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Placental malaria is associated with attenuated CD4 T-cell responses to tuberculin PPD 12 months after BCG vaccination

Placental malaria is associated with attenuated CD4 T-cell responses to tuberculin PPD 12 months after BCG vaccination
Placental malaria is associated with attenuated CD4 T-cell responses to tuberculin PPD 12 months after BCG vaccination
Background
Placental malaria (PM) is associated with prenatal malaise, but many PM+ infants are born without symptoms. As malaria has powerful immunomodulatory effects, we tested the hypothesis that PM predicts reduced T-cell responses to vaccine challenge.

Methods
We recruited healthy PM+ and PM- infants at birth. At six and 12 months, we stimulated PBMCs with tuberculin purified protein derivative (PPD) and compared expression of CD154, IL-2 and IFN? by CD4 T-cells to a negative control using flow cytometry.

We measured the length, weight and head circumference at birth and 12 months.

Results
IL-2 and CD154 expression were low in both groups at both timepoints, without discernable differences. Expression of IFN? was similarly low at 6 months but by 12 months, the median response was higher in PM- than PM + infants (p = 0.026). The PM+ infants also had a lower weight (p = 0.032) and head circumference (p = 0.041) at 12 months, indicating lower growth rates.

At birth, the size and weight of the PM+ and PM- infants were equivalent. By 12 months, the PM+ infants had a lower weight and head circumference than the PM- infants.

Conclusions
Placental malaria was associated with reduced immune responses 12 months after immune challenge in infants apparently healthy at birth.

1471-2334
6
Walther, B.
58f903c5-3f39-4ccc-902c-87c94f6fb637
Miles, D.J.
7a826bc7-614a-4b13-b288-65e0df3eb772
Waight, P.
9c244d2d-c6a3-4d6f-9da4-7be6e8132a8f
Palmero, M.S.
416a1543-71c3-4bdf-b370-98f49c34837b
Ojuola, O.
e39780a0-1961-454d-9bd9-2f4155fac1ad
Touray, E.S.
8aedbf82-aea5-45ca-bdd7-f18624e64df9
Whittle, H.
aae12997-cc35-4ec7-933b-5afceedd926c
van der Sande, M.
d144b420-e5b5-4ff9-889b-189a39090b7c
Crozier, S.
1f39648f-d527-497f-bb40-319f487a0782
Flanagan, K.L.
0403b22b-a406-43ac-82c1-582002dcc656
Walther, B.
58f903c5-3f39-4ccc-902c-87c94f6fb637
Miles, D.J.
7a826bc7-614a-4b13-b288-65e0df3eb772
Waight, P.
9c244d2d-c6a3-4d6f-9da4-7be6e8132a8f
Palmero, M.S.
416a1543-71c3-4bdf-b370-98f49c34837b
Ojuola, O.
e39780a0-1961-454d-9bd9-2f4155fac1ad
Touray, E.S.
8aedbf82-aea5-45ca-bdd7-f18624e64df9
Whittle, H.
aae12997-cc35-4ec7-933b-5afceedd926c
van der Sande, M.
d144b420-e5b5-4ff9-889b-189a39090b7c
Crozier, S.
1f39648f-d527-497f-bb40-319f487a0782
Flanagan, K.L.
0403b22b-a406-43ac-82c1-582002dcc656

Walther, B., Miles, D.J., Waight, P., Palmero, M.S., Ojuola, O., Touray, E.S., Whittle, H., van der Sande, M., Crozier, S. and Flanagan, K.L. (2012) Placental malaria is associated with attenuated CD4 T-cell responses to tuberculin PPD 12 months after BCG vaccination. BMC Infectious Diseases, 12, 6. (doi:10.1186/1471-2334-12-6). (PMID:22243970)

Record type: Article

Abstract

Background
Placental malaria (PM) is associated with prenatal malaise, but many PM+ infants are born without symptoms. As malaria has powerful immunomodulatory effects, we tested the hypothesis that PM predicts reduced T-cell responses to vaccine challenge.

Methods
We recruited healthy PM+ and PM- infants at birth. At six and 12 months, we stimulated PBMCs with tuberculin purified protein derivative (PPD) and compared expression of CD154, IL-2 and IFN? by CD4 T-cells to a negative control using flow cytometry.

We measured the length, weight and head circumference at birth and 12 months.

Results
IL-2 and CD154 expression were low in both groups at both timepoints, without discernable differences. Expression of IFN? was similarly low at 6 months but by 12 months, the median response was higher in PM- than PM + infants (p = 0.026). The PM+ infants also had a lower weight (p = 0.032) and head circumference (p = 0.041) at 12 months, indicating lower growth rates.

At birth, the size and weight of the PM+ and PM- infants were equivalent. By 12 months, the PM+ infants had a lower weight and head circumference than the PM- infants.

Conclusions
Placental malaria was associated with reduced immune responses 12 months after immune challenge in infants apparently healthy at birth.

Full text not available from this repository.

More information

Published date: 14 January 2012
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 359656
URI: http://eprints.soton.ac.uk/id/eprint/359656
ISSN: 1471-2334
PURE UUID: 8c5ea5ca-5a40-4f00-89fe-655bfd6562f7

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Date deposited: 12 Nov 2013 10:15
Last modified: 16 Jul 2019 21:17

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Contributors

Author: B. Walther
Author: D.J. Miles
Author: P. Waight
Author: M.S. Palmero
Author: O. Ojuola
Author: E.S. Touray
Author: H. Whittle
Author: M. van der Sande
Author: S. Crozier
Author: K.L. Flanagan

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