion channels by direct incorporation in lipid bilayers. Analyst, 138 (24), 7294-7298. (doi:10.1039/C3AN01540H).

Record type: Article

Abstract

Single-channel electrophysiology with lipid bilayer systems requires ion channel expression, purification from cell culture, and reconstitution in proteoliposomes for delivery to a planar bilayer. Here we demonstrate that single-channel current measurements of the potassium channels KcsA and hERGS5-S6 can be obtained by direct insertion in interdroplet lipid bilayers from microliters of a cell-free expression medium.

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e-pub ahead of print date: 18 October 2013
Organisations: Molecular and Cellular, Nanoelectronics and Nanotechnology

Identifiers

Local EPrints ID: 359046
URI: https://eprints.soton.ac.uk/id/eprint/359046
ISSN: 0003-2654
PURE UUID: 517ea679-7bcd-4c96-8286-d7ee22703e1b
ORCID for Philip T.F. Williamson: ORCID iD orcid.org/0000-0002-0231-8640
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676
ORCID for Maurits R.R. de Planque: ORCID iD orcid.org/0000-0002-8787-0513

Catalogue record

Date deposited: 21 Oct 2013 09:16
Last modified: 20 Jul 2019 00:59

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Contributors

Author: Mark S. Friddin
Author: Natalie P. Smithers
Author: Maïwenn Beaugrand
Author: Isabelle Marcotte
Author: Hywel Morgan ORCID iD

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