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A high performance microfluidic analyser for phosphate measurements in marine waters using the vanadomolybdate method

A high performance microfluidic analyser for phosphate measurements in marine waters using the vanadomolybdate method
A high performance microfluidic analyser for phosphate measurements in marine waters using the vanadomolybdate method
We report a high performance autonomous analytical system based on the vanadomolybdate method for the determination of soluble reactive phosphorus in seawater. The system combines a microfluidic chip manufactured from tinted poly (methyl methacrylate) (PMMA), a custom made syringe pump, embedded control electronics and on-board calibration standards. This “lab-on-a-chip” analytical system was successfully deployed and cross-compared with reference analytical methods in coastal (south west England) and open ocean waters (tropical North Atlantic). The results of the miniaturized system compared well with a reference bench-operated phosphate auto-analyser and showed no significant differences in the analytical results (student’s t-test at 95% confidence level). The optical technology used, comprising of tinted PMMA and polished fluidic channels, has allowed an improvement of two orders of magnitude of the limit of detection (52 nM) compared to currently available portable systems based on this method. The system has a wide linear dynamic range 0.1–60 µM, and a good precision (13.6% at 0.4 µM, n=4). The analytical results were corrected for silicate interferences at 0.7 µM, and the measurement frequency was configurable with a sampling throughput of up to 20 samples per hour. This portable micro-analytical system has a low reagent requirement (340 µL per sample) and power consumption (756 J per sample), and has allowed accurate high resolution measurements of soluble reactive phosphorus in seawater.
Phosphate measurement, Nutrient analysis, Microfluidic analyser, Vanadomolybdate method, Silicate interference, Seawater
0039-9140
382-387
Legiret, François-Eric
954da4a6-fb75-4899-b8c9-04bed2749f89
Sieben, Vincent J.
7c2fe2a3-4b9c-4359-835e-ea375b25d30f
Woodward, E. Malcolm S.
3a11b7bf-b110-448a-b1ee-ae1ce7d0fef5
Abi Kaed Bey, Samer K.
a3568a3d-a024-4dd8-9ac3-87f324bf0821
Mowlem, Matthew C.
6f633ca2-298f-48ee-a025-ce52dd62124f
Connelly, Douglas P.
d49131bb-af38-4768-9953-7ae0b43e33c8
Achterberg, Eric P.
685ce961-8c45-4503-9f03-50f6561202b9
Legiret, François-Eric
954da4a6-fb75-4899-b8c9-04bed2749f89
Sieben, Vincent J.
7c2fe2a3-4b9c-4359-835e-ea375b25d30f
Woodward, E. Malcolm S.
3a11b7bf-b110-448a-b1ee-ae1ce7d0fef5
Abi Kaed Bey, Samer K.
a3568a3d-a024-4dd8-9ac3-87f324bf0821
Mowlem, Matthew C.
6f633ca2-298f-48ee-a025-ce52dd62124f
Connelly, Douglas P.
d49131bb-af38-4768-9953-7ae0b43e33c8
Achterberg, Eric P.
685ce961-8c45-4503-9f03-50f6561202b9

Legiret, François-Eric, Sieben, Vincent J., Woodward, E. Malcolm S., Abi Kaed Bey, Samer K., Mowlem, Matthew C., Connelly, Douglas P. and Achterberg, Eric P. (2013) A high performance microfluidic analyser for phosphate measurements in marine waters using the vanadomolybdate method. Talanta, 116, 382-387. (doi:10.1016/j.talanta.2013.05.004).

Record type: Article

Abstract

We report a high performance autonomous analytical system based on the vanadomolybdate method for the determination of soluble reactive phosphorus in seawater. The system combines a microfluidic chip manufactured from tinted poly (methyl methacrylate) (PMMA), a custom made syringe pump, embedded control electronics and on-board calibration standards. This “lab-on-a-chip” analytical system was successfully deployed and cross-compared with reference analytical methods in coastal (south west England) and open ocean waters (tropical North Atlantic). The results of the miniaturized system compared well with a reference bench-operated phosphate auto-analyser and showed no significant differences in the analytical results (student’s t-test at 95% confidence level). The optical technology used, comprising of tinted PMMA and polished fluidic channels, has allowed an improvement of two orders of magnitude of the limit of detection (52 nM) compared to currently available portable systems based on this method. The system has a wide linear dynamic range 0.1–60 µM, and a good precision (13.6% at 0.4 µM, n=4). The analytical results were corrected for silicate interferences at 0.7 µM, and the measurement frequency was configurable with a sampling throughput of up to 20 samples per hour. This portable micro-analytical system has a low reagent requirement (340 µL per sample) and power consumption (756 J per sample), and has allowed accurate high resolution measurements of soluble reactive phosphorus in seawater.

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More information

Published date: 15 November 2013
Keywords: Phosphate measurement, Nutrient analysis, Microfluidic analyser, Vanadomolybdate method, Silicate interference, Seawater
Organisations: Ocean and Earth Science, Electronics & Computer Science, Marine Geoscience, Ocean Technology and Engineering

Identifiers

Local EPrints ID: 359569
URI: http://eprints.soton.ac.uk/id/eprint/359569
ISSN: 0039-9140
PURE UUID: 6de24e8c-7a8a-4aaa-9ee4-9ef76336fbe9

Catalogue record

Date deposited: 04 Nov 2013 14:53
Last modified: 16 Jul 2019 21:18

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