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Colloidal quantum dots: the opportunities and the pitfalls for DNA analysis applications

Colloidal quantum dots: the opportunities and the pitfalls for DNA analysis applications
Colloidal quantum dots: the opportunities and the pitfalls for DNA analysis applications
Colloidal quantum dots (QDs) have received considerable attention as luminescent probes for DNA analysis applications. The underlying photophysical and photochemical properties of these probes need consideration when designing assays for DNA analysis. These properties include intermittent fluorescence often termed 'blinking', photobleaching, photoinduced fluorescence enhancement and, as a result of recent evidence, photo-induced generation of reactive oxygen species leading to DNA damage. Even though the design of assays for DNA analysis using QDs needs care, QDs do provide advantages over fluorophores for many emerging DNA analysis methods where low copy numbers of DNA are present. Many of the more traditional DNA assay methods using fluorophore labeled probes either cannot be translated, or show no benefit in using QDs as the lumophore.
978-0-8412-2828-3
113
323-364
Oxford University Press
Pechstedt, K.
5e108ee2-e41b-4d05-8e18-ead38d018d5f
Melvin, T.
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Hepel, M.R.
Zhang, C-J
Pechstedt, K.
5e108ee2-e41b-4d05-8e18-ead38d018d5f
Melvin, T.
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Hepel, M.R.
Zhang, C-J

Pechstedt, K. and Melvin, T. (2013) Colloidal quantum dots: the opportunities and the pitfalls for DNA analysis applications. In, Hepel, M.R. and Zhang, C-J (eds.) Functional Nanoparticles for Bioanalysis, Nanomedicine, and Bioelectronic Devices Volume 2. (ACS Symposium Series 1, , (doi:10.1021/bk-2012-1113.ch013), 113) Oxford University Press, pp. 323-364. (doi:10.1021/bk-2012-1113.ch013).

Record type: Book Section

Abstract

Colloidal quantum dots (QDs) have received considerable attention as luminescent probes for DNA analysis applications. The underlying photophysical and photochemical properties of these probes need consideration when designing assays for DNA analysis. These properties include intermittent fluorescence often termed 'blinking', photobleaching, photoinduced fluorescence enhancement and, as a result of recent evidence, photo-induced generation of reactive oxygen species leading to DNA damage. Even though the design of assays for DNA analysis using QDs needs care, QDs do provide advantages over fluorophores for many emerging DNA analysis methods where low copy numbers of DNA are present. Many of the more traditional DNA assay methods using fluorophore labeled probes either cannot be translated, or show no benefit in using QDs as the lumophore.

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Published date: 14 March 2013
Organisations: Optoelectronics Research Centre

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Local EPrints ID: 359629
URI: http://eprints.soton.ac.uk/id/eprint/359629
ISBN: 978-0-8412-2828-3
PURE UUID: c03d0ef0-2971-458b-bcbb-f35b1472ed8a

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Date deposited: 08 Nov 2013 08:55
Last modified: 22 Jul 2019 16:31

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Contributors

Author: K. Pechstedt
Author: T. Melvin
Editor: M.R. Hepel
Editor: C-J Zhang

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