Nerve pathology in the type 1 diabetic dog: effects of treatment with sulindac
Nerve pathology in the type 1 diabetic dog: effects of treatment with sulindac
The purpose of this study was to define pathological abnormalities in the peripheral nerve of a large animal model of long-duration type 1 diabetes and also to determine the effects of treatment with sulindac. Detailed morphometric studies were performed to define nerve fiber and endoneurial capillary pathology in 6 control dogs, 6 type 1 diabetic dogs treated with insulin, and 6 type 1 diabetic dogs treated with insulin and sulindac for 4 years. Myelinated fiber and regenerative cluster density showed a non-significant trend toward a reduction in diabetic compared to control animals, which was prevented by treatment with sulindac. Unmyelinated fiber density did not differ among groups. However, diabetic animals showed a non-significant trend toward an increase in axon diameter (p < 0.07), with a shift of the size frequency distribution towards larger axons, which was not prevented by treatment with sulindac. Endoneurial capillary density and luminal area showed a non-significant trend toward an increase in diabetic animals, which was prevented with sulindac treatment. Endoneurial capillary basement membrane area was significantly increased (p < 0.05) in diabetic animals, but was not prevented with sulindac treatment. We conclude that the type 1 diabetic dog demonstrates minor structural abnormalities in the nerve fibers and endoneurial capillaries of the sciatic nerve, and treatment with sulindac ameliorates some but not all of these abnormalities.
type 1 diabetes, neuropathy, morphometry, nerve fiber, endoneurial capillary, sulindac
219-226
Walker, David
b94e6f46-ec02-4b5b-a726-67c0aafc9f94
Siddique, Irfan
d694aff6-0d02-4a76-b5e7-61654598007e
Anderson, Heather
5c16d9c2-2ecb-441a-b498-e18e523e76b6
Gardiner, Tom A.
fa79104d-e605-4de0-b079-84d109ca7a97
Archer, Desmond B.
4c74b832-b6a5-4a87-b6f8-a80b92e27c79
Boulton, Andrew J.M.
67c214d5-db6d-4d05-b8ae-31348a9f4637
Malik, Rayaz A.
71dac276-5d84-439d-a380-faa0285feb86
December 2001
Walker, David
b94e6f46-ec02-4b5b-a726-67c0aafc9f94
Siddique, Irfan
d694aff6-0d02-4a76-b5e7-61654598007e
Anderson, Heather
5c16d9c2-2ecb-441a-b498-e18e523e76b6
Gardiner, Tom A.
fa79104d-e605-4de0-b079-84d109ca7a97
Archer, Desmond B.
4c74b832-b6a5-4a87-b6f8-a80b92e27c79
Boulton, Andrew J.M.
67c214d5-db6d-4d05-b8ae-31348a9f4637
Malik, Rayaz A.
71dac276-5d84-439d-a380-faa0285feb86
Walker, David, Siddique, Irfan, Anderson, Heather, Gardiner, Tom A., Archer, Desmond B., Boulton, Andrew J.M. and Malik, Rayaz A.
(2001)
Nerve pathology in the type 1 diabetic dog: effects of treatment with sulindac.
Journal of the Peripheral Nervous System, 6 (4), .
(doi:10.1046/j.1529-8027.2001.01023.x).
(PMID:11800045)
Abstract
The purpose of this study was to define pathological abnormalities in the peripheral nerve of a large animal model of long-duration type 1 diabetes and also to determine the effects of treatment with sulindac. Detailed morphometric studies were performed to define nerve fiber and endoneurial capillary pathology in 6 control dogs, 6 type 1 diabetic dogs treated with insulin, and 6 type 1 diabetic dogs treated with insulin and sulindac for 4 years. Myelinated fiber and regenerative cluster density showed a non-significant trend toward a reduction in diabetic compared to control animals, which was prevented by treatment with sulindac. Unmyelinated fiber density did not differ among groups. However, diabetic animals showed a non-significant trend toward an increase in axon diameter (p < 0.07), with a shift of the size frequency distribution towards larger axons, which was not prevented by treatment with sulindac. Endoneurial capillary density and luminal area showed a non-significant trend toward an increase in diabetic animals, which was prevented with sulindac treatment. Endoneurial capillary basement membrane area was significantly increased (p < 0.05) in diabetic animals, but was not prevented with sulindac treatment. We conclude that the type 1 diabetic dog demonstrates minor structural abnormalities in the nerve fibers and endoneurial capillaries of the sciatic nerve, and treatment with sulindac ameliorates some but not all of these abnormalities.
This record has no associated files available for download.
More information
Published date: December 2001
Keywords:
type 1 diabetes, neuropathy, morphometry, nerve fiber, endoneurial capillary, sulindac
Organisations:
Medical Education
Identifiers
Local EPrints ID: 359169
URI: http://eprints.soton.ac.uk/id/eprint/359169
ISSN: 1085-9489
PURE UUID: 776a18b3-b7ab-4fc1-8f81-1cb61d5e3ccd
Catalogue record
Date deposited: 23 Oct 2013 10:18
Last modified: 14 Mar 2024 15:17
Export record
Altmetrics
Contributors
Author:
Irfan Siddique
Author:
Heather Anderson
Author:
Tom A. Gardiner
Author:
Desmond B. Archer
Author:
Andrew J.M. Boulton
Author:
Rayaz A. Malik
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics